CAJ | 학술논문

目的探讨黄芪联合福辛普利对白蛋白诱导肾小管上皮细胞内NF-κB活性及骨桥蛋白表达的影响.方法培养的肾小管上皮细胞为空白对照;对照组加去脂的小牛血清白蛋白(BSA)20 ms/ml共同培养;干预组先加福辛普利5 μmol/L,1/2 h后再加入BSA 20 mg/ml,黄芪组在干预组基础上加黄芪40 μl/ml,1/2 h后再加入BSA20 mg/ml,凝胶电泳迁移率法(EMSA)法及RTPCR检测12 h、24 h各组NF-κB活性及骨桥蛋白表达的变化.结果黄芪组在12 h,24 h均可明显抑制白蛋白诱导的肾小管上皮细胞内NF-κB活性及骨桥蛋白的表达,与空白、对照组及干预组比较差异有统计学意义(P<0.05).结论黄芪联合福辛普利可明显抑制白蛋白诱导的肾小管上皮细胞内NF-κB活性及骨桥蛋白的表达.
목적 탐토황기연합복신보리대백단백유도신소관상피세포내NF-κB활성급골교단백표체적영향.방법 배양적신소관상피세포위공백대조;대조조가거지적소우혈청백단백(BSA)20 ms/ml공동배양;간예조선가복신보리5 μmol/L,1/2 h후재가입BSA 20 mg/ml,황기조재간예조기출상가황기40 μl/ml,1/2 h후재가입BSA20 mg/ml,응효전영천이솔법(EMSA)법급RTPCR검측12 h、24 h각조NF-κB활성급골교단백표체적변화.결과 황기조재12 h,24 h균가명현억제백단백유도적신소관상피세포내NF-κB활성급골교단백적표체,여공백、대조조급간예조비교차이유통계학의의(P<0.05).결론 황기연합복신보리가명현억제백단백유도적신소관상피세포내NF-κB활성급골교단백적표체.
Objective To explore whether or not the function of atragalus membranaceus combined fosinopril on decreasing NF- κB activity and OPN expression in overload albumin stimulating proximal tubuler cells. Methods Cultured cells without stimulation were used as placebo. Cultured cells were incubated with bovine serum albumin fat acid free (BSA) 20mg/ml as the control. Cultured cells were incubated with fosinopril for different hours as the treatment. Cultured cells were incubated with atragalus membranaceus combined fosinopril for different hours as the atragalus membranaceus group. ELISA and RT-PCR were used to observe NF -κB activity and OPN expression after cultured for 12h, 24h. Result Atragalus membranaceus combined fosinopril rapidly decreased NFκB activity and OPN expression. Compared with the other groups, the difference was significant. Conclusion Astragalus membranaceus combined fosinopril can obviously decrease NFκB activity and OPN expression in overload albumine stimulating proximal tubular cells.