CAJ | 학술논문

目的:评价Bevacizumab抑制大鼠角膜新生血管的效果.方法:碱烧伤诱导角膜新生血管模型,对20只wistar大鼠40眼随机平均分4组.角膜碱烧伤后分别给予结膜下注射药物溶剂和不同剂量Bevacizumab.比较并计算角膜新生血管生长面积.16d后角膜标本行HE检验和免疫组织化学检测VEGF的表达.结果:各用药组与对照组新生血管面积比较差异有统计学意义;治疗组间新生血管面积比较差异没有统计学意义.组织学发现各药物治疗组炎性细胞浸润、新生血管形成均明显轻于对照组.新生血管对照组VEGF染色明显增强,药物注射组表达明显减弱.结论:结膜下注射一定浓度的Bevacizumab对大鼠角膜碱烧伤形成的新生血管有抑制作用.
목적:평개Bevacizumab억제대서각막신생혈관적효과.방법:감소상유도각막신생혈관모형,대20지wistar대서40안수궤평균분4조.각막감소상후분별급여결막하주사약물용제화불동제량Bevacizumab.비교병계산각막신생혈관생장면적.16d후각막표본행HE검험화면역조직화학검측VEGF적표체.결과:각용약조여대조조신생혈관면적비교차이유통계학의의;치료조간신생혈관면적비교차이몰유통계학의의.조직학발현각약물치료조염성세포침윤、신생혈관형성균명현경우대조조.신생혈관대조조VEGF염색명현증강,약물주사조표체명현감약.결론:결막하주사일정농도적Bevacizumab대대서각막감소상형성적신생혈관유억제작용.
AIM To evaluate the anti-angiogenesis action of Bevacizumab on corneal neovascularization(CNV) in rats induced by alkali burns.·METHODS: 20 Health Wistar rats, aging from 6 to 8 weeks and weighting from 170g to 190g from 170g to 190g were prepared for CNV animal models. Both corneas of each animal in experimental were cauterized with alkali, then all rats were randomly divided into four groups (each group have 5 rats and 10 corneas), the both corneas of each rats were received subconjunctival Bevacizumab in different dosage (group 2, 0.5rag; group 3, 1.0mg; group 4, 2.0mg)and the group 1 received carrier solution. The occurrence and development of CNV were observed by slit-lamp microscope, and length and area of CNV were calculated. All rats were followeded up 16 days after alkali burns. The 40 corneas were taken for histopathological examination. Vascular endothelial growth factor (VEGF) were detected in all rat corneas by immunohistochemistry method. ·RESULTS: In the bevacizumabotreated eyes, the vascular area was lower than in the control eyes. The treated group was statistical differences compared with the control group; when vascular area were compared between the treated groups, no statistical differences were observed. The histopathological findings showed that the inflammation cells and the neovascularity in each treated group were significantly fewer than that in the control group. The expression of VEGF markedly increased in CNV control group compared with bevacizumab-injected group. ·CONCLUSION: Subconjunctival application of a certain concentrations Bevacizumab could inhibit angiogenesis in rats corneas induced by alkali burns.