CAJ | 학술논문

目的探讨监测乳腺癌患者外周血中CK19 mRNA表达浓度的临床意义. 方法用实时定量PCR法测定137例乳腺癌患者术前、术后第1天、术后第7天、术后1个月、术后3个月、术后6个月、术后12个月、术后18个月及术后24个月等各时间点的外周血中CK19 mRNA的表达,分析CK19浓度变化与乳腺癌复发转移之间的关系.结果 ①术前、术后第1天、术后第7天3个时间点乳腺癌患者外周血的CK19 mRNA的浓度无明显变化(P＞0.05)；术后1个月CK19 mRNA水平较术前、术后第1天及术后第7天相比均有明显下降(P＜0.05).未复发组的外周血中CK19 mRNA浓度持续维持在低水平,而复发转移组的CK19 mRNA表达浓度在下降后再次升高(P＜0.01).②CK19 mRNA的表达与月经状态、雌激素受体、孕激素受体、HER2和Ki67表达无明确相关(P＞0.05)；与肿瘤大小、肿瘤组织学分级、病理类型及淋巴结转移等指标均有明显相关性(P＜0.01).结论乳腺癌患者外周血CK19 mRNA的表达与临床病理高危因素指标相关,复发转移时其浓度升高,可能成为乳腺癌患者复发转移的指标.
목적 탐토감측유선암환자외주혈중CK19 mRNA표체농도적림상의의. 방법 용실시정량PCR법측정137례유선암환자술전、술후제1천、술후제7천、술후1개월、술후3개월、술후6개월、술후12개월、술후18개월급술후24개월등각시간점적외주혈중CK19 mRNA적표체,분석CK19농도변화여유선암복발전이지간적관계.결과 ①술전、술후제1천、술후제7천3개시간점유선암환자외주혈적CK19 mRNA적농도무명현변화(P＞0.05)；술후1개월CK19 mRNA수평교술전、술후제1천급술후제7천상비균유명현하강(P＜0.05).미복발조적외주혈중CK19 mRNA농도지속유지재저수평,이복발전이조적CK19 mRNA표체농도재하강후재차승고(P＜0.01).②CK19 mRNA적표체여월경상태、자격소수체、잉격소수체、HER2화Ki67표체무명학상관(P＞0.05)；여종류대소、종류조직학분급、병리류형급림파결전이등지표균유명현상관성(P＜0.01).결론 유선암환자외주혈CK19 mRNA적표체여림상병리고위인소지표상관,복발전이시기농도승고,가능성위유선암환자복발전이적지표.
Objective To evaluate the significance of CK19 mRNA monitoring in peripheral blood of breast cancer patients. Methods Peripheral blood samples were collected from 137 breast cancer patients preoperatively,one day post-operation,7 days,1,3,6,12,18,and 24 months after operation.RTPCR was used to detect CK19 mRNA expression.The relationships were analyzed between CK19 mRNA expression and treatment result, between CK19 mRNA expression and clinicopathological parameters.Results There was no significant difference between the level of CK19 mRNA as tested preoperatively,and 1,7 days postoperatively(P ＞ 0.05 ).From one month and thereafter,CK 19 mRNA decreased significantly when compared with that immediate perioperatives ( P ＜ 0.05 ). Postoperative peripheral CK19 mRNA expression increased in those found with recurrent or metastasized tumors ( P ＜ 0.01 ). CK19 mRNA expression does not correlate with patient's menstrual status,estrogen receptor expression,progesterone receptor expression,HER-2 expression and Ki67 proliferation expression (P ＞ 0.05 ). But there was statistically significant correlation between CK19 mRNA expression and tumour size,histology grading,pathological type,lymph node metastasis ( P ＜ 0.01 ). Conclusions In breast cancer patients peripheral blood CK19 mRNA expression is correlated with risk clinicopathological parameters, and increases in patients with postoperative recurrence and tumor metastasis.