CAJ | 학술논문

通过传统的敲片、Giemsa染色的方法制片对中国黑腹果蝇种组(Drosophila melanogaster species group)8个种亚组40种果蝇的染色体进行了分析,共发现18种核型,即A、A″、C、C′、C″、C″′、C″″、D、D′、D″、E、E′、E"、F、F′、G、H和I,其中A、A″、C″′、C″″、D″和F′为新发现的核型.8个种亚组的基本核型分别是:嗜凤梨果蝇种亚组(D.ananassae subgroup)的核型为F、F′、G和H型;牵牛花果蝇种亚组(D.elegans subgroup)的核型为A和A″型;细针果蝇种亚组(D.eugracilis subgroup)的核型为C型;嗜榕果蝇种亚组(D.ficusphila subgroup)的核型为C′型;黑腹果蝇种亚组(D.melanogaster subgroup)的核型为C和C′型;山果蝇种亚组(D.montium subgroup)的核型为C、C′、C″、D、D′、D″、E、E′、E"和I型;铃木氏果蝇种亚组(D.suzukii subgroup)的核型为C′′′和C″″型;高桥氏果蝇种亚组(D.takahashii subgroup)的核型为C、C′′′和C″″型.透明翅果蝇(D.lucipennis)雌性核型2n=8,雄性核型2n=7,雄性Ⅳ号染色体为染色体单体.此外还发现,吉川氏果蝇(D.kikkawai)、林氏果蝇(D.lini)、奥尼氏果蝇(D.ogumai)、拟嗜凤梨果蝇(D.pseudoananassae)和叔白颜果蝇(D.triauraria)5种果蝇有B染色体.本文确定了D.sp.like elegans、D.sp.like nyinyii、D.sp.liketrapezifrons1、D.sp.like takahashii、D.sp.like trapezifrons2和D.sp.like auraria等6个未描述种的核型和1个新记录种吉里果蝇(D.giriensis)的核型.本研究证明了在黑腹果蝇种组内、亚组内、种内和单雌系内的核型多样性,为果蝇遗传和进化提供了进一步的细胞学证据.
통과전통적고편、Giemsa염색적방법제편대중국흑복과승충조(Drosophila melanogaster species group)8개충아조40충과승적염색체진행료분석,공발현18충핵형,즉A、A″、C、C′、C″、C″′、C″″、D、D′、D″、E、E′、E"、F、F′、G、H화I,기중A、A″、C″′、C″″、D″화F′위신발현적핵형.8개충아조적기본핵형분별시:기봉리과승충아조(D.ananassae subgroup)적핵형위F、F′、G화H형;견우화과승충아조(D.elegans subgroup)적핵형위A화A″형;세침과승충아조(D.eugracilis subgroup)적핵형위C형;기용과승충아조(D.ficusphila subgroup)적핵형위C′형;흑복과승충아조(D.melanogaster subgroup)적핵형위C화C′형;산과승충아조(D.montium subgroup)적핵형위C、C′、C″、D、D′、D″、E、E′、E"화I형;령목씨과승충아조(D.suzukii subgroup)적핵형위C′′′화C″″형;고교씨과승충아조(D.takahashii subgroup)적핵형위C、C′′′화C″″형.투명시과승(D.lucipennis)자성핵형2n=8,웅성핵형2n=7,웅성Ⅳ호염색체위염색체단체.차외환발현,길천씨과승(D.kikkawai)、림씨과승(D.lini)、오니씨과승(D.ogumai)、의기봉리과승(D.pseudoananassae)화숙백안과승(D.triauraria)5충과승유B염색체.본문학정료D.sp.like elegans、D.sp.like nyinyii、D.sp.liketrapezifrons1、D.sp.like takahashii、D.sp.like trapezifrons2화D.sp.like auraria등6개미묘술충적핵형화1개신기록충길리과승(D.giriensis)적핵형.본연구증명료재흑복과승충조내、아조내、충내화단자계내적핵형다양성,위과승유전화진화제공료진일보적세포학증거.