Cloning the full-length cDNA of a gene responsible for vascular smooth muscle cell proliferation in atherogenesis and study of function
  • 万方数据
    中华医学杂志(英文版) 중화의학잡지(영문판)
    CHINESE MEDICAL JOURNAL
    2003年 2期 166-170 ,共5页

    赵高峰%高鹏%张华%娄黎明%乔绘红%佘铭鹏

    조고봉%고붕%장화%루려명%교회홍%사명붕



    subtractive hybridization%associated gene%cDNA%whole length%functional study


    Objective To clone the full-length cDNA of a gene responsible for vascular smooth muscle cell (v-SMC) proliferation in atherogenesis, and study its function.Methods Oxidized low density lipoprotein (ox-LDL) at optimal concentration was used as the stimulant to induce v-SMC prol iferation in culture medium. AcDNA subtractive library of v-SMC proliferation specific toox-LDL stimulation was established using subtractive hybridization technique. Methods, including blotting, Northern hybridization and gene sequen cing, were used to clone new gene fragments. By using full-length cDNA screeni ng and protein expression techniques, one full-length cDNA was cloned and its f unction was studied. Results One full-length cDNA was cloned. The new gene (Genbank AF 174647) expressed a 44 kDa protein, which might be associated with the activity of ox-LDL. Conclusion The new gene cloned may be associated with SMC proliferation in atherogenesis.
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