CAJ | 학술논문

目的探讨基因芯片技术对于颅内感染致病菌快速检测的应用价值.方法选择4种常见致病菌以及6种常见耐药基因的特异DNA序列设计相应的引物与探针,对30例颅内感染患者的脑脊液标本(脑脊液培养阳性12例,阴性18例)进行多重PCR扩增、基因芯片检测,进行菌种鉴定和耐药基因检测,与脑脊液细菌培养及药敏检测结果进行比较.结果 15例标本鉴定出菌种并检出耐药基因；8例未鉴定出菌种但检出细菌及耐药基因；7例未检出细菌及耐药基因.结论基因芯片可以相对灵敏、快速地用于颅内感染患者脑脊液中致病菌检测.
목적 탐토기인심편기술대우로내감염치병균쾌속검측적응용개치.방법 선택4충상견치병균이급6충상견내약기인적특이DNA서렬설계상응적인물여탐침,대30례로내감염환자적뇌척액표본(뇌척액배양양성12례,음성18례)진행다중PCR확증、기인심편검측,진행균충감정화내약기인검측,여뇌척액세균배양급약민검측결과진행비교.결과 15례표본감정출균충병검출내약기인；8례미감정출균충단검출세균급내약기인；7례미검출세균급내약기인.결론 기인심편가이상대령민、쾌속지용우로내감염환자뇌척액중치병균검측.
Objective To assess gene chip application value in detecting pathogenic bacteria in intracranial infection cases.Methods Primers and probes aiming at the specific DNA sequences of 4 kinds of common pathogenic bacteria and 6 kinds of common drug resistance genes (DRGs) were designed and used to identify the bacteria and DRGs among 30 cerebrospinal fluid (CSF) specimens (12 positive,18negative in CSF culture) from patients with intracranial infection using multiplex polymerase chain reaction (mPCR) and gene chip.The results of gene detection were compared with those of CSF culture and drug sensitivity testing.Results Bacteria were identified and DRGs were detected in 15 specimens; DRGs and 16S gene were detected in 8 specimens; neither bacterium nor DRG was detected in 7 specimens.Conclusion Gene chip technique is characterized by its relative sensitivity and rapidity of detecting the pathogenic bacteria in CSF of intraeranial infection cases.