背景:羟基磷灰石是一种理想的骨和牙齿的外科医用材料,但其强度低且具有一定的脆性;羟基磷灰石和二氧化锆(3%mol氧化钇稳定的立方相二氧化锆)作为骨替代材料,二者复合后制备生物陶瓷材料对伤口愈合的影响值得研究.目的:观察纳米羟基磷灰石-二氧化锆生物陶瓷刀片材料切割伤口后对其愈合的影响.设计:单一样本观察.单位:哈尔滨医科大学附属第二医院骨二科,哈尔滨工业大学.材料:实验于2006-03/05在哈尔滨医科大学附属第二医院实验动物研究室完成,选用54只4月龄SD大鼠,雌雄不限,体质量120～180 g.实验所使用的纳米羟基磷灰石粉体为自行制备,Ca(NO3)2·4H2O(天津市博迪化工有限公司),(NH4)2HPO4(天津市石英钟厂霸州化工分厂),氨水(绥化市化学试剂厂),无水乙醇(天津化学试剂厂).方法:将纳米羟基磷灰石和二氧化锆粉体经充分研磨后得到纳米羟基磷灰石/二氧化锆复合粉体,以质量比按40%羟基磷灰石+60%二氧化锆比例配制,采用热压烧结技术磨削制成具有一定刃形形状的外形,进行伤口切割实验,即用高压灭菌后的生物陶瓷刃部纵行切开大鼠背部皮肤与皮下组织,切口长为2 cm,再用1#丝线缝合切口.分别在术后第3,7,14天将动物断颈处死,每次取18只,切取切口处皮肤与皮下、肌肉组织进行大体观察,同时进行切片、苏木精-伊红染色,光学显微镜观察伤口愈合情况.主要观察指标:大鼠背部伤口大体及组织学观察结果.结果:纳入大鼠54只,均进入结果分析,无脱失值.术后3 d,大鼠背部伤口边缘可见到表皮撕裂的微细碎屑,切口未愈合,切口边缘有明显的炎性细胞浸润.术后7 d,切口边缘仍可见到表皮撕裂的微细碎屑,但已经开始出现修复反应,碎屑减少,切口变平整,开始愈合,切口皮下组织内有明显的炎性细胞浸润.在高倍光学显微镜下观察,创缘开始愈合,可见到新生的胶原纤维充填于切口间,但胶原纤维较疏松,皮下组织内有大量的浸润的白细胞,为组织修复反应相.术后14 d,伤口创缘间被致密的胶原纤维充填,皮下组织内的白细胞浸润明显减轻,新生的毛细血管内可见到正常的血细胞,切口间新形成的胶原纤维致密坚强,为正常创伤修复与和组织.结论:纳米羟基磷灰石-二氧化锆生物陶瓷材料具有无毒、无过敏等特点,具有良好的生物活性.
揹景:羥基燐灰石是一種理想的骨和牙齒的外科醫用材料,但其彊度低且具有一定的脆性;羥基燐灰石和二氧化鋯(3%mol氧化釔穩定的立方相二氧化鋯)作為骨替代材料,二者複閤後製備生物陶瓷材料對傷口愈閤的影響值得研究.目的:觀察納米羥基燐灰石-二氧化鋯生物陶瓷刀片材料切割傷口後對其愈閤的影響.設計:單一樣本觀察.單位:哈爾濱醫科大學附屬第二醫院骨二科,哈爾濱工業大學.材料:實驗于2006-03/05在哈爾濱醫科大學附屬第二醫院實驗動物研究室完成,選用54隻4月齡SD大鼠,雌雄不限,體質量120～180 g.實驗所使用的納米羥基燐灰石粉體為自行製備,Ca(NO3)2·4H2O(天津市博迪化工有限公司),(NH4)2HPO4(天津市石英鐘廠霸州化工分廠),氨水(綏化市化學試劑廠),無水乙醇(天津化學試劑廠).方法:將納米羥基燐灰石和二氧化鋯粉體經充分研磨後得到納米羥基燐灰石/二氧化鋯複閤粉體,以質量比按40%羥基燐灰石+60%二氧化鋯比例配製,採用熱壓燒結技術磨削製成具有一定刃形形狀的外形,進行傷口切割實驗,即用高壓滅菌後的生物陶瓷刃部縱行切開大鼠揹部皮膚與皮下組織,切口長為2 cm,再用1#絲線縫閤切口.分彆在術後第3,7,14天將動物斷頸處死,每次取18隻,切取切口處皮膚與皮下、肌肉組織進行大體觀察,同時進行切片、囌木精-伊紅染色,光學顯微鏡觀察傷口愈閤情況.主要觀察指標:大鼠揹部傷口大體及組織學觀察結果.結果:納入大鼠54隻,均進入結果分析,無脫失值.術後3 d,大鼠揹部傷口邊緣可見到錶皮撕裂的微細碎屑,切口未愈閤,切口邊緣有明顯的炎性細胞浸潤.術後7 d,切口邊緣仍可見到錶皮撕裂的微細碎屑,但已經開始齣現脩複反應,碎屑減少,切口變平整,開始愈閤,切口皮下組織內有明顯的炎性細胞浸潤.在高倍光學顯微鏡下觀察,創緣開始愈閤,可見到新生的膠原纖維充填于切口間,但膠原纖維較疏鬆,皮下組織內有大量的浸潤的白細胞,為組織脩複反應相.術後14 d,傷口創緣間被緻密的膠原纖維充填,皮下組織內的白細胞浸潤明顯減輕,新生的毛細血管內可見到正常的血細胞,切口間新形成的膠原纖維緻密堅彊,為正常創傷脩複與和組織.結論:納米羥基燐灰石-二氧化鋯生物陶瓷材料具有無毒、無過敏等特點,具有良好的生物活性.
배경:간기린회석시일충이상적골화아치적외과의용재료,단기강도저차구유일정적취성;간기린회석화이양화고(3%mol양화을은정적립방상이양화고)작위골체대재료,이자복합후제비생물도자재료대상구유합적영향치득연구.목적:관찰납미간기린회석-이양화고생물도자도편재료절할상구후대기유합적영향.설계:단일양본관찰.단위:합이빈의과대학부속제이의원골이과,합이빈공업대학.재료:실험우2006-03/05재합이빈의과대학부속제이의원실험동물연구실완성,선용54지4월령SD대서,자웅불한,체질량120～180 g.실험소사용적납미간기린회석분체위자행제비,Ca(NO3)2·4H2O(천진시박적화공유한공사),(NH4)2HPO4(천진시석영종엄패주화공분엄),안수(수화시화학시제엄),무수을순(천진화학시제엄).방법:장납미간기린회석화이양화고분체경충분연마후득도납미간기린회석/이양화고복합분체,이질량비안40%간기린회석+60%이양화고비례배제,채용열압소결기술마삭제성구유일정인형형상적외형,진행상구절할실험,즉용고압멸균후적생물도자인부종행절개대서배부피부여피하조직,절구장위2 cm,재용1#사선봉합절구.분별재술후제3,7,14천장동물단경처사,매차취18지,절취절구처피부여피하、기육조직진행대체관찰,동시진행절편、소목정-이홍염색,광학현미경관찰상구유합정황.주요관찰지표:대서배부상구대체급조직학관찰결과.결과:납입대서54지,균진입결과분석,무탈실치.술후3 d,대서배부상구변연가견도표피시렬적미세쇄설,절구미유합,절구변연유명현적염성세포침윤.술후7 d,절구변연잉가견도표피시렬적미세쇄설,단이경개시출현수복반응,쇄설감소,절구변평정,개시유합,절구피하조직내유명현적염성세포침윤.재고배광학현미경하관찰,창연개시유합,가견도신생적효원섬유충전우절구간,단효원섬유교소송,피하조직내유대량적침윤적백세포,위조직수복반응상.술후14 d,상구창연간피치밀적효원섬유충전,피하조직내적백세포침윤명현감경,신생적모세혈관내가견도정상적혈세포,절구간신형성적효원섬유치밀견강,위정상창상수복여화조직.결론:납미간기린회석-이양화고생물도자재료구유무독、무과민등특점,구유량호적생물활성.
BACKGROUND: Hydroxyapatite is a kind of ideal orthopedic material, but its Iow strength and brittleness need to improve. The research suggested that the improvement of the toughness and strength of bioceramics which composite hydroxyapatite (HAP) and zirconia (3% mol yttria-stabilized cubic zirconia) and fabricated ceramic scalpel possesses of the wound recover faster with smaller side. So it is significant that research on the incision concrescence of nano-sized hydroxyapatite-zirconia bioceramical scalpel.OBJECTIVE: To observe the effects of incision using nano-sized HAP-TZP bioceramics as scalpel.DESIGN: A single sample study.SETTING: Second Department of Orthopaedics, the Second Affiliated Hospital of Harbin Medical University and Harbin Institute of Technology.MATERIALS: The experiment was carried out in the Animal Laboratory of the Second Affiliated Hospital of Harbin Medical University (Provincial Laboratory) from March to May 2006. A total of 54 SD rats, of 4 months old, weighing 120-180 g, of both genders, were selected in this study. The experiment materials included nano-sized hydroxyapatite Quartz Clock Company), ammonia (Suihua Chemical Reagent Company), and anhydrous ethanol (Tianjin Chemical Reagent Company).METHODS: The nano-sized Hydroxyapatite/zirconia composite powder was synthesis by rubbing according to 40% hydroxyapatite + 60% zirconia powders (mass ratio) were mixed and milled. The knife-edge biomaterials, which fabricated by hot pressed sintering, was grinding and used in incision experiment after autoclaving. The back of the rats was shaved and removing a patch of skin under ether anesthesia made a length of 2 cm open excision wound and stitched up the tear by 1# string. The each 18 rats were sacrificed on 3, 7 and 14 days post-wounding and used for analyses respectively. A full-thickness specimen containing the wound was dissected out from each of the surgical sites. The hematoxylin-eosin (HE) staining was applied in the pathological observation.MAIN OUTCOME MEASURES: The tissue sections of back and the results of pathological observation. RESULTS: All 54 rats were involved in the results analysis without any loss. There was long gap with minimal cellular reaction in the scalpel wounds that has not healed on day 3. There was inflamed cells infiltration at the wounded margin edge. By week 1, the tiny detritus which some of them appear the repaired response of epidermis rend were still obviously found in the area of the scalpel wounds where the detritus was found to be significantly less, the wounds changed smooth and started to heal. The optical microscopy observed indicated that there was incomplete epithelialization and hyalinization of dermal collagen at the wounded area. There were obvious inflamed cells to infiltrate in the margin subcutaneous tissue of the wounds where the newborn loose collagenous fibers filled in scalpel wounds was the tissue repairing response phase. There was a great deal of the infiltrated white blood cells in the subcutaneous tissue. The tiny detritus of epidermis by the optical microscopy observed rend could not be found in the area of the scalpel wounds which were healed by week 2. The newborn capillary nets appeared in the newborn repaired collagen fibers tissue. The inflamed cells to be infiltrated were reduced. The compact and strong collagenous fibers filled in scalpel wounds were the normal wound repairing and tissue. The white blood cells infiltrated obvious reduction in the subcutaneous tissue. The normal blood cells in the newborn capillaries could be seen. CONCLUSION: The experiments prove that a HAP-ZTP bioceramic scalpel, which possesses excellent bioactive, is nontoxic, nonallergenic and noncarcinogenic for incisions.
