CAJ | 학술논문

目的探讨成年人急性髓系白血病(AML)三色流式细胞术免疫分型的特点.方法采用CD45/SSC双参数散点图设门方法对126例成年AML患者进行三色流式细胞术免疫分型分析.结果髓系抗原CD13、CD33和CD117在AML各亚型中均有很高的表达,阳性率分别为86.4%、70.2%和90.4%;CD34及HLA-DR表达偏低,分别为63.5%和61.7%.约34.2%AML患者伴有淋系抗原表达,AML淋系抗原CD7和CD19表达率分别为23.6%和2.3%.结论流式细胞术免疫分型对AML的诊断及预后判断具有重要的意义.
목적 탐토성년인급성수계백혈병(AML)삼색류식세포술면역분형적특점.방법 채용CD45/SSC쌍삼수산점도설문방법 대126례성년AML환자진행삼색류식세포술면역분형분석.결과 수계항원CD13、CD33화CD117재AML각아형중균유흔고적표체,양성솔분별위86.4%、70.2%화90.4%;CD34급HLA-DR표체편저,분별위63.5%화61.7%.약34.2%AML환자반유림계항원표체,AML림계항원CD7화CD19표체솔분별위23.6%화2.3%.결론 류식세포술면역분형대AML적진단급예후판단구유중요적의의.
Objective To evaluate the characteristics of immunophenotyping in adults with acute myeloid leukemia (AML) using muhi-eolor flow cytometry. Methods Immunophenotyping was performed by three color flow cytometry using CD45/SSC gating. Results In 126 patients with AML, the myeloid antigen of CD13, CD33 and CD117 was highly expressed. The positive rate was 86.4 %, 70.2 % and 90.4 %, respectively. The CD34 and HLA-DR were lowerly expressed as 63.5 % and 61.7 %, respectively. About 34.2 % of lymphoid-assoeiated antigen expression of all the AML patients. The lymphoid-associated antigens of CD7 and CD19 expression in patients with AML was 23.6 % and 2.3 %, respectively. Conclusion Multi-color flow cytometry is an important method for diagnosis and prognosis for AML.