CAJ | 학술논문

目的评价芬太尼和瑞芬太尼对人肺癌A549细胞活力的影响.方法人肺癌A549细胞培养至对数生长期,接种于96孔培养板或75 ml培养瓶中,采用随机数字表法,将其随机分为9组(n=30):不同浓度芬太尼组(F1-4组)芬太尼终浓度分别为0.5、5.0、50.0、500.0 ng/ml,不同浓度瑞芬太尼组(RF1-4组)瑞芬太尼终浓度分别为0.5、50、50.0、500.0 ng/ml,正常对照组(C组)加入等容量的RPMI1640培养基.分别于孵育24、48和72 h时,采用噻唑蓝比色法测定细胞活力；于孵育24h时经AnnexinV- FITC/PI双标记染色后,采用流式细胞仪检测细胞凋亡率,经PI染色后,采用流式细胞仪检测细胞周期分布.结果 C组、F2-4组或RF2～4组孵育72 h时人肺癌A549细胞活力依次降低,孵育24h时S期比例依次降低,G2/M期比例和凋亡率依次升高(P＜0.05).结论芬太尼和瑞芬太尼终浓度≥5 ng/ml时,可浓度依赖性地抑制人肺癌A549细胞活力,其机制可能与诱导细胞凋亡,使细胞周期停滞于G2/M期有关.
목적 평개분태니화서분태니대인폐암A549세포활력적영향.방법 인폐암A549세포배양지대수생장기,접충우96공배양판혹75 ml배양병중,채용수궤수자표법,장기수궤분위9조(n=30):불동농도분태니조(F1-4조)분태니종농도분별위0.5、5.0、50.0、500.0 ng/ml,불동농도서분태니조(RF1-4조)서분태니종농도분별위0.5、50、50.0、500.0 ng/ml,정상대조조(C조)가입등용량적RPMI1640배양기.분별우부육24、48화72 h시,채용새서람비색법측정세포활력；우부육24h시경AnnexinV- FITC/PI쌍표기염색후,채용류식세포의검측세포조망솔,경PI염색후,채용류식세포의검측세포주기분포.결과 C조、F2-4조혹RF2～4조부육72 h시인폐암A549세포활력의차강저,부육24h시S기비례의차강저,G2/M기비례화조망솔의차승고(P＜0.05).결론 분태니화서분태니종농도≥5 ng/ml시,가농도의뢰성지억제인폐암A549세포활력,기궤제가능여유도세포조망,사세포주기정체우G2/M기유관.
Objective To investigate the effects of fentanyl and remifentanil on the viability of human adenocarcinoma cell line A549.Methods Human adenocarcinoma A549 cells cultured in logarithmic growth phase were seeded in 75 ml culture bottles or 96-well plates.After being cultured for 24 h,the cells were randomly divided into 9 groups (n =30 each):4 fentanyl groups (groups F1-4 ),4 remifentanil groups (groups RF1-4 ) and control group (group C).Groups F1-4 were exposed to fentanyl with the final concentrations of 0.5,5.0,50.0 and 500.0 ng/ml respectively.Groups RF1-4 were exposed to remifentanil with the final concentrations of 0.5,5.0,50.0 and 500.0 ng/ml respectively.The viability of the cells was determined by methyl thiazolyl tetrazolium assay after being incubated for 24,48 and 72 h.The cell cycle progression and apoptosis were determined by flow cytometry after being incubated for 24 h.Results Compared with group C,the viability of A549 cells were gradually decreased at 72 h of incubation,the proportion of the cells in S phase was gradually decreased at 24 h of incubation,and the proportion of the cells in G2/M phase and apoptotic rate were gradually increased in groups F2-4 and in groups RF2-4 ( P ＜ 0.05).Conclusion Fentanyl and remifentanil with the final concentration ≥5 ng/ml can inhibit the viability of human adenocarcinoma cell line A549 in a dose-independent manner by inducing cell apoptosis and cell cycle arrest in G2/M phase.