CAJ | 학술논문

目的观察骨形态发生蛋白-2(BMP-2)和转化生长因子-β1 (TGF-β1)双基因真核共表达载体对兔骨髓基质细胞(MSCs)向软骨细胞分化mRNA表达的影响。方法 pIRES-BMP-2-TGF-β1、pIREES-BMP-2 and pIRES-TGF-β1质粒通过质脂体介导转染MSCs,采用荧光定量逆转录-聚合酶链反应(RT-PCR)方法检测MSCs内Ⅱ型胶原和蛋白多糖mRNA的表达。结果转染后2、4d双基因组MSCs内的Ⅱ型胶原和蛋白多糖mRNA表达量60.4±10.1、606.0±88.5和42.6±6.0、411.2±73.6均明显高于转染后单一BMP-2(28.7±4.0、236.7±48.5和26.9±4.3、208.2±36.7)及TGF-β1(30.9±4.54、205.5±38.7和28.4±3.7、184.9±30.9)组(P＜0.05)。结论 pIRES-BMP-2-TGF-β1双基因真核共表达载体诱导MSCs向软骨细胞分化的作用强于单一基因BMP-2及TGF-β1。
목적 관찰골형태발생단백-2(BMP-2)화전화생장인자-β1 (TGF-β1)쌍기인진핵공표체재체대토골수기질세포(MSCs)향연골세포분화mRNA표체적영향。방법 pIRES-BMP-2-TGF-β1、pIREES-BMP-2 and pIRES-TGF-β1질립통과질지체개도전염MSCs,채용형광정량역전록-취합매련반응(RT-PCR)방법검측MSCs내Ⅱ형효원화단백다당mRNA적표체。결과 전염후2、4d쌍기인조MSCs내적Ⅱ형효원화단백다당mRNA표체량60.4±10.1、606.0±88.5화42.6±6.0、411.2±73.6균명현고우전염후단일BMP-2(28.7±4.0、236.7±48.5화26.9±4.3、208.2±36.7)급TGF-β1(30.9±4.54、205.5±38.7화28.4±3.7、184.9±30.9)조(P＜0.05)。결론 pIRES-BMP-2-TGF-β1쌍기인진핵공표체재체유도MSCs향연골세포분화적작용강우단일기인BMP-2급TGF-β1。
Objective To observe the effect of a bicistronic eukaryotic expression plasmid pIRESbone morphogenetic protein (BMP)-2-transforming growth factor (TGF)-β1 on the mRNA expression during the chondrocytic differention of bone marrow stromal cells (MSCs). MethodspIRES-BMP-2-TGF-β1,pIRES-BMP-2 and pIRES-TGF-β1 plasmids were transfected to MSCs by lipids. The mRNA expression of type Ⅱ collagen and proteoglycan was detected by using the quantitative polymerase chain reaction (PCR)technique. Results At the 2nd, and 4th day after the plasmids of each group were transfected to MSCs,the gene expression in MSCs of each group examined by quantitative PCR showed that type Ⅱ collagen and proteoglycan gene expression levels in MSCs of pIRES-BMP-2-TGF-β1 group were significantly higher than those of pIRES-BMP-2 and pIRES-TGF-β1 groups ( P ＜ 0. 05 ). Conclusion The effect that pIRES-BMP2-TGF-β1 induced MSCs differentiation towards chondrocytes was greater than that of pIRES-BMP-2 or pIRES-TGF-β1 alone.