CAJ | 학술논문

目的检测乳腺丝氨酸蛋白酶抑制剂(mammary serine proteinase inhibitor,Maspin)在口腔鳞状细胞癌亚细胞中的表达,观察其与口腔鳞状细胞癌患者临床病理特征之间的关系,以期为临床提供参考.方法应用免疫组织化学方法对45例口腔鳞状细胞癌的癌组织标本行Maspin蛋白含量的半定量测定,统计其亚细胞定位表达,并与各临床病理指标进行统计学分析.结果 Maspin蛋白在口腔鳞状细胞癌胞核中表达的强阳性率为24%(11/45),弱阳性率为11%(5/45),阴性率为64%(29/45),细胞核表达与肿瘤大小(P=0.019)、淋巴结转移(P=0.011)及术后转移(P=0.017)呈负相关,与患者术后的生存时间呈正相关(P=0.030);Maspin蛋白在口腔鳞状细胞癌胞质中表达的强阳性率为31%(14/45),弱阳性率为31%(14/45),阴性率为38%(17/45),细胞质表达与淋巴结转移(P=0.038)、术后转移(P=0.004)呈负相关,与患者术后的生存时间呈正相关(P=0.014).结论 Maspin蛋白在口腔鳞状细胞癌胞核或胞质中的表达对判断预后可能具有重要价值.
목적 검측유선사안산단백매억제제(mammary serine proteinase inhibitor,Maspin)재구강린상세포암아세포중적표체,관찰기여구강린상세포암환자림상병리특정지간적관계,이기위림상제공삼고.방법 응용면역조직화학방법대45례구강린상세포암적암조직표본행Maspin단백함량적반정량측정,통계기아세포정위표체,병여각림상병리지표진행통계학분석.결과 Maspin단백재구강린상세포암포핵중표체적강양성솔위24%(11/45),약양성솔위11%(5/45),음성솔위64%(29/45),세포핵표체여종류대소(P=0.019)、림파결전이(P=0.011)급술후전이(P=0.017)정부상관,여환자술후적생존시간정정상관(P=0.030);Maspin단백재구강린상세포암포질중표체적강양성솔위31%(14/45),약양성솔위31%(14/45),음성솔위38%(17/45),세포질표체여림파결전이(P=0.038)、술후전이(P=0.004)정부상관,여환자술후적생존시간정정상관(P=0.014).결론 Maspin단백재구강린상세포암포핵혹포질중적표체대판단예후가능구유중요개치.
Objective To investigate the subcellular expression of mammary serine proteinase inhibitor(Maspin) in oral squamous cell carcinoma and its relationship to the clinicopathological features.Methods The Maspin protein subcellular expression was detected in 45 patients with oral squamous cell carcinoma by immunohistochemical staining.The relationship between the Maspin protein subcellular expression and the clinicopathological parameters was analyzed.Results The negative rate of nuclear maspin expression was 64%(29/45),and the weakly positive rate was 11%(5/45),and the strong positive rate was 24%(11/45).Nuclear maspin expression was negatively correlated with T stage(P=0.019),lymph node metastasis(P=0.038) and postoperative metastasis(P=0.004),but positively correlated with the patients′ survival rate (P=0.014).The negative rate of cytoplasmatic maspin expression was 38%(17/45),and the weakly positive rate was 31%(14/45),and the strong positive rate was 31%(14/45).Cytoplasmatic maspin expression was negatively correlated with lymph node metastasis(P=0.038) and postoperative metastasis(P=0.004),but positively correlated with the patients′ survival rate (P=0.014).Conclusions Maspin expression may be a significant marker in predicting prognosis of oral squamous cell carcinoma.