CAJ | 학술논문

酶的分子定向进化是20世纪90年代初兴起的一种蛋白质工程的新策略,是一种在生物体外模拟自然进化过程的、具有一定目的性的快速改造蛋A质的方法.该方法引起了生物催化技术领域的又一次革命.目前分子定向进化技术已被广泛应用于工业、农业及制药业等的相关领域.本文详细综述了酶的分子定向进化的概念、过程、基本策略及其核心技术,并着重介绍了酶的分子定向进化技术在提高酶的活力、稳定性、底物特异性和对映体选择性等几方面的应用及取得的相关成果.
매적분자정향진화시20세기90년대초흥기적일충단백질공정적신책략,시일충재생물체외모의자연진화과정적、구유일정목적성적쾌속개조단A질적방법.해방법인기료생물최화기술영역적우일차혁명.목전분자정향진화기술이피엄범응용우공업、농업급제약업등적상관영역.본문상세종술료매적분자정향진화적개념、과정、기본책략급기핵심기술,병착중개소료매적분자정향진화기술재제고매적활력、은정성、저물특이성화대영체선택성등궤방면적응용급취득적상관성과.
Directed molecular evolution of enzyme,originated in the beginning of the nineties of the last century,was a new strategy of protein engineering,which could rapidly modify proteins for specific purpose through imitating extemally natural evolutions,as well as it has arisen a revolution in biocatalyst field.Nowadays,this technology has been widely applied in various fields including chemical engineering,agriculture and pharmaceutical industry.This paper elaborates on the concept of directed molecular evolution,procedure,basic strategies,and its core technology,then presents emphatically its application and achievements in improving enzyme activities,stability,substrate specificity,and antipode selectivity and so on.