CAJ | 학술논문

目的探讨鲑鱼降钙素的镇痛作用及其对中脑导水管周围灰质(PAG)中降钙素受体表达的影响.方法采用慢性压迫性损伤(CCI)的方法制备神经性疼痛的大鼠模型,热板实验评估热痛阈(TWL),von Frey纤丝测定机械痛阈(MNT),免疫组化法检测PAG中降钙素受体的分布,Western印迹检测降钙素受体蛋白的表达量.40只雄性SD大鼠被平均随机分配至5组:正常组、假手术组、CCI组、CCI+鲑鱼降钙素皮下注射组和CCI+鲑鱼降钙素PAG微量注射组.结果 (1)假手术组大鼠TWL、MNT及PAG中降钙素受体表达较正常组无显著性差异(P＞0.05);(2)CCI组大鼠TWL和MNT较正常组显著降低(P＜0.05),但降钙素受体表达显著高于正常组(P＜0.05);(3)鲑鱼降钙素治疗后CCI大鼠的TWL、MNT和降钙素受体的表达均显著升高(P＜0.05),且PAG注射较皮下注射效果显著(P＜0.05).结论鲑鱼降钙素能够提高CCI大鼠的痛阈并增加PAG中降钙素受体的表达量,而且PAG注射鲑鱼降钙素较皮下注射效果明显.
목적 탐토해어강개소적진통작용급기대중뇌도수관주위회질(PAG)중강개소수체표체적영향.방법 채용만성압박성손상(CCI)적방법제비신경성동통적대서모형,열판실험평고열통역(TWL),von Frey섬사측정궤계통역(MNT),면역조화법검측PAG중강개소수체적분포,Western인적검측강개소수체단백적표체량.40지웅성SD대서피평균수궤분배지5조:정상조、가수술조、CCI조、CCI+해어강개소피하주사조화CCI+해어강개소PAG미량주사조.결과 (1)가수술조대서TWL、MNT급PAG중강개소수체표체교정상조무현저성차이(P＞0.05);(2)CCI조대서TWL화MNT교정상조현저강저(P＜0.05),단강개소수체표체현저고우정상조(P＜0.05);(3)해어강개소치료후CCI대서적TWL、MNT화강개소수체적표체균현저승고(P＜0.05),차PAG주사교피하주사효과현저(P＜0.05).결론 해어강개소능구제고CCI대서적통역병증가PAG중강개소수체적표체량,이차PAG주사해어강개소교피하주사효과명현.
Objective To investigate the analgesic effect of salmon calcitonin(sCT)and its effect on expression of calcitonin receptor(CT-R)in periaqueductal gray(PAG). Methods Rat models of neuropathic pain were prepared by chronic constriction injury(CCI). Thermal withdrawal latency(TWL)and mechanical nociceptive threshold(MNT)were measured using hot plate test and yon Frey monofilaments test. The distribution of CT-R in PAG was detected by immunohistochemical method. CT-R protein was quantitatively determined by western blotting. Fourty male SD rats were randomized into 5 groups: normal group, sham-CCI group, CCI group, CCI plussubcutaneous sCT group, and CCI plus microinjection of sCT into PAG group. Results TWL, MNT, andexpression of CT-R in PAG showed no difference between normal group and sham-CCI group(P＞0. 05). TWL and MNT in CCI group were significantly lower than those in normal group(P＜0.05), and expression of CT-R in CCI group was significantly higher than that in normal group(P＜0.05). TWL, MNT and expression of CT-R in CCI rats increased significantly after sCT therapy(P＜0. 05), and the effect was more marked in PAG injection group than subcutaneous injection group(P＜0.05). Conclusions sCT raises the pain threshold and increase the expression of CT-R in PAG of CCI rats, while PAG injection showed more marked effect than subcutaneous injection.