中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2012年
20期
1428-1430
,共3页
赵京玉%柴家科%宋慧锋%许明火
趙京玉%柴傢科%宋慧鋒%許明火
조경옥%시가과%송혜봉%허명화
透明质酸%皮肤移植%生物力学
透明質痠%皮膚移植%生物力學
투명질산%피부이식%생물역학
Hyaluronic acid%Skin transplantation%Biomechanics
目的 探讨透明质酸(HA)对复合移植皮肤组织生物力学顺应性的影响.方法 选用10只日本大耳兔作为实验动物,每只大耳兔背部对称分4个区域,并随机分为4组.其中A、B、C组造成全层皮肤缺损创面,D组为正常皮肤,分别进行如下处理,A组:HA+异种(猪)去细胞真皮基质(PADM)+自体薄厚皮移植；B组:PADM+自体薄厚皮移植；C组:自体薄厚皮回植；D组:不作处理.于术后第70天取创面组织标本测量挛缩率,用Instron机对各组创面组织进行顺应性测试；用Western印迹检测各组复合移植组织中Ⅰ型、Ⅲ型胶原蛋白表达.结果 A、B、C组挛缩率分别为10.2％±0.6％、36.6％±0.8％、32.7％±1.4％,差异有统计学意义(P=0.000)；在相同的拉伸长度下,A组与D组应力值分别为(83±8)与(81±5)kPa,差异无统计学意义(P=0.552)；B组应力值为(215±9)kPa,与D组差异有统计学意义(P=0.000)；C组应力值为(106±7)kPa,与B、D组比较差异均有统计学意义(均P=0.000).A组局部Ⅰ型、Ⅲ型胶原蛋白表达高于B组(均P=0.000),而Ⅰ/Ⅲ型胶原比例低于B组.结论 外用HA可提高并优化局部Ⅰ型、Ⅲ型胶原蛋白表达,提高复合移植皮肤组织的顺应性.
目的 探討透明質痠(HA)對複閤移植皮膚組織生物力學順應性的影響.方法 選用10隻日本大耳兔作為實驗動物,每隻大耳兔揹部對稱分4箇區域,併隨機分為4組.其中A、B、C組造成全層皮膚缺損創麵,D組為正常皮膚,分彆進行如下處理,A組:HA+異種(豬)去細胞真皮基質(PADM)+自體薄厚皮移植；B組:PADM+自體薄厚皮移植；C組:自體薄厚皮迴植；D組:不作處理.于術後第70天取創麵組織標本測量攣縮率,用Instron機對各組創麵組織進行順應性測試；用Western印跡檢測各組複閤移植組織中Ⅰ型、Ⅲ型膠原蛋白錶達.結果 A、B、C組攣縮率分彆為10.2％±0.6％、36.6％±0.8％、32.7％±1.4％,差異有統計學意義(P=0.000)；在相同的拉伸長度下,A組與D組應力值分彆為(83±8)與(81±5)kPa,差異無統計學意義(P=0.552)；B組應力值為(215±9)kPa,與D組差異有統計學意義(P=0.000)；C組應力值為(106±7)kPa,與B、D組比較差異均有統計學意義(均P=0.000).A組跼部Ⅰ型、Ⅲ型膠原蛋白錶達高于B組(均P=0.000),而Ⅰ/Ⅲ型膠原比例低于B組.結論 外用HA可提高併優化跼部Ⅰ型、Ⅲ型膠原蛋白錶達,提高複閤移植皮膚組織的順應性.
목적 탐토투명질산(HA)대복합이식피부조직생물역학순응성적영향.방법 선용10지일본대이토작위실험동물,매지대이토배부대칭분4개구역,병수궤분위4조.기중A、B、C조조성전층피부결손창면,D조위정상피부,분별진행여하처리,A조:HA+이충(저)거세포진피기질(PADM)+자체박후피이식；B조:PADM+자체박후피이식；C조:자체박후피회식；D조:불작처리.우술후제70천취창면조직표본측량련축솔,용Instron궤대각조창면조직진행순응성측시；용Western인적검측각조복합이식조직중Ⅰ형、Ⅲ형효원단백표체.결과 A、B、C조련축솔분별위10.2％±0.6％、36.6％±0.8％、32.7％±1.4％,차이유통계학의의(P=0.000)；재상동적랍신장도하,A조여D조응력치분별위(83±8)여(81±5)kPa,차이무통계학의의(P=0.552)；B조응력치위(215±9)kPa,여D조차이유통계학의의(P=0.000)；C조응력치위(106±7)kPa,여B、D조비교차이균유통계학의의(균P=0.000).A조국부Ⅰ형、Ⅲ형효원단백표체고우B조(균P=0.000),이Ⅰ/Ⅲ형효원비례저우B조.결론 외용HA가제고병우화국부Ⅰ형、Ⅲ형효원단백표체,제고복합이식피부조직적순응성.
Objective To iexplore the effect on biomechanical compliance after an addition of hyaluronic acid(HA)in transplantation of porcine acellular dermal matrix (PADM) with skin autograft.Methods Ten Japanese white rabbits were used as experimental animal,the dorsa of rabbits was symmetrically divided into four area,and then grouping was randomized.Full-thickness skin defects were created in Group A to Group C,and Group D was blank group with normal skin.The following procedures were performed:Group A:implanted with HA + PADM + thin skin autografts,GroupB:implanted with PADM + thin skin autografts and Group C:skin autografts,except for Group D. The wound tissue specimens were harvested at 70 days postoperatively,and they were recorded contracture rates of the grafts and the biomechanical compliance by Instron biomechanics tensiometer.The expression of collagens Ⅰ and Ⅲ in dermal fibroblasts of each group was determined by Western blot.Results On postoperation day 70,the grafts contracture rates in Group A to Group C were 10.2％±0.6％,36.6％±0.8％,32.7％±1.4％ respectively,there were notable significant difference among three groups ( P =0.000 ).Under the same tension,there was no significant difference in strain between Groups A and D ( (83±8) vs (81±5)kPa,P=0.552).Some significant difference in strain existed between Groups B and D ((215±9) vs (81±5 ) kPa,P=0.000).The strain of Group C ( ( 106±7 ) kPa) was between Groups B and D,and significant strain difference existed among them (P=0.000).The expressions of collagens Ⅰ and Ⅲ in Group A were higher than those in Group B,while the ratio of collagens Ⅰ to Ⅲ in Group A was lower than that in Group B.Conclusion Topical HA may be used to increase the expressions of type Ⅰ and Ⅲ collagen,decrease the ratio of collagen type Ⅰ / Ⅲ,and enhance the biomechanics performances of transplanted skin.
