CAJ | 학술논문

目的了解我国自身抗体检测的现状,进一步规范和提高我国自身抗体检测的水平.方法以自愿报名的方法确定参与单位.质量控制项同包括抗核抗体(ANA)、抗双链DNA(dsDNA)抗体、抗可提取核抗原(ENA)抗体、抗线粒体抗体(AMA)/抗平滑肌抗体(ASMA)和抗环瓜氨酸肽(CCP)抗体5项,共15份标本.标本发放及结果分析采用双盲操作.结果共102家医院或科室参加质量控制活动.ANA、抗dsDNA抗体、AMA、ASMA和抗CCP抗体的正确率分别为70%、88%、93%、85%和79%;抗RNP抗体、抗Sm抗体、抗SSA抗体、抗SSB抗体和抗Scl-70 正确率分别为88%、77%、92%、93%和87%.结论我国自身抗体检测情况与前4次质鼍控制相比,参与质量控制的医院和检测的项目逐年增多,ANA、抗dsDNA抗体、抗ENA抗体和AMA检测水平降低,而ASMA和抗CCP抗体检测水平有所提高.
목적 료해아국자신항체검측적현상,진일보규범화제고아국자신항체검측적수평.방법 이자원보명적방법학정삼여단위.질량공제항동포괄항핵항체(ANA)、항쌍련DNA(dsDNA)항체、항가제취핵항원(ENA)항체、항선립체항체(AMA)/항평활기항체(ASMA)화항배과안산태(CCP)항체5항,공15빈표본.표본발방급결과분석채용쌍맹조작.결과 공102가의원혹과실삼가질량공제활동.ANA、항dsDNA항체、AMA、ASMA화항CCP항체적정학솔분별위70%、88%、93%、85%화79%;항RNP항체、항Sm항체、항SSA항체、항SSB항체화항Scl-70 정학솔분별위88%、77%、92%、93%화87%.결론 아국자신항체검측정황여전4차질타공제상비,삼여질량공제적의원화검측적항목축년증다,ANA、항dsDNA항체、항ENA항체화AMA검측수평강저,이ASMA화항CCP항체검측수평유소제고.
Objective To consecutively understand the national clinical testing status and to reassure the quality-control of autoantibody detection. Methods Letter or telephone notification were conducted to the participating hospitals or departments. Autoantibodies for quality control survey included anti-nuclear anti-body (ANA), anti-double-stranded DNA (anti-dsDNA), anti-extractable nuclear antigens (A-ENA), anti-mitochondria antibody (AMA)/anti-smooth muscle antibody (ASMA), and anti-citrulline antibody (anti-CCP). Each had 3 control samples, and altogether 15 samples for testing. Sample distribution and data analysis were double-blinded. Results One hundred and two hospitals/departments participated in the national quality-control survey. The accurate rate for this survey was 70%, 88%, 93%, 85%, 79% respectively for ANA, anti-dsDNA, AMA, ASMA and anti-CCP. Anti-ENAs were further divided into anti-RNP, Sm, SSA, SSB and Scl-70 subgroups, and the accurate rate was 88%, 77%, 92%, 93% and 87% respectively. Conclu-sion Compared to the previous 4 national surveys, the accurate rates of ANA, anti-dsDNA, anti-ENAs, AMA in our country's autoantibody testing is improved, and the detection rate of ASMA and anti-CCP antibody is also increased. More hospitals and testing items can test these autoantibodies, which implies that autoantibody testing status has been improving in our country.