CAJ | 학술논문

为了研究环境污染物四氯联苯对移植的原始生殖细胞增殖性迁移的影响,将发育至14期的供体鸡胚原始生殖细胞用PKH26荧光染料标记,然后移植到经过四氯联苯处理的受体鸡胚生殖新月区域,继续孵化,检测其迁移和增殖情况.结果表明,和对照组相比,四氯联苯可明显降低移植原始生殖细胞在血液和性腺中的数量;四氯联苯对外源性原始生殖细胞迁移有明显影响,但雌二醇对外源性原始生殖细胞的增殖性迁移无明显影响,揭示四氯联苯可能影响的是受体性腺的诱引物质,且这种影响是非雌激素样的作用.
위료연구배경오염물사록련분대이식적원시생식세포증식성천이적영향,장발육지14기적공체계배원시생식세포용PKH26형광염료표기,연후이식도경과사록련분처리적수체계배생식신월구역,계속부화,검측기천이화증식정황.결과표명,화대조조상비,사록련분가명현강저이식원시생식세포재혈액화성선중적수량;사록련분대외원성원시생식세포천이유명현영향,단자이순대외원성원시생식세포적증식성천이무명현영향,게시사록련분가능영향적시수체성선적유인물질,차저충영향시비자격소양적작용.
To stuey the effects of tetmchlorobiphenyl on the migration of the primordial germ cells(PGCs)multiplication with utilizing poultry embryonic primordial germ cell migration features,the donor PGCs isolated from the chick embryos at stage 14 were labelled with PKH26.The labelled PGCs were transplanted into the germinal crescents of recipient embryos treated with tetrachlorobiphenyl,which were then incubated.The labelled donor PGCs were detected at stages 13～15 and stages 27～29.The number of the exogenous PGCs decreased significantly at stages 13～15 and stages 27～29 compared with controls(P<0.01).Tetrachlorobiphenyl had significantly effect on the migration of exogenous PGCs,but estradiol had no effect.The result suggests that tetrachlorobiphenyl may have the impact of toxicity,rather than estrogen-like role.