CAJ | 학술논문

在常温(28℃)下用稀硫酸、氢氧化钠、氨水、过氧化氢溶液处理稻草原料,以实际糖化率为衡量指标对预处理效果进行比较.结果表明2%氢氧化钠溶液预处理效果最好.稻草原料通过温和的碱预处理后,绝大部分木质素被去除,但仍然有超过一半的半纤维素残留.以康宁木霉(T. koningii)QF-02生产的复合酶比两种商品纤维素酶制剂能更有效酶解糖化碱预处理稻草.自制复合酶的最适温度和最适pH分别为50℃和4.8;考虑酶解效率和操作费用,酶解时间48 h、酶载量10 FPU/g稻草、底物浓度8%(w/v)是合理的选择.
재상온(28℃)하용희류산、경양화납、안수、과양화경용액처리도초원료,이실제당화솔위형량지표대예처리효과진행비교.결과표명2%경양화납용액예처리효과최호.도초원료통과온화적감예처리후,절대부분목질소피거제,단잉연유초과일반적반섬유소잔류.이강저목매(T. koningii)QF-02생산적복합매비량충상품섬유소매제제능경유효매해당화감예처리도초.자제복합매적최괄온도화최괄pH분별위50℃화4.8;고필매해효솔화조작비용,매해시간48 h、매재량10 FPU/g도초、저물농도8%(w/v)시합리적선택.
Dilute solutions of H2SO4,NaOH,ammonia and H2O2 were evaluated as pretreatment reagents for improving the enzymatic saccharification of rice straw at room temperature(28℃).The highest saccharification rate was achieved in the case of rice straw pretreated with 2%NaOH.Mild alkali pretreatment resulted in a majority removal of lignin,but higher than half of hemicellulose was remained in the material.The compound enzyme produced by T. koningii QF-02 Was more suitable for the hydrolysis of pretreated rice straw than the mixture of two commercial cellulases.The optimum temperature and pH for the hydrolysis of pretreated rice straw of the compound enzyme was 50℃ and 4.8respectively. For the hydrolysis of alkali pretreated rice straw by the compound enzyme,the optimal hydrolysis time,enzyme loading and substrate concentration were determined as 48 h,10 FPU/g biomass and 8%(w/v),respectively.