CAJ | 학술논문

目的利用64排螺旋CT全肝灌注模式成像测定中华田园犬肝纤维化模型的相关灌注参数并与病理对照来反映肝纤维化程度,分析其与VEGF表达水平的相关性.方法采用腹腔注射50％四氯化碳油溶液辅以高脂饮食,建立犬肝纤维化模型,定期行64排螺旋CT全肝灌注成像及肝穿活检.根据病理分期分析各组CT灌注参数,并与VEGF表达水平作对照研究.结果成功获得各期肝纤维化模型.对照组HAP(28.25 ±2.19) ml/(min·100 g),PVP( 53.53±10.71) ml/( min ·100 g),TLP(81.78±18.56) ml/(min·100 g)；随肝纤维化程度加重,PVP、TLP持续性下降(P＜0.05),HAP总体略呈上升趋势.PVP值、TLP值与肝纤维化程度呈负相关(P＜0.01)；VEGF表达水平随着肝纤维化程度的加重而明显增高(P＜0.05).结论腹腔注射四氯化碳油溶液辅以高脂饮食可以成功模拟人类从肝细胞变性、肝纤维化至肝硬化的全过程.64排螺旋CT全肝灌注成像克服了以往灌注成像的不足,且能反映肝纤维化各期的血流动力学变化趋势,VEGF可能在慢性肝病所致肝纤维化过程中起重要作用.
목적 이용64배라선CT전간관주모식성상측정중화전완견간섬유화모형적상관관주삼수병여병리대조래반영간섬유화정도,분석기여VEGF표체수평적상관성.방법 채용복강주사50％사록화탄유용액보이고지음식,건립견간섬유화모형,정기행64배라선CT전간관주성상급간천활검.근거병리분기분석각조CT관주삼수,병여VEGF표체수평작대조연구.결과 성공획득각기간섬유화모형.대조조HAP(28.25 ±2.19) ml/(min·100 g),PVP( 53.53±10.71) ml/( min ·100 g),TLP(81.78±18.56) ml/(min·100 g)；수간섬유화정도가중,PVP、TLP지속성하강(P＜0.05),HAP총체략정상승추세.PVP치、TLP치여간섬유화정도정부상관(P＜0.01)；VEGF표체수평수착간섬유화정도적가중이명현증고(P＜0.05).결론 복강주사사록화탄유용액보이고지음식가이성공모의인류종간세포변성、간섬유화지간경화적전과정.64배라선CT전간관주성상극복료이왕관주성상적불족,차능반영간섬유화각기적혈류동역학변화추세,VEGF가능재만성간병소치간섬유화과정중기중요작용.
Objective To measure the perfusion parameters of liver fibrosis of dog model with 64-slice spiral CT,and compare with positive control to reflect the degree of liver fibrosis,and analyze the correlation with VEGF values.Methods Liver fibrosis was induced in dogs by intraperitoneal injection of CC14 and high fat diet.CT perfusion scan and liver biopsies were performed.The perfusion parameters were measured according to the liver fibrosis models,and the correlation with VEGF values was analyzed.Results The animals in experimental group were successfully induced different degree of liver fibrosis.In normal group,the hepatic artery perfusion,portal vein perfusion and total hepatic blood flow were (28.25 ±2.19)ml/(min · 100 g),(53.53 ± 10.71)ml/(min · 100 g) and (81.78 ± 18.56) ml/(min · 100 g).The PVP and TLP values of the liver fibrosis models of 0 - 4 stages declined gradually,and it had statistical significance,while the HAP values increased.The PVP and TLP values were positive correlated with fibrosis stage.The VEGF values of the pathological models of 0 - 4 stages ascended significantly.Conclusions The dog liver fibrosis models that are similar to human hepatic fibrosis have been successfully induced by low dose CC14 and high fat diet.CT perfusion can be used to monitor the tendency of the hemodynamic in different degree of fibrosis.VEGF may play an important role during liver fibrogenesis.