中华核医学与分子影像杂志
中華覈醫學與分子影像雜誌
중화핵의학여분자영상잡지
Chinese Journal of Nuclear Medicine and Molecular Imaging
2012年
2期
143-147
,共5页
王峰%任凌%李眉%张润武%刘黎洁%戴皓洁
王峰%任凌%李眉%張潤武%劉黎潔%戴皓潔
왕봉%임릉%리미%장윤무%류려길%대호길
喉肿瘤%鼻咽肿瘤%细胞粘着分子%放射性核素显像%小鼠,裸
喉腫瘤%鼻嚥腫瘤%細胞粘著分子%放射性覈素顯像%小鼠,裸
후종류%비인종류%세포점착분자%방사성핵소현상%소서,라
Laryngeal neoplasms%Nasopharyngeal neoplasms%Cell adhesion molecules%Radionuclide imaging%Mice,nude
目的 研究99Tcm标记的聚乙二醇(PEG)4修饰的环状RGD二聚体(99Tcm-3P-RGD2)显像用于检测人喉和鼻咽鳞状细胞癌(简称鳞癌)整合素αvβ3表达的可靠性.方法 对荷人HEP-2喉鳞癌、荷人CNE-1鼻咽鳞癌裸鼠各6只进行99Tcm-3P-RGD2平面显像,采用勾画ROI技术计算T/NT.显像结束后,测量99Tcm-3P-RGD2在荷瘤鼠体内的放射性分布,计算肿瘤与各组织器官的％ID/g.取肿瘤组织,行整合素αvβ3免疫组织化学染色,并参照Fromowitz法进行半定量分析.两组间比较采用独立样本t检验,相关性分析采用线性相关法.结果 荷HEP-2、CNE-1裸鼠2h显像时T/NT 分别为2.08±0.04与1.54±0.10.体内放射性分布示:HEP-2肿瘤2h放射性摄取值为(4.56±0.67)％ID/g,肿瘤与血液、肌肉的T/NT分别为6.37±0.68与4.44±0.42；CNE-1肿瘤2h放射性摄取值为(1.69 ±0.18) ％ID/g,肿瘤与血液、肌肉的T/NT分别为2.49±0.09与1.86±0.07.HEP-2、CNE-1肿瘤αvβ3免疫组织化学染色Fromowitz评分分别为4.97±0.37与2.60±0.36.荷HEP-2裸鼠2h显像时T/NT、％ID/g及免疫组织化学染色Fromowitz评分均显著高于荷CNE-1裸鼠(t值分别为11.83、7.17和11.31,P均＜0.05).2种荷瘤鼠2h显像时T/NT与免疫组织化学染色Fromowitz评分的相关性均较好(HEP-2:r2h =0.97,P＜0.05;CNE-1:r'2h =0.97,P＜0.05).结论 99Tcm-3P-RGD2显像有望成为检测喉和鼻咽鳞癌αvβ3表达的无创和有效方法.
目的 研究99Tcm標記的聚乙二醇(PEG)4脩飾的環狀RGD二聚體(99Tcm-3P-RGD2)顯像用于檢測人喉和鼻嚥鱗狀細胞癌(簡稱鱗癌)整閤素αvβ3錶達的可靠性.方法 對荷人HEP-2喉鱗癌、荷人CNE-1鼻嚥鱗癌裸鼠各6隻進行99Tcm-3P-RGD2平麵顯像,採用勾畫ROI技術計算T/NT.顯像結束後,測量99Tcm-3P-RGD2在荷瘤鼠體內的放射性分佈,計算腫瘤與各組織器官的％ID/g.取腫瘤組織,行整閤素αvβ3免疫組織化學染色,併參照Fromowitz法進行半定量分析.兩組間比較採用獨立樣本t檢驗,相關性分析採用線性相關法.結果 荷HEP-2、CNE-1裸鼠2h顯像時T/NT 分彆為2.08±0.04與1.54±0.10.體內放射性分佈示:HEP-2腫瘤2h放射性攝取值為(4.56±0.67)％ID/g,腫瘤與血液、肌肉的T/NT分彆為6.37±0.68與4.44±0.42；CNE-1腫瘤2h放射性攝取值為(1.69 ±0.18) ％ID/g,腫瘤與血液、肌肉的T/NT分彆為2.49±0.09與1.86±0.07.HEP-2、CNE-1腫瘤αvβ3免疫組織化學染色Fromowitz評分分彆為4.97±0.37與2.60±0.36.荷HEP-2裸鼠2h顯像時T/NT、％ID/g及免疫組織化學染色Fromowitz評分均顯著高于荷CNE-1裸鼠(t值分彆為11.83、7.17和11.31,P均＜0.05).2種荷瘤鼠2h顯像時T/NT與免疫組織化學染色Fromowitz評分的相關性均較好(HEP-2:r2h =0.97,P＜0.05;CNE-1:r'2h =0.97,P＜0.05).結論 99Tcm-3P-RGD2顯像有望成為檢測喉和鼻嚥鱗癌αvβ3錶達的無創和有效方法.
목적 연구99Tcm표기적취을이순(PEG)4수식적배상RGD이취체(99Tcm-3P-RGD2)현상용우검측인후화비인린상세포암(간칭린암)정합소αvβ3표체적가고성.방법 대하인HEP-2후린암、하인CNE-1비인린암라서각6지진행99Tcm-3P-RGD2평면현상,채용구화ROI기술계산T/NT.현상결속후,측량99Tcm-3P-RGD2재하류서체내적방사성분포,계산종류여각조직기관적％ID/g.취종류조직,행정합소αvβ3면역조직화학염색,병삼조Fromowitz법진행반정량분석.량조간비교채용독립양본t검험,상관성분석채용선성상관법.결과 하HEP-2、CNE-1라서2h현상시T/NT 분별위2.08±0.04여1.54±0.10.체내방사성분포시:HEP-2종류2h방사성섭취치위(4.56±0.67)％ID/g,종류여혈액、기육적T/NT분별위6.37±0.68여4.44±0.42；CNE-1종류2h방사성섭취치위(1.69 ±0.18) ％ID/g,종류여혈액、기육적T/NT분별위2.49±0.09여1.86±0.07.HEP-2、CNE-1종류αvβ3면역조직화학염색Fromowitz평분분별위4.97±0.37여2.60±0.36.하HEP-2라서2h현상시T/NT、％ID/g급면역조직화학염색Fromowitz평분균현저고우하CNE-1라서(t치분별위11.83、7.17화11.31,P균＜0.05).2충하류서2h현상시T/NT여면역조직화학염색Fromowitz평분적상관성균교호(HEP-2:r2h =0.97,P＜0.05;CNE-1:r'2h =0.97,P＜0.05).결론 99Tcm-3P-RGD2현상유망성위검측후화비인린암αvβ3표체적무창화유효방법.
Objective To study the reliability of99Tcm-poly-(ethyleneglycol)(PEG)4-E(PEG4-c(RGDfK) )2 (99Tcm-3P-RGD2) as a radiotracer for integrin αvβ3 expression in laryngeal and nasopharyngeal squamous cell carcinoma in nude mice.Methods Twelve nude mice bearing human HEP-2 laryngeal carcinoma xenograft (n =6) and human CNE-1 nasopharyngeal carcinoma xenograft (n =6) were used for 99Tcm-3P-RGD2 planar imaging,and T/NT ratios were obtained with ROI technique.Afterwards,the biodistribution of 99Tcm-3P-RGD2 was analyzed in tumor beating nude mice,and the radioactivity in tumor and other organs (％ ID/g) were calculated.The immunostaining was performed to examine integrin αvβ3 expression in tumor tissue and semi-quantitative analysis was done according to the Fromowitz method.The independent samples t-test and linear correlation analysis were also used.Results The T/NT ratios of HEP-2 and CNE-1 bearing nude mice were 2.08 ±0.04 and 1.54 ±0.10 at 2 h post-injection,respectively.The radioactivity was (4.56 ± 0.67) ％ ID/g in HEP-2 tumor and ( 1.69 ± 0.18) ％ ID/g in CNE-1 tumor at 2 h post-injection.The T/NT ratios of tumor to blood and tumor to muscle were 6.37 ± 0.68 and 4.44 ± 0.42 in HEP-2 bearing nude mice,and 2.49 ±0.09 and 1.86 ±0.07 in CNE-1 bearing nude mice,respectively.The Fromowitz scores were 4.97 ± 0.37 and 2.60 ± 0.36 in HEP-2 and CNE-1 tumors,respectively.The T/NT ratio,the amount of radioactivity in tumor at 2 h post-injection and the Fromowitz score of HEP-2 bearing nude mice were all significantly higher than those of CNE-1 bearing nude mice (t =11.83,7.17 and 11.31respectively,all P ＜ 0.05 ).The T/NT ratios at 2 h post-injection were correlated well with Fromowitz scores (HEP-2:r2h =0.97,P＜0.05; CNE-1:r'2h =0.97,P＜0.05).Conclusion 99Tcm-3P-RGD2 imaging could be a noninvasive and effective method for monitoring integrin αv β3 expression in laryngeal and nasopharyngeal squamous cell carcinoma.
