CAJ | 학술논문

旨在研究β-catenin在不同毛色羊驼皮肤中的表达和定位,探索其与毛色的关系.以成年白色和棕色羊驼为研究对象,采用实时荧光定量PCR技术、Western blot和免疫组织化学技术,对β-catenin在白色和棕色羊驼皮肤中mRNA、蛋白表达水平和定位进行研究.实时荧光定量PCR结果显示,β-catenin在棕色羊驼皮肤组织中的相对基因表达量是白色羊驼皮肤组织的1. 662倍;Western blot结果显示,羊驼皮肤组织粗蛋白提取物中存在分子量约85 ku与兔抗β-catenin多克隆抗体发生免疫阳性反应的蛋白条带,棕色羊驼平均蛋白表达量显著高于白色羊驼;免疫组织化学结果显示,β-catenin在羊驼皮肤的表皮、毛乳头、毛根鞘和皮脂腺中表达,根据光密度值得出,除皮脂腺之外,在表皮、毛乳头和毛根鞘的表达差异极显著(P<0.01).结果提示β-catenin在白色和棕色羊驼皮肤组织中定位以及表达的差异,表明β-catenin参与毛色形成.
지재연구β-catenin재불동모색양타피부중적표체화정위,탐색기여모색적관계.이성년백색화종색양타위연구대상,채용실시형광정량PCR기술、Western blot화면역조직화학기술,대β-catenin재백색화종색양타피부중mRNA、단백표체수평화정위진행연구.실시형광정량PCR결과현시,β-catenin재종색양타피부조직중적상대기인표체량시백색양타피부조직적1. 662배;Western blot결과현시,양타피부조직조단백제취물중존재분자량약85 ku여토항β-catenin다극륭항체발생면역양성반응적단백조대,종색양타평균단백표체량현저고우백색양타;면역조직화학결과현시,β-catenin재양타피부적표피、모유두、모근초화피지선중표체,근거광밀도치득출,제피지선지외,재표피、모유두화모근초적표체차이겁현저(P<0.01).결과제시β-catenin재백색화종색양타피부조직중정위이급표체적차이,표명β-catenin삼여모색형성.
The objective of this study was to study the expression and localization of β-catenin in alpaca skin of different colors,and to explore the function of β-catenin in the process of coat color formation.The mRNA and protein expression level of β-catenin in alpaca skin of different colors were examined by Real-time quantitative PCR,Western blot and immunohistochemistry.The gene expressive quantity of β-catenin in the brown alpaca's skin tissue was 1.662 times than ones of white alpaca.The distribution of β-catenin in alpaca skin were demonstrated,the expression was notable difference between the white and brown alpaca's based on the average optical density.Our findings showed that β-catenin was involved in the process of coat color formation.