中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2008年
10期
756-759
,共4页
包俊炜%吴河水%王春友%张进祥%张景辉%田元%徐颖
包俊煒%吳河水%王春友%張進祥%張景輝%田元%徐穎
포준위%오하수%왕춘우%장진상%장경휘%전원%서영
胰腺炎,急性坏死性%Toll样受体4%内毒素类%移植嵌合体
胰腺炎,急性壞死性%Toll樣受體4%內毒素類%移植嵌閤體
이선염,급성배사성%Toll양수체4%내독소류%이식감합체
Pancreatitis,acute necrotizing%Toll-like receptor 4%Endotoxins%Transplantation chimera
目的 探索髓系细胞(中性粒细胞、血小板)及内皮细胞表面TLR4表达在重症急性胰腺炎中性粒细胞招募中的作用.方法 采用C3H/He-J和C3H/He-N小鼠通过骨髓移植的方法 制作"髓系细胞TLR4 -/- 和内皮细胞TLR4 +/+ "(A组)及"髓系细胞TLR4 +/+ 和内皮细胞TLR4 -/- "(B组)嵌合体小鼠及"髓系细胞TLR4 +/+ 和内皮细胞TLR4 +/+ "(C组)及"髓系细胞TLR4 -/- 和内皮细胞TLR4 -/- "(D组)纯合体小鼠,腹腔注射蛙皮素、尾静脉注射脂多糖复制SAP模型,检测血浆淀粉酶、PCR测外周血粒细胞TLR4、免疫组化检测胰腺组织内TLR4的表达、胰腺组织萘酚AS-D氯乙酸脂酶染色计数、MPO活性.结果 各实验组小鼠血浆淀粉酶升高;B、C两组外周血粒细胞TLR4mRNA表达高于A、D两组(P<0.05);A、C两组胰腺组织内TLR4阳性,B、D两组阴性;A、C两组AS-D计数高于B、D两组;A、C两组MPO活性亦明显高于B、D两组(P<0.05).结论 内皮细胞而非外周血粒细胞在重症急性胰腺炎中性粒细胞招募聚集中起关键作用.
目的 探索髓繫細胞(中性粒細胞、血小闆)及內皮細胞錶麵TLR4錶達在重癥急性胰腺炎中性粒細胞招募中的作用.方法 採用C3H/He-J和C3H/He-N小鼠通過骨髓移植的方法 製作"髓繫細胞TLR4 -/- 和內皮細胞TLR4 +/+ "(A組)及"髓繫細胞TLR4 +/+ 和內皮細胞TLR4 -/- "(B組)嵌閤體小鼠及"髓繫細胞TLR4 +/+ 和內皮細胞TLR4 +/+ "(C組)及"髓繫細胞TLR4 -/- 和內皮細胞TLR4 -/- "(D組)純閤體小鼠,腹腔註射蛙皮素、尾靜脈註射脂多糖複製SAP模型,檢測血漿澱粉酶、PCR測外週血粒細胞TLR4、免疫組化檢測胰腺組織內TLR4的錶達、胰腺組織萘酚AS-D氯乙痠脂酶染色計數、MPO活性.結果 各實驗組小鼠血漿澱粉酶升高;B、C兩組外週血粒細胞TLR4mRNA錶達高于A、D兩組(P<0.05);A、C兩組胰腺組織內TLR4暘性,B、D兩組陰性;A、C兩組AS-D計數高于B、D兩組;A、C兩組MPO活性亦明顯高于B、D兩組(P<0.05).結論 內皮細胞而非外週血粒細胞在重癥急性胰腺炎中性粒細胞招募聚集中起關鍵作用.
목적 탐색수계세포(중성립세포、혈소판)급내피세포표면TLR4표체재중증급성이선염중성립세포초모중적작용.방법 채용C3H/He-J화C3H/He-N소서통과골수이식적방법 제작"수계세포TLR4 -/- 화내피세포TLR4 +/+ "(A조)급"수계세포TLR4 +/+ 화내피세포TLR4 -/- "(B조)감합체소서급"수계세포TLR4 +/+ 화내피세포TLR4 +/+ "(C조)급"수계세포TLR4 -/- 화내피세포TLR4 -/- "(D조)순합체소서,복강주사와피소、미정맥주사지다당복제SAP모형,검측혈장정분매、PCR측외주혈립세포TLR4、면역조화검측이선조직내TLR4적표체、이선조직내분AS-D록을산지매염색계수、MPO활성.결과 각실험조소서혈장정분매승고;B、C량조외주혈립세포TLR4mRNA표체고우A、D량조(P<0.05);A、C량조이선조직내TLR4양성,B、D량조음성;A、C량조AS-D계수고우B、D량조;A、C량조MPO활성역명현고우B、D량조(P<0.05).결론 내피세포이비외주혈립세포재중증급성이선염중성립세포초모취집중기관건작용.
Objective To investigate the significance of expression of toll-like receptor on medullary system cell and endothelial cell surface in PMN recruiting in severe acute pancreatitis.Method In this study,26 C3H/He-J mice and 26 C3H/He-N mice were derided into 4 groups by bone marrow transplantation:group A, mosaic of medullary system cell TLR4-/- and endothelial cell TLR4+/+,group B, mosaic of medullary system cell TLR4+/+ and endothelial cell TLR4-/- ;group C, homozygote ofTLR4+/+ ;group D, homozygote of TLR4-/-. SAP was induced to all these mice. Amylase, TRIA of granular cell in peripheral blood,TRL4 expression in pancreas, Naphthol AS-D chloroacetate esterase, MPO activity were tested. Results Amylase level increased in all mice, there was no differences of the amylase level among groups, TRIA of granular cell in peripheral blood in group B and C was significant higher than A and D(P<0.05), TLR4 in pancreas were positive in group A and C, negative in group B and D, AS-D count and M PO activity in group A and C were higher than that in group B and D (P < 0. 05 ). Conclusions It was endothelial cell, not peripheral blood granulocyte, that plays a key role in recuiting neutrophils that triggers severe acute pancreatitis.