CAJ | 학술논문

利用42个PAPD引物对75份陕西大豆种质进行遗传多样性分析,共扩增出310个条带,平均每个引物扩增7.3个条带,多态性比率为96%;田间试验考察了13个农艺性状.陕西大豆的遗传多样性在秦岭南、北两个地区有所不同,秦岭北品种遗传多样性指数较高的性状数目和性状遗传多样性指数都大于秦岭南品种,RAPD分子标记遗传多样性指数也是秦岭北品种大于秦岭南品种,但秦岭南品种RAPD分子标记的遗传多样性指数较高的个数大于秦岭北品种.聚类分析将参试大豆材料分为三大类,基本上反映了材料的地理来源.主成分分析结果显示,前两个主成分反映了10.95%的遗传变异,基于前两个主成分值的二维散点图可以将两个地区的材料基本区分开来.AMOVA分析显示,陕西大豆品种个体问的遗传变异占总变异的92.06%.地区间的遗传变异占总变异的7.94%,二者都达到了极显著水平.研究结果表明,陕西大豆资源存在丰富的遗传多样性,秦岭北品种遗传多样性较高,但秦岭南品种有着广泛的微小变异.
이용42개PAPD인물대75빈협서대두충질진행유전다양성분석,공확증출310개조대,평균매개인물확증7.3개조대,다태성비솔위96%;전간시험고찰료13개농예성상.협서대두적유전다양성재진령남、북량개지구유소불동,진령북품충유전다양성지수교고적성상수목화성상유전다양성지수도대우진령남품충,RAPD분자표기유전다양성지수야시진령북품충대우진령남품충,단진령남품충RAPD분자표기적유전다양성지수교고적개수대우진령북품충.취류분석장삼시대두재료분위삼대류,기본상반영료재료적지리래원.주성분분석결과현시,전량개주성분반영료10.95%적유전변이,기우전량개주성분치적이유산점도가이장량개지구적재료기본구분개래.AMOVA분석현시,협서대두품충개체문적유전변이점총변이적92.06%.지구간적유전변이점총변이적7.94%,이자도체도료겁현저수평.연구결과표명,협서대두자원존재봉부적유전다양성,진령북품충유전다양성교고,단진령남품충유착엄범적미소변이.
75 Shaanxi soybean landraces were selected and a randomized blocked design was executed,then get date of 13 agronomic traits.Extracted DNA from five seeds of each landrace,amplified DNA with 42 RAPD primers,and analyzed genetic diversity index of agronomic traits and RAPD bands.42 RAPD primers were selected from 648 primers,and then 310 fragments were produced,an average of 7.3 fragments for each primer,the polymorphic percentage was 96%.The genetic diversity of Shaanxi soybean differs in different regions.Either the number of the highest diversity trait or the mean diversity value was higher in region North Qinling.The mean value of genetic diversitv of RAPD bands were higher in region North Qinling while lower in region South Qinling,but the number of higher genetic diversity index of RAPD bands in region South Qinling was higher than that in north.Three groups were got from cluster analysis,landraces from same regions were tend to one cluster,clusters generally reflected the geographical origin of landraces.The first two principal components account for 10.95%of the total variation,but it separated landraces of the two regions.Landraces of different regions showed significant difference by AMOVA analvsis,variation between and within populations account for 7.94% and 92.06% of the total variation respectively,which were significant(P<0.0001).The genetic diversity of soybean germplasm was higher in region North Qinling,although there were many tiny variations existed in population of region South Qinling.Populations of landraces divided by Mount Qinling showed significant difference.