中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2009年
6期
381-384
,共4页
蔡润策%魏丽%狄建忠%于浩泳%包玉倩%贾伟平
蔡潤策%魏麗%狄建忠%于浩泳%包玉倩%賈偉平
채윤책%위려%적건충%우호영%포옥천%가위평
糖尿病,2型%网膜素%脂肪组织%肥胖症
糖尿病,2型%網膜素%脂肪組織%肥胖癥
당뇨병,2형%망막소%지방조직%비반증
Diabetes mellitus,type 2%Adipose tissue%Obesity%Omentin
目的 研究不同糖调节和体重人群脂肪组织网膜素表达差异及其与血清网膜素、体脂参数、糖脂代谢、胰岛素抵抗等的相关性.方法 36例外科择期手术患者分为正常糖调节正常体重(NGR-NW)组,正常糖调节超重/肥胖(NGR-OW/OB)组和2型糖尿病超重/肥胖(T2DM-OW/OB)组.采用实时定量PCR方法 测定腹部皮下和网膜脂肪组织网膜素mRNA水平.用ELISA法检测空腹血清网膜素水平,同时检测空腹血糖(FaG)、口服葡萄糖耐量试验2 h血糖(2h PG)、糖化血红蛋白(HbA1C)、血脂各项指标及-腹胰岛素(FINS),计算体重指数(BMI)和腰臀比值(WHR),并以稳态模型计算胰岛素抵抗指数(HOMA-IR).结果 网膜脂肪组织网膜素mRNA水平显著高于皮下脂防组织[1.52±0.32比0.019±0.006,P<0.01(均以网膜素/β-肌动蛋白来定量)];男性和女性网膜素mRNA水平无差异(1.46±0.31比1.58±0.29,P=0.416);NGR-OW/OB组网膜素mRNA水平显著低于NGR-NW组(1.18±0.29比1.52±0.32,P<0.05),T2DM-OW/OB组网膜素mRNA水平显著低于NGR-NW组和NGR-OW/OB组(分别为0.98±0.37比1.52±0.32和0.98±0.37比1.18±0.29,均P<0.05);相关分析显示,网膜脂肪组织网膜素mRNA水平与HOMA-IR、腰围、WHR、甘油三酯、BMI、FINS呈显著负相关(r=-0.196～-0.283,均P<0.05),与血清网膜素、HDL-C呈显著正相关(r=0.588、0.242,均P<0.05);多元逐步回归分析发现,血清网膜素、HOMA-IR和腰围是网膜素mRNA水平的独立相关因素(相关系数r2=0.145、0.190、0.224,均P<0.05).结论 网膜素在不同个体的差异表达可能参与构成胰岛素抵抗综合征的病理生理基础.
目的 研究不同糖調節和體重人群脂肪組織網膜素錶達差異及其與血清網膜素、體脂參數、糖脂代謝、胰島素牴抗等的相關性.方法 36例外科擇期手術患者分為正常糖調節正常體重(NGR-NW)組,正常糖調節超重/肥胖(NGR-OW/OB)組和2型糖尿病超重/肥胖(T2DM-OW/OB)組.採用實時定量PCR方法 測定腹部皮下和網膜脂肪組織網膜素mRNA水平.用ELISA法檢測空腹血清網膜素水平,同時檢測空腹血糖(FaG)、口服葡萄糖耐量試驗2 h血糖(2h PG)、糖化血紅蛋白(HbA1C)、血脂各項指標及-腹胰島素(FINS),計算體重指數(BMI)和腰臀比值(WHR),併以穩態模型計算胰島素牴抗指數(HOMA-IR).結果 網膜脂肪組織網膜素mRNA水平顯著高于皮下脂防組織[1.52±0.32比0.019±0.006,P<0.01(均以網膜素/β-肌動蛋白來定量)];男性和女性網膜素mRNA水平無差異(1.46±0.31比1.58±0.29,P=0.416);NGR-OW/OB組網膜素mRNA水平顯著低于NGR-NW組(1.18±0.29比1.52±0.32,P<0.05),T2DM-OW/OB組網膜素mRNA水平顯著低于NGR-NW組和NGR-OW/OB組(分彆為0.98±0.37比1.52±0.32和0.98±0.37比1.18±0.29,均P<0.05);相關分析顯示,網膜脂肪組織網膜素mRNA水平與HOMA-IR、腰圍、WHR、甘油三酯、BMI、FINS呈顯著負相關(r=-0.196～-0.283,均P<0.05),與血清網膜素、HDL-C呈顯著正相關(r=0.588、0.242,均P<0.05);多元逐步迴歸分析髮現,血清網膜素、HOMA-IR和腰圍是網膜素mRNA水平的獨立相關因素(相關繫數r2=0.145、0.190、0.224,均P<0.05).結論 網膜素在不同箇體的差異錶達可能參與構成胰島素牴抗綜閤徵的病理生理基礎.
목적 연구불동당조절화체중인군지방조직망막소표체차이급기여혈청망막소、체지삼수、당지대사、이도소저항등적상관성.방법 36예외과택기수술환자분위정상당조절정상체중(NGR-NW)조,정상당조절초중/비반(NGR-OW/OB)조화2형당뇨병초중/비반(T2DM-OW/OB)조.채용실시정량PCR방법 측정복부피하화망막지방조직망막소mRNA수평.용ELISA법검측공복혈청망막소수평,동시검측공복혈당(FaG)、구복포도당내량시험2 h혈당(2h PG)、당화혈홍단백(HbA1C)、혈지각항지표급-복이도소(FINS),계산체중지수(BMI)화요둔비치(WHR),병이은태모형계산이도소저항지수(HOMA-IR).결과 망막지방조직망막소mRNA수평현저고우피하지방조직[1.52±0.32비0.019±0.006,P<0.01(균이망막소/β-기동단백래정량)];남성화녀성망막소mRNA수평무차이(1.46±0.31비1.58±0.29,P=0.416);NGR-OW/OB조망막소mRNA수평현저저우NGR-NW조(1.18±0.29비1.52±0.32,P<0.05),T2DM-OW/OB조망막소mRNA수평현저저우NGR-NW조화NGR-OW/OB조(분별위0.98±0.37비1.52±0.32화0.98±0.37비1.18±0.29,균P<0.05);상관분석현시,망막지방조직망막소mRNA수평여HOMA-IR、요위、WHR、감유삼지、BMI、FINS정현저부상관(r=-0.196～-0.283,균P<0.05),여혈청망막소、HDL-C정현저정상관(r=0.588、0.242,균P<0.05);다원축보회귀분석발현,혈청망막소、HOMA-IR화요위시망막소mRNA수평적독립상관인소(상관계수r2=0.145、0.190、0.224,균P<0.05).결론 망막소재불동개체적차이표체가능삼여구성이도소저항종합정적병리생리기출.
Objeclive To analyze the mRNA expression of omentin mRNA level in subcutaneous and omental adipose tissues of normal,obese,and type 2 diabetic individuals and to investigate the relationship between omentin mRNA expression and serum omentin level,body fat parameters,glucose and Lipid metabolism,and insulin resistance indexes. Methods 36 patients with benign diseases undergoing selective abdominal operation,19 males and 17 females,aged 20 -65,included 12 with normal glucose regulation and normal weight ( NGR-NW group),12 with normal glucose regulation and overweight/obesity (NGR-OW/OB group),and 12 with type 2 diabetes and overweight/obesity (T2DM-OW/OB group). Abdominal subcutaneous and omental adipose tissues were obtained during operation. Real-time quantitative PCR was used to measure the omentin mRNA level . The level of fasting serum omentin was measured by ELISA. Meanwhile blood glucose,HbA1C,hpids and insulin levels were measured. Body weight,BMI and waist hip ratio (WHR) were evaluated and insulin sensitivity was assessed by homeostasis model assessment insulin resistance index (HOMA-IR). Results The omentin mRNA level in omental adipose tissue of the NGR-NW group was ( 1.52 ± 0. 32),significantly higher than that in subcutaneous adipose tissue [ (0.019 ±0.006) ,P <0.01]. The omentin mRNA level of the males was ( 1.46 ± 0.31 ),not statistically different from that of the female [(1.58±0. 29) ,P =0.416]. The omentin mRNA level of the NGR-OW/OB group was (1.18±0.29),significantly lower than that of the NGR-NW group [(1.52±0.32) ,P <0.05],and the omentin mRNA level of the T2DM-OW/OB group was (0.98 ± 0.37 ),both significantly lower than those of the other 2 groups (both P <0.05 ). Partial correlation analysis showed that the omentin mRNA was negatively correlated with HOMA-IR,body weight,WHR,triglyceride,BMI,and fasting insulin,and positively correlated to serum omentin level and HDL-C. Multiple linear regression analysis showed that serum omentin level,HOMA-IR,and body weight were independent variables of omentin. Conclusion Omentin mRNA is highly expressed in omental adipose tissue. The omentin mRNA expression level decreases in the overweight/obese individuals and decreases further when overweight/obesity is combined with type 2 diabetes. Omentin mRNA is positively correlated to serum omentin level,obese indexes,insulin resistance,and lipid metabolism parameters. Decreased omentin gene expression may contribute to the underlying pathophysiology of insulin resistance syndrome.
