CAJ | 학술논문

反义抑制ghrelin对VEGF及其受体Flt-1 mRNA表达的影响
반의억제ghrelin대VEGF급기수체Flt-1 mRNA표체적영향
Effects of Ghrelin Antisense Inhibition on VEGF and Its Receptor Flt-1 mRNA Expression

万方数据

农业科学与技术（英文版）農業科學與技術（英文版） 농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2009年 5期 45-48 ,共4页

姜丽萍%祝啸先%游存厚%乌日古木拉%王芳%张文娟%刘德斌%杜晨光%李海军%包福祥%赵鹏伟%鲍庆江%曹贵方

강려평%축소선%유존후%오일고목랍%왕방%장문연%류덕빈%두신광%리해군%포복상%조붕위%포경강%조귀방

生长素%血管内皮生长因子%受体%寡核苷酸类%反义生長素%血管內皮生長因子%受體%寡覈苷痠類%反義
생장소%혈관내피생장인자%수체%과핵감산류%반의
Ghrelin%Vascular endothelial growth factor%Receptors%Oligonucleotides%Antisense

[目的] 探讨ghrelin对蒙古绵羊脐静脉内皮细胞的血管内皮生长因子VEGF及其受体Flt-1 mRNA表达的影响.[方法] 试验分为4组: I组,空白对照组;II组,脂质体组(不加寡核苷酸);III组(SCON),20 μmol/L正义寡核苷酸组;IV组(ASCON),20 μmol/L反义寡核苷酸组.上述各组均在培养24、36、48 h后,采用实时荧光定量方法检测VEGF及其受体Flt-1 mRNA表达情况的变化.[结果] VEGF和Flt-1熔解曲线表明扩增效果好,培养24 h, VEGF mRNA I组相对表达量为0.076 9,II组为0.076 8,III组为0.076 9,3组之间无显著性差异(P＞0.05),IV组VEGF mRNA 相对表达量下降为0.024 2,且与I、II、III组有显著性差异(P＜0.05).培养36 h,前3组相对表达量分别为0.059,0.051,0.051 4,3组之间无显著性差异(P＞0.05),第IV组为0.024 2,与前3组比较有显著性差异(P＜0.05).培养48 h,前3组相对表达量分别为0.061,0.055 2,0.052 1,3组之间无显著性差异(P＜0.05),第IV组为0.011 5,与前3组比较有显著性差异(P＜0.05).培养24 h,Flt-1 mRNA I组相对表达量为0.078 6;II组为0.078 2,III组为0.078 1, 3组之间无显著性差异(P＞0.05),IV组Flt-1 mRNA 相对表达量下降为0.025 6,且与I、II、III组有显著性差异(P＜0.05).培养36 h,前3组相对表达量分别为0.078 1,0.078 4,0.078 2,3组之间无显著性差异(P＞0.05),第IV组为0.022 8,与前3组比较有显著性差异(P＜0.05).培养48 h,前3组相对表达量分别为0.069 1,0.078 0,0.069 3,3组之间无显著性差异(P＞0.05),第IV组为0.021 5,与前3组比较有显著性差异(P＜0.05).可见,VEGF受体Flt-1mRNA的表达与VEGF相似.二者均在 I组、II组表达量无差异,具有较高的表达量,随着时间的延长没有明显变化;在III组表达轻微下降,但与I组和II组无显著性差异(P＞0.05);IV组的表达量显著下降(P＜0.05),并且随着时间的延长至逐渐下降.[结论] 反义抑制ghrelin对VEGF及其受体Flt-1的mRNA表达有下调作用.
[목적] 탐토ghrelin대몽고면양제정맥내피세포적혈관내피생장인자VEGF급기수체Flt-1 mRNA표체적영향.[방법] 시험분위4조: I조,공백대조조;II조,지질체조(불가과핵감산);III조(SCON),20 μmol/L정의과핵감산조;IV조(ASCON),20 μmol/L반의과핵감산조.상술각조균재배양24、36、48 h후,채용실시형광정량방법검측VEGF급기수체Flt-1 mRNA표체정황적변화.[결과] VEGF화Flt-1용해곡선표명확증효과호,배양24 h, VEGF mRNA I조상대표체량위0.076 9,II조위0.076 8,III조위0.076 9,3조지간무현저성차이(P＞0.05),IV조VEGF mRNA 상대표체량하강위0.024 2,차여I、II、III조유현저성차이(P＜0.05).배양36 h,전3조상대표체량분별위0.059,0.051,0.051 4,3조지간무현저성차이(P＞0.05),제IV조위0.024 2,여전3조비교유현저성차이(P＜0.05).배양48 h,전3조상대표체량분별위0.061,0.055 2,0.052 1,3조지간무현저성차이(P＜0.05),제IV조위0.011 5,여전3조비교유현저성차이(P＜0.05).배양24 h,Flt-1 mRNA I조상대표체량위0.078 6;II조위0.078 2,III조위0.078 1, 3조지간무현저성차이(P＞0.05),IV조Flt-1 mRNA 상대표체량하강위0.025 6,차여I、II、III조유현저성차이(P＜0.05).배양36 h,전3조상대표체량분별위0.078 1,0.078 4,0.078 2,3조지간무현저성차이(P＞0.05),제IV조위0.022 8,여전3조비교유현저성차이(P＜0.05).배양48 h,전3조상대표체량분별위0.069 1,0.078 0,0.069 3,3조지간무현저성차이(P＞0.05),제IV조위0.021 5,여전3조비교유현저성차이(P＜0.05).가견,VEGF수체Flt-1mRNA적표체여VEGF상사.이자균재 I조、II조표체량무차이,구유교고적표체량,수착시간적연장몰유명현변화;재III조표체경미하강,단여I조화II조무현저성차이(P＞0.05);IV조적표체량현저하강(P＜0.05),병차수착시간적연장지축점하강.[결론] 반의억제ghrelin대VEGF급기수체Flt-1적mRNA표체유하조작용.
[Objective] This study was to investigate the effect of VEGF and its receptor Flt-1 mRNA expression in Mongolia sheep umbilical vein endothelial cells by ghrelin antisense inhibition. [Method] Experiments were divided into 4 groups: group I (blank control group); group II (liposome group); group III (SCON group: 20 μmol/L sense oligonucleotide); group IV (ASCON: 20 μmol/L antisense oligonucleotide). VEGF and its receptor Flt-1 mRNA expression changes were detected by using real-time fluorescence quantitative detection after 24, 36 and 48 h. [Result] The expression of VEGF mRNA in group I, group II were insignificantly different at higher expression levels, and did not change significantly with the time; the expression of VEGF mRNA in group III assumed a slight decrease, but there were no significant differences between group I and group II (P>0.05), the expression of VEGF mRNA in group IV(antisense oligonucleotide group ) decreased significantly (P< 0.05); the expression of VEGF receptor FLT-1 mRNA was similar to that of VEGF. [Conclusion] Antisense inhibition ghrelin has a downward effect to the expression of VEGF and its receptor Flt-1 the mRNA.