中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2008年
6期
723-724
,共2页
骨肉瘤%肿瘤坏死因子%基因多态性
骨肉瘤%腫瘤壞死因子%基因多態性
골육류%종류배사인자%기인다태성
Osteosarcoma%Tumor necrosis factor%Gene polymorphisms
目的 观察骨肉瘤患者肿瘤坏死因子 (TNF)基因多态性,探讨骨肉瘤与TNF基因多态性的相关性.方法 应用聚合酶链反应-限制性片段长度多态性分析方法(PCR-RFLP) 对52例骨肉瘤患者与60例健康对照者TNF-α和TNFβ基因的NcoI酶切位点进行对比分析.结果 52例骨肉瘤患者TNF-α- 308位点G/G基因型频率(98%) 及G等位基因频率(99%) 显著高于正常对照组G/G基因型频率85.0%及G等位基因频率91.7%(P<0.05),TNF-β+252的基因型频率和等位基因频率与正常对照人群比较差异无统计学意义(P>0.05). 结论 TNFα基因多态性与骨肉瘤的发生具有相关性,TNFβ基因多态性与骨肉瘤的发生无明显相关.
目的 觀察骨肉瘤患者腫瘤壞死因子 (TNF)基因多態性,探討骨肉瘤與TNF基因多態性的相關性.方法 應用聚閤酶鏈反應-限製性片段長度多態性分析方法(PCR-RFLP) 對52例骨肉瘤患者與60例健康對照者TNF-α和TNFβ基因的NcoI酶切位點進行對比分析.結果 52例骨肉瘤患者TNF-α- 308位點G/G基因型頻率(98%) 及G等位基因頻率(99%) 顯著高于正常對照組G/G基因型頻率85.0%及G等位基因頻率91.7%(P<0.05),TNF-β+252的基因型頻率和等位基因頻率與正常對照人群比較差異無統計學意義(P>0.05). 結論 TNFα基因多態性與骨肉瘤的髮生具有相關性,TNFβ基因多態性與骨肉瘤的髮生無明顯相關.
목적 관찰골육류환자종류배사인자 (TNF)기인다태성,탐토골육류여TNF기인다태성적상관성.방법 응용취합매련반응-한제성편단장도다태성분석방법(PCR-RFLP) 대52례골육류환자여60례건강대조자TNF-α화TNFβ기인적NcoI매절위점진행대비분석.결과 52례골육류환자TNF-α- 308위점G/G기인형빈솔(98%) 급G등위기인빈솔(99%) 현저고우정상대조조G/G기인형빈솔85.0%급G등위기인빈솔91.7%(P<0.05),TNF-β+252적기인형빈솔화등위기인빈솔여정상대조인군비교차이무통계학의의(P>0.05). 결론 TNFα기인다태성여골육류적발생구유상관성,TNFβ기인다태성여골육류적발생무명현상관.
Objective To investigate the relationship between polymorphism of TNF gene and osteosarcoma.Methods A Nco1 restriction site polymorphism in the TNF-α and TNF-β gene was analyzed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) in 52 patients with osteosarcoma and 60 healthy controls.Results The genotype frequency and allelic frequency of TNF-α - 308 in osteosarcoma patients ( 98% and 99%,respectively) were significantly higher than in control group (85.0% and91.7% respectively,P<0.05).There was no significant difference in genotype frequency and allelic frequency of TNF-β+252 between osteosarcoma patients and normal controls (P>0.05).Conclusion TNF-α- 308 polymorphism may be related with osteosarcoma susceptibility;No correlation was found between TNF-β+252 polymorphism and osteosarcoma.
