中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2011年
6期
769-771,775
,共4页
糖尿病肾病/代谢%DNA损伤%基质金属蛋白酶2/代谢%蛋白水解产物/代谢
糖尿病腎病/代謝%DNA損傷%基質金屬蛋白酶2/代謝%蛋白水解產物/代謝
당뇨병신병/대사%DNA손상%기질금속단백매2/대사%단백수해산물/대사
Diabetic nephropathies/ME%DNA damage%Matrix metalloproteinase 2/ME%Protein hydrolysates/ME
目的 检测糖尿病肾病患者血清蛋白氧化损伤指标的变化,探讨蛋白氧化损伤在糖尿病肾病发病中的可能机制.方法 应用改良后的Witko-Sarsat法和2,4-二硝基苯肼法检测血清晚期氧化蛋白产物(AOPP)水平、蛋白质羰基(PCO)含量,应用ELISA及酶谱法分别检测血清基质金属蛋白酶-2(MMP-2)的表达水平和活性.结果 DN1组、DN2组AOPP水平明显高于DM组(78.23±19.30 vs 61.25±12.13、101.59±30.22 vs 61.25±12.13,F＝41.988,P＜0.01),DN1组、DN2组PCO含量亦明显高于DM组(0.84±0.03 vs 0.66±0.02、1.05±0.05 vs 0.66±0.02,F＝205.763,P＜0.01),AOPP、PCO与血清MMP-2的表达水平(r＝0.460,0.480,P＜0.05)和活性(r＝0.385,0.560,P＜0.05)均呈正相关,多元逐步回归分析表明AOPP、PCO为血清MMP-2表达水平(P＜0.05,P＜0.01)和活性(P＜0.01,P＜0.05)的主要影响因素.结论 推测蛋白氧化损伤通过改变MMP-2的表达和活性,影响肾脏基质代谢.
目的 檢測糖尿病腎病患者血清蛋白氧化損傷指標的變化,探討蛋白氧化損傷在糖尿病腎病髮病中的可能機製.方法 應用改良後的Witko-Sarsat法和2,4-二硝基苯肼法檢測血清晚期氧化蛋白產物(AOPP)水平、蛋白質羰基(PCO)含量,應用ELISA及酶譜法分彆檢測血清基質金屬蛋白酶-2(MMP-2)的錶達水平和活性.結果 DN1組、DN2組AOPP水平明顯高于DM組(78.23±19.30 vs 61.25±12.13、101.59±30.22 vs 61.25±12.13,F＝41.988,P＜0.01),DN1組、DN2組PCO含量亦明顯高于DM組(0.84±0.03 vs 0.66±0.02、1.05±0.05 vs 0.66±0.02,F＝205.763,P＜0.01),AOPP、PCO與血清MMP-2的錶達水平(r＝0.460,0.480,P＜0.05)和活性(r＝0.385,0.560,P＜0.05)均呈正相關,多元逐步迴歸分析錶明AOPP、PCO為血清MMP-2錶達水平(P＜0.05,P＜0.01)和活性(P＜0.01,P＜0.05)的主要影響因素.結論 推測蛋白氧化損傷通過改變MMP-2的錶達和活性,影響腎髒基質代謝.
목적 검측당뇨병신병환자혈청단백양화손상지표적변화,탐토단백양화손상재당뇨병신병발병중적가능궤제.방법 응용개량후적Witko-Sarsat법화2,4-이초기분정법검측혈청만기양화단백산물(AOPP)수평、단백질탄기(PCO)함량,응용ELISA급매보법분별검측혈청기질금속단백매-2(MMP-2)적표체수평화활성.결과 DN1조、DN2조AOPP수평명현고우DM조(78.23±19.30 vs 61.25±12.13、101.59±30.22 vs 61.25±12.13,F＝41.988,P＜0.01),DN1조、DN2조PCO함량역명현고우DM조(0.84±0.03 vs 0.66±0.02、1.05±0.05 vs 0.66±0.02,F＝205.763,P＜0.01),AOPP、PCO여혈청MMP-2적표체수평(r＝0.460,0.480,P＜0.05)화활성(r＝0.385,0.560,P＜0.05)균정정상관,다원축보회귀분석표명AOPP、PCO위혈청MMP-2표체수평(P＜0.05,P＜0.01)화활성(P＜0.01,P＜0.05)적주요영향인소.결론 추측단백양화손상통과개변MMP-2적표체화활성,영향신장기질대사.
Objective Serum indicators of oxidative protein damage (OPD) were analyzed to explore the effect on renal MMP/TIMP system of OPD in diabetic nephropathy. Methods AOPP levels and PCO levels were measured by modified Witko-Sarsat's method and 2, 4-dinitrophenylhydrazine spectrophotometric method, expression of serum MMP-2 were determined by ELISA, and MMP-2 activity were detected by zymography . Results AOPP levels of group DN1, group DN2 were higher than those of group DM(78.23±19.30 vs 61.25±12.13,101.59±30.22 vs 61.25±12.13,F＝41.988,P＜0.01). PCO levels of group DN1 and group DN2 were higher than those of group DM (0.84±0.03 vs 0.66±0.02,1.05±0.05 vs 0.66±0.02,F＝205.763,P＜0.01). Pearson correlation analysis indicated AOPP and PCO were positively correlated with the expression (r＝0.460,0.480,P＜0.05) and activity (r＝0.385,0.560,P＜0.05) of serum MMP-2. Multiple stepwise regression analysis showed that AOPP and PCO were major influential factors of serum MMP-2 expression (P＜0.05,P＜0.01) and activity(P＜0.01,P＜0.05). Conclusions OPD might be involved in the imbalance of renal matrix metabolism, which was correlated with the development of diabetic nephropathy.
