CAJ | 학술논문

除草剂在田间的重复及不合理使用,导致了杂草抗药性的发生和发展.其中AHAS抑制剂由于靶标单一,抗性发展十分迅速.截至2009年,已有103种杂草对AHAS抑制剂产生了抗药性,占19类化学除草剂总抗药性杂草生物型的近1/3.从AHAS基因突变位点及种类与杂草抗药性水平的关系、AHAS基因突变与AHAS酶活性的关系、AHAS基因拷贝数与杂草抗药性的关系以及AHAS酶与除草剂结合前后的三维结构等方面,综述了杂草对AHAS抑制剂产生抗药性的机理,旨在为AHAS抑制剂抗性研究提供参考.并对自然种群目标基因的等位基因检测技术(ECOTILLING)和衍生型酶切扩增多态性序列(dCAPS)两种通过检测等位基因多态性的手段快速诊断抗药性杂草的新技术进行了介绍,讨论了延缓杂草抗药性发生和发展的策略.
제초제재전간적중복급불합리사용,도치료잡초항약성적발생화발전.기중AHAS억제제유우파표단일,항성발전십분신속.절지2009년,이유103충잡초대AHAS억제제산생료항약성,점19류화학제초제총항약성잡초생물형적근1/3.종AHAS기인돌변위점급충류여잡초항약성수평적관계、AHAS기인돌변여AHAS매활성적관계、AHAS기인고패수여잡초항약성적관계이급AHAS매여제초제결합전후적삼유결구등방면,종술료잡초대AHAS억제제산생항약성적궤리,지재위AHAS억제제항성연구제공삼고.병대자연충군목표기인적등위기인검측기술(ECOTILLING)화연생형매절확증다태성서렬(dCAPS)량충통과검측등위기인다태성적수단쾌속진단항약성잡초적신기술진행료개소,토론료연완잡초항약성발생화발전적책략.
Repeated and unreasonable field application of herbicides have resulted in the evolution of resistant weed species.AHAS is the target site of AHAS-inhibiting herbicides.By 2009,103 weed biotypes have showed resistance to AHAS-inhibiting herbicides,accounting for nearly 1/3 of total weed biotypes resistant to 19 chemical groups.The relationship between AHAS gene mutation sites/types and the level of herbicide resistance,AHAS gene mutations and AHAS activity,AHAS gene number and herbicide resistance,and the 3D structure of AHAS before and after binding with AHAS-inhibiting herbicides were reviewed.ECOTILLING and dCAPS,two new techniques for rapidly diagnosing herbicide resistance by detecting SNP were brought forward,and potential management strategy for preventing and delaying the herbicide resistance evolution in weeds was also discussed.