CAJ | 학술논문

TPR(tetratricopeptide repeat)是在很多蛋白中均被发现到的一个含有34个氨基酸的蛋白重复序列,其基本功能是参与蛋白间的相互作用.天蓝色链霉菌A3(2)中有70个蛋白含有类TPR结构域,NsdA是其中的一个,研究发现该蛋白对天蓝色链霉菌的产孢和产素都有负调控作用.本研究中发现基因SCO7252和SCO1593编码含TPR结构的蛋白,中断SCO7252基因后菌株放线紫红素和钙依赖抗生素产量均提高,但形态分化没有明显变化,基因SCO1593中断后菌株在产孢产素及形态等各方面均未受到影响.基因SCO7252被命名为nsdB,RT-PCR分析表明,该基因在生长30h时开始表达.通过生物信息分析表明,天蓝色链霉菌的70个含类TPR结构的蛋白中有32个仅含该结构域,有25个另外含有DNA结合区域,这些暗示着它们可能直接控制基因的表达.
TPR(tetratricopeptide repeat)시재흔다단백중균피발현도적일개함유34개안기산적단백중복서렬,기기본공능시삼여단백간적상호작용.천람색련매균A3(2)중유70개단백함유류TPR결구역,NsdA시기중적일개,연구발현해단백대천람색련매균적산포화산소도유부조공작용.본연구중발현기인SCO7252화SCO1593편마함TPR결구적단백,중단SCO7252기인후균주방선자홍소화개의뢰항생소산량균제고,단형태분화몰유명현변화,기인SCO1593중단후균주재산포산소급형태등각방면균미수도영향.기인SCO7252피명명위nsdB,RT-PCR분석표명,해기인재생장30h시개시표체.통과생물신식분석표명,천람색련매균적70개함류TPR결구적단백중유32개부함해결구역,유25개령외함유DNA결합구역,저사암시착타문가능직접공제기인적표체.
Tetratricopeptide repeat (TPR) domains usually mediate protein-protein interactions. NsdA, one of the 70 proteins containing TPR-like domains in Streptomyces coelicolor A3(2), was previously found to negatively control sporulation and antibiotic production. Here we show that elimination of SCO7252, which encodes another of these proteins, also caused overproduction of two antibiotics, actinorhodin and CDA,but did not affect morphological differentiation. Disruption of SCO1593, encoding another of the family, had no obvious phenotypic effects. In surface-grown cultures, expression of SCO7252, which was named nsdB, was initiated at about 30 h, like that of nsdA. Analysis in silico of the 70 predicted TPR-like-containing proteins of S. coelicolor showed that 32 of them contained only TPR-like domains, and 25 of the remainder contained additional DNA-binding domains, implying that they might control gene expression directly.