中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2011年
4期
305-309
,共5页
张耀光%王建业%齐若梅%张力青%万奔%魏东%朱生才%何美懿%陈仁涉%曾品玲
張耀光%王建業%齊若梅%張力青%萬奔%魏東%硃生纔%何美懿%陳仁涉%曾品玲
장요광%왕건업%제약매%장력청%만분%위동%주생재%하미의%진인섭%증품령
前列腺增生%前列腺肿瘤%血小板%反应素类
前列腺增生%前列腺腫瘤%血小闆%反應素類
전렬선증생%전렬선종류%혈소판%반응소류
Hyperplasia of prostate%Prostate neoplasms%Blood platelets%Reagins
目的 采用酶联免疫吸附法(ELISA)测定血小板反应素-1(TSP-1),并分析TSP-1对前列腺癌的诊断价值.方法 用ELISA斑点法测定TSP-1,研制了3种具有分离特异抗原功能的试剂,研发了一项具有纯化蛋白能力的技术,解决非特异蛋白的交叉反应问题,建立测定TSP-1的ELISA常规方法.并用该方法测定14例良性前列腺增生和18例前列腺癌患者血清标本的TSP-1.结果 前列腺癌组TSP-1值平均(73.77±12.72)%,前列腺增生组为(121.86±19.47),差异有统计学意义(t=8.44,P＜0.01).TSP-1和前列腺特异性抗原(PSA)对前列腺癌诊断的敏感性和特异性分别为92.9%、88.9%和85.7%、66.7%,差异有统计学意义(均P＜0.01).TSP-1的受试者工作特征曲线(ROC)曲线下面积为0.9663,PSA的ROC曲线下面积为0.7421,差异有统计学意义(P＜0.05).结论 应用ELISA方法测定TSP-1现实可行,TSP-1是一种理想的前列腺癌诊断指标,可能比PSA更能够准确地鉴别良性和恶性前列腺疾病.
目的 採用酶聯免疫吸附法(ELISA)測定血小闆反應素-1(TSP-1),併分析TSP-1對前列腺癌的診斷價值.方法 用ELISA斑點法測定TSP-1,研製瞭3種具有分離特異抗原功能的試劑,研髮瞭一項具有純化蛋白能力的技術,解決非特異蛋白的交扠反應問題,建立測定TSP-1的ELISA常規方法.併用該方法測定14例良性前列腺增生和18例前列腺癌患者血清標本的TSP-1.結果 前列腺癌組TSP-1值平均(73.77±12.72)%,前列腺增生組為(121.86±19.47),差異有統計學意義(t=8.44,P＜0.01).TSP-1和前列腺特異性抗原(PSA)對前列腺癌診斷的敏感性和特異性分彆為92.9%、88.9%和85.7%、66.7%,差異有統計學意義(均P＜0.01).TSP-1的受試者工作特徵麯線(ROC)麯線下麵積為0.9663,PSA的ROC麯線下麵積為0.7421,差異有統計學意義(P＜0.05).結論 應用ELISA方法測定TSP-1現實可行,TSP-1是一種理想的前列腺癌診斷指標,可能比PSA更能夠準確地鑒彆良性和噁性前列腺疾病.
목적 채용매련면역흡부법(ELISA)측정혈소판반응소-1(TSP-1),병분석TSP-1대전렬선암적진단개치.방법 용ELISA반점법측정TSP-1,연제료3충구유분리특이항원공능적시제,연발료일항구유순화단백능력적기술,해결비특이단백적교차반응문제,건립측정TSP-1적ELISA상규방법.병용해방법측정14례량성전렬선증생화18례전렬선암환자혈청표본적TSP-1.결과 전렬선암조TSP-1치평균(73.77±12.72)%,전렬선증생조위(121.86±19.47),차이유통계학의의(t=8.44,P＜0.01).TSP-1화전렬선특이성항원(PSA)대전렬선암진단적민감성화특이성분별위92.9%、88.9%화85.7%、66.7%,차이유통계학의의(균P＜0.01).TSP-1적수시자공작특정곡선(ROC)곡선하면적위0.9663,PSA적ROC곡선하면적위0.7421,차이유통계학의의(P＜0.05).결론 응용ELISA방법측정TSP-1현실가행,TSP-1시일충이상적전렬선암진단지표,가능비PSA경능구준학지감별량성화악성전렬선질병.
Objective To use enzyme-linked immunosorbent assay (ELISA) for measuring thrombospondin-1 (TSP-1),and to analyze its diagnostic value for prostatic carcinoma.Methods The possible difficulties and the way to solve the difficulties with ELISA spot were explored first.Three agents which could segregate idio-antigen and one technique which could depurate proteinum were designed and developed.The non- idio- proteinum cross reaction problems were solved and the routine method to measure TSP-1 with ELISA was set up successfully.The serum TSP-1 was measured in 14 patients with benign prostatic hyperplasia (BPH) and 18 patients with prostatic carcinoma.Results The TSP-1 values were (73.77±12.72)% and (121.86±-19.47)% in prostatic carcinoma group and benign prostatic hyperplasia group,respectively (t= 8.44,P＜0.01).The diagnostic sensitivity and specificity of TSP-1 and prostate specific antigen (PSA) for prostatic cancer were 92.7%,88.9% and 85.7%,66.7%,respectively (P＜0.01).The area under the receiver operating characteristic curve (ROC) of TSP-1 and PSA were 0.9663 and 0.7421 (P＜0.05).Conclusions The determination of TSP-1 with ELISA is feasible.TSP-1 is an ideal diagnostic parameter for prostatic carcinoma and it may distinguish BPH from malignant prostatic disease more exactly than PSA.
