中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2012年
7期
510-513
,共4页
蔡仁梅%瓮占平%王云英%李燕婷%纪向虹
蔡仁梅%甕佔平%王雲英%李燕婷%紀嚮虹
채인매%옹점평%왕운영%리연정%기향홍
先兆子痫%神经生长因子类%S100蛋白质类%细胞凋亡%逆转录聚合酶链反应
先兆子癇%神經生長因子類%S100蛋白質類%細胞凋亡%逆轉錄聚閤酶鏈反應
선조자간%신경생장인자류%S100단백질류%세포조망%역전록취합매련반응
Pre-eclampsia%Nerve growth factors%S100 Proteins%Apoptosis%Reverse transcriptase polymerase chain reaction
目的 通过检测S100B蛋白在早发型及晚发型子痫前期胎盘组织滋养细胞中的表达,探讨S100B蛋白表达与早发型及晚发型子痫前期发病的关系.方法 选择2010年10月-2011年9月在青岛市市立医院产科以剖宫产术分娩的子痫前期产妇60例,其中早发型子痫前期产妇30例(早发型组,发病孕周＜ 34周),晚发型子痫前期产妇30例(晚发型组,发病孕周≥34周),随机选择同期健康足月妊娠(因骨盆异常、臀位、巨大儿、社会因素等原因)行剖宫产术分娩的产妇30例为健康对照组.采用逆转录(RT) -PCR技术以及免疫组化方法分别检测各组产妇胎盘滋养细胞中S100BmRNA及蛋白的表达.结果 (1)S100B mRNA在各组胎盘滋养细胞中均有表达,其中在早发型组为0.73±0.11,晚发型组为0.64±0.10,健康对照组为0.58±0.08；其早发型组与晚发型组及健康对照组比较,差异有统计学意义(P＜0.05)；晚发型组与健康对照组比较,差异无显著意义(P＞0.05).(2)S100B蛋白在各组胎盘滋养细胞中均有表达,主要表达于胎盘滋养细胞的细胞膜、细胞质内,呈棕黄色或棕褐色颗粒.S100B蛋白在早发型组的阳性表达率为100％( 30/30),在晚发型组的阳性表达率为70％(21/30),健康对照组的阳性表达率为63％ (19/30),早发型组与晚发型组及健康对照组比较,差异有统计学意义(P＜0.05)；晚发型组与健康对照组比较,差异无统计学意义(P＞0.05).结论 早发型与晚发型子痫前期可能存在不同的发病机制,S100B蛋白参与了早发型子痫前期的发病.
目的 通過檢測S100B蛋白在早髮型及晚髮型子癇前期胎盤組織滋養細胞中的錶達,探討S100B蛋白錶達與早髮型及晚髮型子癇前期髮病的關繫.方法 選擇2010年10月-2011年9月在青島市市立醫院產科以剖宮產術分娩的子癇前期產婦60例,其中早髮型子癇前期產婦30例(早髮型組,髮病孕週＜ 34週),晚髮型子癇前期產婦30例(晚髮型組,髮病孕週≥34週),隨機選擇同期健康足月妊娠(因骨盆異常、臀位、巨大兒、社會因素等原因)行剖宮產術分娩的產婦30例為健康對照組.採用逆轉錄(RT) -PCR技術以及免疫組化方法分彆檢測各組產婦胎盤滋養細胞中S100BmRNA及蛋白的錶達.結果 (1)S100B mRNA在各組胎盤滋養細胞中均有錶達,其中在早髮型組為0.73±0.11,晚髮型組為0.64±0.10,健康對照組為0.58±0.08；其早髮型組與晚髮型組及健康對照組比較,差異有統計學意義(P＜0.05)；晚髮型組與健康對照組比較,差異無顯著意義(P＞0.05).(2)S100B蛋白在各組胎盤滋養細胞中均有錶達,主要錶達于胎盤滋養細胞的細胞膜、細胞質內,呈棕黃色或棕褐色顆粒.S100B蛋白在早髮型組的暘性錶達率為100％( 30/30),在晚髮型組的暘性錶達率為70％(21/30),健康對照組的暘性錶達率為63％ (19/30),早髮型組與晚髮型組及健康對照組比較,差異有統計學意義(P＜0.05)；晚髮型組與健康對照組比較,差異無統計學意義(P＞0.05).結論 早髮型與晚髮型子癇前期可能存在不同的髮病機製,S100B蛋白參與瞭早髮型子癇前期的髮病.
목적 통과검측S100B단백재조발형급만발형자간전기태반조직자양세포중적표체,탐토S100B단백표체여조발형급만발형자간전기발병적관계.방법 선택2010년10월-2011년9월재청도시시립의원산과이부궁산술분면적자간전기산부60례,기중조발형자간전기산부30례(조발형조,발병잉주＜ 34주),만발형자간전기산부30례(만발형조,발병잉주≥34주),수궤선택동기건강족월임신(인골분이상、둔위、거대인、사회인소등원인)행부궁산술분면적산부30례위건강대조조.채용역전록(RT) -PCR기술이급면역조화방법분별검측각조산부태반자양세포중S100BmRNA급단백적표체.결과 (1)S100B mRNA재각조태반자양세포중균유표체,기중재조발형조위0.73±0.11,만발형조위0.64±0.10,건강대조조위0.58±0.08；기조발형조여만발형조급건강대조조비교,차이유통계학의의(P＜0.05)；만발형조여건강대조조비교,차이무현저의의(P＞0.05).(2)S100B단백재각조태반자양세포중균유표체,주요표체우태반자양세포적세포막、세포질내,정종황색혹종갈색과립.S100B단백재조발형조적양성표체솔위100％( 30/30),재만발형조적양성표체솔위70％(21/30),건강대조조적양성표체솔위63％ (19/30),조발형조여만발형조급건강대조조비교,차이유통계학의의(P＜0.05)；만발형조여건강대조조비교,차이무통계학의의(P＞0.05).결론 조발형여만발형자간전기가능존재불동적발병궤제,S100B단백삼여료조발형자간전기적발병.
Objective To investigate the relationship of S100B protein expression and the pathogenesis of early-onset and late-onset preeclampsia.Methods Sixty patients with preeclampsia who received caesarean section at Qingdao Municipal Hospital from October 2010 to September 2011 were enrolled in this study.Thirty cases were early-onset preeclampsia( referred as early-onset preeclampsia group,＜ 34 weeks),and the other 30 cases were late-onset preeclampsia (referred as late-onset preeclampsia group,≥34 weeks).Thirty women who received caesarean section because of pelvic structural deformities,breech presentation,macrosomia and social factors were included as the control group.The expression of S100B mRNA in the placenta was detected by reverse transcription ( RT)-PCR.The expression of S100B protein in the placenta was detected by immunohistochemistry.Results ( 1 ) S100B mRNA was expressed in the trophoblasts of preeclampsia and control groups.The expression of S100B mRNA in early-onset preeclampsia group (0.73 ±0.11 ) was significantly higher than the control group (0.58 ±0.08) and lateonset preeclampsia group (0.64 ±0.10,P ＜0.05 ).There was no significant difference between late-onset preeclampsia group and the control group ( P ＞ 0.05 ).(2) S100B protein was expressed in the plasma membrane and cytoplasm of the trophoblasts,correlated positively with the brownish yellow and brown particles inside the cells.It was expressed in all the three groups.Immunohistochemistry revealed that the expression of S100B protein in the placenta of early-onset preeclampsia group was 100％ (30/30),significantly higher than those of late-onset preeclampsia group and the control group,in which the positive rate were 70％ (21/30) and 63％ (19/30) respectively (P ＜0.05).There was no difference between late onset preeclampsia group and the control group (P ＞0.05).Conclusion Early-onset and late-onset preeclampsia may have different etiology and pathogenesis.S100B may be a factor in the pathogenesis of early-onset preeclampsia.
