中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2008年
12期
1215-1217
,共3页
陈定贵%张一%张锁才%罗德宝
陳定貴%張一%張鎖纔%囉德寶
진정귀%장일%장쇄재%라덕보
乙型肝炎%白细胞介素-15%酶联免疫吸附试验
乙型肝炎%白細胞介素-15%酶聯免疫吸附試驗
을형간염%백세포개소-15%매련면역흡부시험
Type B hepatitis%Interleukin-15%ELISA
目的 观察重型乙型肝炎患者血清白细胞介素-15(IL-15)水平的变化,探讨其在重型肝炎发病机制中的可能作用及临床意义.方法 采用双抗体夹心酶联免疫吸附试验检测47例重型乙型肝炎和20例健康者血清IL-15水平,同期检测血清丙氨酸氨基转移酶(ALT)、总胆红素(TBIL)、凝血酶原活动度(PTA)等,分析重型乙型肝炎患者血清IL-15水平与ALT、TBIL、PTA等的相关性.结果 重型乙型肝炎组血清IL-15为(18.9±7.5)ng/L,显著高于正常对照组(5.9±2.0)ng/L(P<0.01);死亡组血清IL-15为(24.1±7.5)ng/L,显著高于存活组(15.7±5.4)ng/L(P<0.01);存活组患者血清IL-15浓度随一般情况好转、肝功能恢复而下降.此外,重型乙型肝炎组IL-15与TBIL呈正相关(r=0.570,P<0.01),与PTA呈负相关(r=-0.529,P<0.01),与ALT则无显著相关性(r=0.099,P>0.05).结论 IL-15可能参与重型乙型肝炎的发病过程.IL-15水平与病情存在一定程度的一致性,与病情的转归密切相关.血清IL-15水平的检测可能对于重型乙型肝炎患者的预后具有预测作用.
目的 觀察重型乙型肝炎患者血清白細胞介素-15(IL-15)水平的變化,探討其在重型肝炎髮病機製中的可能作用及臨床意義.方法 採用雙抗體夾心酶聯免疫吸附試驗檢測47例重型乙型肝炎和20例健康者血清IL-15水平,同期檢測血清丙氨痠氨基轉移酶(ALT)、總膽紅素(TBIL)、凝血酶原活動度(PTA)等,分析重型乙型肝炎患者血清IL-15水平與ALT、TBIL、PTA等的相關性.結果 重型乙型肝炎組血清IL-15為(18.9±7.5)ng/L,顯著高于正常對照組(5.9±2.0)ng/L(P<0.01);死亡組血清IL-15為(24.1±7.5)ng/L,顯著高于存活組(15.7±5.4)ng/L(P<0.01);存活組患者血清IL-15濃度隨一般情況好轉、肝功能恢複而下降.此外,重型乙型肝炎組IL-15與TBIL呈正相關(r=0.570,P<0.01),與PTA呈負相關(r=-0.529,P<0.01),與ALT則無顯著相關性(r=0.099,P>0.05).結論 IL-15可能參與重型乙型肝炎的髮病過程.IL-15水平與病情存在一定程度的一緻性,與病情的轉歸密切相關.血清IL-15水平的檢測可能對于重型乙型肝炎患者的預後具有預測作用.
목적 관찰중형을형간염환자혈청백세포개소-15(IL-15)수평적변화,탐토기재중형간염발병궤제중적가능작용급림상의의.방법 채용쌍항체협심매련면역흡부시험검측47례중형을형간염화20례건강자혈청IL-15수평,동기검측혈청병안산안기전이매(ALT)、총담홍소(TBIL)、응혈매원활동도(PTA)등,분석중형을형간염환자혈청IL-15수평여ALT、TBIL、PTA등적상관성.결과 중형을형간염조혈청IL-15위(18.9±7.5)ng/L,현저고우정상대조조(5.9±2.0)ng/L(P<0.01);사망조혈청IL-15위(24.1±7.5)ng/L,현저고우존활조(15.7±5.4)ng/L(P<0.01);존활조환자혈청IL-15농도수일반정황호전、간공능회복이하강.차외,중형을형간염조IL-15여TBIL정정상관(r=0.570,P<0.01),여PTA정부상관(r=-0.529,P<0.01),여ALT칙무현저상관성(r=0.099,P>0.05).결론 IL-15가능삼여중형을형간염적발병과정.IL-15수평여병정존재일정정도적일치성,여병정적전귀밀절상관.혈청IL-15수평적검측가능대우중형을형간염환자적예후구유예측작용.
Objective To observe the level changes of interleukin-15 (IL-15) and its role and clinical sig-nificance in severe type B hepatitis(HB). Methods IL-15 levels of 47 cases of severe HB and 20 cases of healthy subjects were detected by ELISA,meanwhile the alanine aminotransferase (ALT),total bilirubin (TBIL) and pro-thrombase activity (PTA) were measured as well. The correlation between IL-15 and ALT,TBIL and PTA were ana-lyzed. Results IL-15 in severe HB eases were higher than in control group [(18.9±7.5 ) ng/L vs. (5.9±2.0) ng/L,P <0.01] ,which was higher in death group than in survival group[(24.1±7.5) ng/L vs. (15.7±5.4) ng/L, P<0.01]. IL-15 level was decreasing with the improvement of general condition and liver function recovery. In addition, IL-15 in severe HB was positively correlated with TBIL (r=0.570,P<0.01) and negatively correlated with PTA(r=-0.529,P<0.01) but was not significantly correlated with ALT(r=0.099,P>0.05). Conclusion IL-15 may take part in the pathogenesis of severe HB ,which is consistent with disease condition and is closely re-lated to the improvement of disease. The detection of IL-15 may exert a predicting role in the prognosis of severe HB.
