CAJ | 학술논문

目的观察兔肝组织经低剂量高强度聚焦超声(HIFU)辐照后的病理转归,探讨低剂量HIFU辐照改变兔肝组织声环境的作用及机制. 方法对10只新西兰大白兔分别于低剂量HIFU辐照其肝脏后即刻(第1天)、2、3、5及7天取材进行光镜和电镜观察. 结果光镜下组织学变化:辐照后即刻,可见肝细胞水肿;第2、3天可见淤血,水肿加重;随后肝细胞逐渐恢复正常.电镜下超微结构变化:辐照后即刻,可见肝血窦内皮细胞脱落,肝细胞胞浆内细胞器肿胀;第2、3天可见肝血窦内红细胞聚集,肝细胞胞浆内出现空泡;随后细胞器肿胀逐渐减轻,直至恢复正常. 结论低剂量HIFU辐照可使兔肝组织发生相应的变化,从而改变组织声环境.
목적 관찰토간조직경저제량고강도취초초성(HIFU)복조후적병리전귀,탐토저제량HIFU복조개변토간조직성배경적작용급궤제. 방법 대10지신서란대백토분별우저제량HIFU복조기간장후즉각(제1천)、2、3、5급7천취재진행광경화전경관찰. 결과 광경하조직학변화:복조후즉각,가견간세포수종;제2、3천가견어혈,수종가중;수후간세포축점회복정상.전경하초미결구변화:복조후즉각,가견간혈두내피세포탈락,간세포포장내세포기종창;제2、3천가견간혈두내홍세포취집,간세포포장내출현공포;수후세포기종창축점감경,직지회복정상. 결론 저제량HIFU복조가사토간조직발생상응적변화,종이개변조직성배경.
Objective To observe the pathological regression of rabbit liver after the low-dose irradiation with high intensity focused ultrasound (HIFU), and to explore the role and mechanism of the irradiation in changing acoustic environment of rabbit liver. Methods Pathological study was performed in 10 New Zealand white rabbits, the livers were observed under light and electron microscope. The observation was done immediately after low-dose HIFU irradiation and in 2, 3, 5, 7 days after the low-dose irradiation with HIFU. Results Light microscope changes: Edema existed in hepatocytes after irradiation instantly, congestion and aggravated edema were found at 2-3 days, then the injured cells recovered gradually. Electron microscope changes: Detached endothelial cells of hepatic sinusoid and swelling organelles were observed after irradiation, while 2 or 3 days later, erythrocyte aggregation was found in hepatic sinusoid and cavitation was found in cytoplasm. Thereafter, organelles swelling reduced till resumed to normal. Conclusion Low-dose HIFU irradiation can make corresponding changes to the acoustic environment of rabbit liver.