中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2011年
7期
508-512
,共5页
邓秋华%邱源%莫明聪%张鑫%熊信国%江梅%何萍%温丹萍%郑贵星%何建行
鄧鞦華%邱源%莫明聰%張鑫%熊信國%江梅%何萍%溫丹萍%鄭貴星%何建行
산추화%구원%막명총%장흠%웅신국%강매%하평%온단평%정귀성%하건행
癌,非小细胞肺%表皮生长因子受体%基因拷贝数%核苷酸切除修复交叉互补基因1%乳腺癌易感基因1
癌,非小細胞肺%錶皮生長因子受體%基因拷貝數%覈苷痠切除脩複交扠互補基因1%乳腺癌易感基因1
암,비소세포폐%표피생장인자수체%기인고패수%핵감산절제수복교차호보기인1%유선암역감기인1
Carcinoma,non-small cell lung%Epidermal growth receptor%Gene copy numbers%Excision repair cross complementation group 1%Breast cancer susceptibility gene-1
目的 探讨表皮生长因子受体(EGFR)基因拷贝数与铂类耐药相关基因核苷酸切除修复交叉互补基因1(ERCC1)和乳腺癌易感基因1(BRCA1)在非小细胞肺癌(NSCLC)中的表达情况,以及三者之间的相关性.方法 用荧光原位杂交(FISH)技术检测EGFR基因的扩增情况,应用免疫组化SP法检测132例NSCLC患者标本ERCC1和BRCA1蛋白的表达,并进一步分析EGFR基因拷贝数、ERCC1和BRCA1的蛋白表达与患者临床病理特征的关系.结果 EGFR基因FISH阳性率为24.1% (40/166).女性的扩增阳性率高于男性(31.9%和18.6%,P=0.048);无吸烟史的患者扩增阳性率明显高于有吸烟史的患者(32.8%和16.7%,P=0.045);基因拷贝数增加与患者的年龄、临床分期、病理类型及有无淋巴结或远处转移均无关.ERCC1蛋白表达阳性率为45.5% (60/132),在不同的病理类型分布比较,差异有统计学意义(P=0.046),与患者的性别、年龄、临床分期、有无淋巴结或远处转移及吸烟状况均无关.BRCA1蛋白表达阳性率为35.1%(46/131),与患者的性别、年龄、临床分期、病理类型、淋巴结或远处转移及吸烟状况无关.ERCC1与BRCA1的蛋白表达呈中等相关(r=0.449,P＜0.001),与EGFR扩增无相关性(P=0.785);BRCA1表达与EGFR基因拷贝数状态无相关性(P=0.143).结论 在肺癌的个体化治疗中,检测EGFR基因拷贝数、ERCC1和BRCA1的表达对靶向治疗和铂类药物化疗的选择具有重要的临床指导意义.
目的 探討錶皮生長因子受體(EGFR)基因拷貝數與鉑類耐藥相關基因覈苷痠切除脩複交扠互補基因1(ERCC1)和乳腺癌易感基因1(BRCA1)在非小細胞肺癌(NSCLC)中的錶達情況,以及三者之間的相關性.方法 用熒光原位雜交(FISH)技術檢測EGFR基因的擴增情況,應用免疫組化SP法檢測132例NSCLC患者標本ERCC1和BRCA1蛋白的錶達,併進一步分析EGFR基因拷貝數、ERCC1和BRCA1的蛋白錶達與患者臨床病理特徵的關繫.結果 EGFR基因FISH暘性率為24.1% (40/166).女性的擴增暘性率高于男性(31.9%和18.6%,P=0.048);無吸煙史的患者擴增暘性率明顯高于有吸煙史的患者(32.8%和16.7%,P=0.045);基因拷貝數增加與患者的年齡、臨床分期、病理類型及有無淋巴結或遠處轉移均無關.ERCC1蛋白錶達暘性率為45.5% (60/132),在不同的病理類型分佈比較,差異有統計學意義(P=0.046),與患者的性彆、年齡、臨床分期、有無淋巴結或遠處轉移及吸煙狀況均無關.BRCA1蛋白錶達暘性率為35.1%(46/131),與患者的性彆、年齡、臨床分期、病理類型、淋巴結或遠處轉移及吸煙狀況無關.ERCC1與BRCA1的蛋白錶達呈中等相關(r=0.449,P＜0.001),與EGFR擴增無相關性(P=0.785);BRCA1錶達與EGFR基因拷貝數狀態無相關性(P=0.143).結論 在肺癌的箇體化治療中,檢測EGFR基因拷貝數、ERCC1和BRCA1的錶達對靶嚮治療和鉑類藥物化療的選擇具有重要的臨床指導意義.
목적 탐토표피생장인자수체(EGFR)기인고패수여박류내약상관기인핵감산절제수복교차호보기인1(ERCC1)화유선암역감기인1(BRCA1)재비소세포폐암(NSCLC)중적표체정황,이급삼자지간적상관성.방법 용형광원위잡교(FISH)기술검측EGFR기인적확증정황,응용면역조화SP법검측132례NSCLC환자표본ERCC1화BRCA1단백적표체,병진일보분석EGFR기인고패수、ERCC1화BRCA1적단백표체여환자림상병리특정적관계.결과 EGFR기인FISH양성솔위24.1% (40/166).녀성적확증양성솔고우남성(31.9%화18.6%,P=0.048);무흡연사적환자확증양성솔명현고우유흡연사적환자(32.8%화16.7%,P=0.045);기인고패수증가여환자적년령、림상분기、병리류형급유무림파결혹원처전이균무관.ERCC1단백표체양성솔위45.5% (60/132),재불동적병리류형분포비교,차이유통계학의의(P=0.046),여환자적성별、년령、림상분기、유무림파결혹원처전이급흡연상황균무관.BRCA1단백표체양성솔위35.1%(46/131),여환자적성별、년령、림상분기、병리류형、림파결혹원처전이급흡연상황무관.ERCC1여BRCA1적단백표체정중등상관(r=0.449,P＜0.001),여EGFR확증무상관성(P=0.785);BRCA1표체여EGFR기인고패수상태무상관성(P=0.143).결론 재폐암적개체화치료중,검측EGFR기인고패수、ERCC1화BRCA1적표체대파향치료화박류약물화료적선택구유중요적림상지도의의.
Objective To evaluate the expression of epidermal growth factor receptor (EGFR) gene copy number and the expression of ERCC1 and BRCA1 proteins in patients with non-small-cell lung cancer (NSCLC) and the correlation between them. Methods The status of EGFR gene copy number was determined by in situ hybridization (FISH), and the expression of ERCC1 and BRCC1 proteins was examined by immunohistochemistry (IHC). The relationship of EGFR gene copy number with the expression of ERCC1 and BRCA1 and the clinical pathologic features were analyzed. Results FISH-positive EGFR expression was identified in 40 of 166 samples (24.1%). More FISH-positive EGFR in the female than male patients (31.9% vs. 18.6%, P=0.048), and non-smoker than smoker (32.8% vs. 16.7%, P=0.045). FISH-positive EGFR was not associated with age, pathological type, clinical stage and metestasis status (P＞0.05). The expression of ERCC1 protein was identified in 60 of 132 samples (45.5%). The expression of ERCC1 protein varied significantly in tumors of different pathological types (P=0.046), but not associated with age, gender, clinical stage, metestatic status and smoking status (P＞0.05). The expression of BRCA1 protein was identified in 46 of 131 samples (35.1%). The expression of BRCA1 was not associated with age gender, pathological type, clinical stage, metestatic ststus and smoking status (P＞0.05). There was a moderate correlation between the expressions of ERCC1 and BRCA1 (r=0.449, P＜0.001), but EGFR gene copy number was not correlated with the expression of ERCC1 or BRCA1 protein. Conclusions FISH-positive EGFR expression is associated with gender and smoking status, but not correlated with the expression of ERCC1 and BRCA1 proteins. There is a moderate correlation between the expressions of ERCC1 and BRCA1.
