CAJ | 학술논문

细胞因子类%Th1细胞/代谢%Th2细胞/代谢%白细胞介素4/代谢%白细胞介素5/代谢%白细胞介素13/代谢%干扰素Ⅱ型/代谢%趋化因子类%RANTES/代谢%过敏反应/病理生理学細胞因子類%Th1細胞/代謝%Th2細胞/代謝%白細胞介素4/代謝%白細胞介素5/代謝%白細胞介素13/代謝%榦擾素Ⅱ型/代謝%趨化因子類%RANTES/代謝%過敏反應/病理生理學
세포인자류%Th1세포/대사%Th2세포/대사%백세포개소4/대사%백세포개소5/대사%백세포개소13/대사%간우소Ⅱ형/대사%추화인자류%RANTES/대사%과민반응/병리생이학
Cytokines%Th1 cells/metab%Th2 cells/metab%Interleukin-4/metab%Interleukin-5/metab%Interleukin-13/metab%Interferon type Ⅱ/metab%Chemotactic factor%RANTES/metab%Anaphylaxis/physiopathol

目的:检测过敏性疾病患者血清Th1细胞因子IFN-γ和Th2细胞因子IL-4、IL-5、IL-13以及趋化因子Eotaxin、RANTES、LTB4的水平,探讨其临床意义.方法:过敏性疾病患者64例,正常对照21例.采用双抗体夹心酶联免疫吸附实验(ELISA)检测血清IL-4、IL-5、IL-13、IFN-γ、LTB4、RANTES和 Eotaxin水平.结果:①过敏性疾病患者血清IL-4、IL-5、IL-13和 Eotaxin、LTB4水平较对照组明显增高,差异有统计学意义(P<0.05).②IFN-γ和RANTES在过敏性疾病患者血清中的水平低于对照组,但无统计学差异(P>0.05).③IL-4、IL-5、IL-13和LTB4彼此之间呈显著正相关(P<0.01).④Eotaxin、RANTES和IFN-γ彼此之间呈显著正相关(P<0.05).⑤LTB4和Eotaxin之间呈显著正相关(P<0.01).结论:过敏性疾病的发生存在多种细胞因子和趋化因子复杂的相互作用.
목적:검측과민성질병환자혈청Th1세포인자IFN-γ화Th2세포인자IL-4、IL-5、IL-13이급추화인자Eotaxin、RANTES、LTB4적수평,탐토기림상의의.방법:과민성질병환자64례,정상대조21례.채용쌍항체협심매련면역흡부실험(ELISA)검측혈청IL-4、IL-5、IL-13、IFN-γ、LTB4、RANTES화 Eotaxin수평.결과:①과민성질병환자혈청IL-4、IL-5、IL-13화 Eotaxin、LTB4수평교대조조명현증고,차이유통계학의의(P<0.05).②IFN-γ화RANTES재과민성질병환자혈청중적수평저우대조조,단무통계학차이(P>0.05).③IL-4、IL-5、IL-13화LTB4피차지간정현저정상관(P<0.01).④Eotaxin、RANTES화IFN-γ피차지간정현저정상관(P<0.05).⑤LTB4화Eotaxin지간정현저정상관(P<0.01).결론:과민성질병적발생존재다충세포인자화추화인자복잡적상호작용.
Objective: To determine Th1/Th2 cytokines and chemokines in patients with allergic diseases and its clinic significance. Methods: Serum levels of IFN-γ,IL-4,IL-5,IL-13,Eotaxin,RANTES and LTB4 were determined from peripheral blood of 64 allergic patients and 21 healthy controls with ELISA. Results: IL-4,IL-5,IL-13 and Eotaxin,LTB4 were increased significantly in serum of allergic patients compared with those of controls (P<0.05).There were no significant differences in serum levels of IFN-γ and RANTES between patients and controls (P>0.05).Serum levels of IL-4,IL-5,IL-13 and LTB4 were correlated with each other(P<0.01).Eotaxin,RANTES and IFN-γ levels were also significantly correlated with each other(P<0.05).LTB4 was correlated with Eotaxin as well(P<0.01). Conclusion: A wide range of cytokines and chemokines is involved in allergic diseases,which may play their roles in a complex interactive manner.