中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2010年
6期
359-363
,共5页
杜文军%韩绍磊%徐燕%秦来英%陈士俊
杜文軍%韓紹磊%徐燕%秦來英%陳士俊
두문군%한소뢰%서연%진래영%진사준
白细胞介素17%肝炎,乙型,慢性%肝硬化
白細胞介素17%肝炎,乙型,慢性%肝硬化
백세포개소17%간염,을형,만성%간경화
Interleukin-17%Hepatitis B,chronic%Liver cirrhosis
目的 探讨IL-17肝内表达与慢性HBV感染所致肝纤维化的相关性.方法 免疫组织化学法测定30例慢性HBV携带者、55例慢性乙型肝炎患者、20例乙型肝炎肝硬化患者肝组织内不同炎症程度分级和肝纤维化分期中IL-17的表达,ELISA法测定血清IL-17及肝纤维化指标HA、LN、PCⅢ、ⅣC的水平.组间差异性检验采用Kruskal-Wallis检验和Mann-Whitney检验,相关性分析采用Spearman分析.结果 肝组织IL-17表达水平在肝硬化组高于慢性乙型肝炎组(x2=25.3982,P=0.004),在慢性乙型肝炎组高于慢性HBV携带组(x2=11.5056,P=0.001);不同炎症程度及纤维化分级与IL-17表达强度呈正相关(r=0.718、0.693,均P＜0.01);肝组织IL-17主要集中于汇管区,其表达强度与血清HA、LN、PCⅢ、ⅣC呈正相关(r=0.793、0.834、0.722、0.883,均P＜0.01).结论 IL-17的肝内表达与肝内炎症程度分级及肝纤维化程度密切相关.
目的 探討IL-17肝內錶達與慢性HBV感染所緻肝纖維化的相關性.方法 免疫組織化學法測定30例慢性HBV攜帶者、55例慢性乙型肝炎患者、20例乙型肝炎肝硬化患者肝組織內不同炎癥程度分級和肝纖維化分期中IL-17的錶達,ELISA法測定血清IL-17及肝纖維化指標HA、LN、PCⅢ、ⅣC的水平.組間差異性檢驗採用Kruskal-Wallis檢驗和Mann-Whitney檢驗,相關性分析採用Spearman分析.結果 肝組織IL-17錶達水平在肝硬化組高于慢性乙型肝炎組(x2=25.3982,P=0.004),在慢性乙型肝炎組高于慢性HBV攜帶組(x2=11.5056,P=0.001);不同炎癥程度及纖維化分級與IL-17錶達彊度呈正相關(r=0.718、0.693,均P＜0.01);肝組織IL-17主要集中于彙管區,其錶達彊度與血清HA、LN、PCⅢ、ⅣC呈正相關(r=0.793、0.834、0.722、0.883,均P＜0.01).結論 IL-17的肝內錶達與肝內炎癥程度分級及肝纖維化程度密切相關.
목적 탐토IL-17간내표체여만성HBV감염소치간섬유화적상관성.방법 면역조직화학법측정30례만성HBV휴대자、55례만성을형간염환자、20례을형간염간경화환자간조직내불동염증정도분급화간섬유화분기중IL-17적표체,ELISA법측정혈청IL-17급간섬유화지표HA、LN、PCⅢ、ⅣC적수평.조간차이성검험채용Kruskal-Wallis검험화Mann-Whitney검험,상관성분석채용Spearman분석.결과 간조직IL-17표체수평재간경화조고우만성을형간염조(x2=25.3982,P=0.004),재만성을형간염조고우만성HBV휴대조(x2=11.5056,P=0.001);불동염증정도급섬유화분급여IL-17표체강도정정상관(r=0.718、0.693,균P＜0.01);간조직IL-17주요집중우회관구,기표체강도여혈청HA、LN、PCⅢ、ⅣC정정상관(r=0.793、0.834、0.722、0.883,균P＜0.01).결론 IL-17적간내표체여간내염증정도분급급간섬유화정도밀절상관.
Objective To explore the relationship between intrahepatic expression of interleukin-17 (IL-17) and liver fibrosis in patients with chronic hepatitis B virus infection. Methods IL-17 expressions in livers with different inflammation activity grades and hepatic fibrosis stages from patients with chronic hepatitis B virus carriers (n= 30), chronic hepatitis B (CHB, n = 55), liver cirrhosis (LC, n=20) were measured by immunohistochemistry. Serum IL-17 and liver fibrosis indices of haluronic acid (HA), laminin (LN), type Ⅲ procollagen (PC Ⅲ ) and type Ⅳ collagen ( Ⅳ C) were determined by enzyme linked immunosorbent assay (ELISA). The differences between groups were compared by Kruskal-Wallis test, and the Mann-Whitney test, and the correlation analysis was done by Spearman test. Results Intrahepatic IL-17 expression in LC group was significantly higher than CHB group (x2 =25. 3982, P=0. 004), and that in CHB group was higher than chronic hepatitis B virus carriers group (x2 = 11. 5056, P= 0. 001). The inflammation activity grade and hepatic fibrosis stage were both positively correlated with IL-17 expression (r= 0.718, 0. 693, respectively; both P＜0.01). IL-17 mainly located in portal area and the expression was positively correlated with serum levels of HA, LN, PCⅢ and ⅣC (r=0. 793, 0. 834, 0. 722, 0. 883, respectively; all P＜0.01).Conclusion Intrahepatic IL-17 expression is closely correlated with liver inflammation activity grade and hepatic fibrosis stage.
