CAJ | 학술논문

目的探索去除肾上腺素能交感神经支配后大鼠良性增生前列腺的组织、细胞病理学变化.方法 30周龄雄性自发性高血压大鼠65只,随机分配为手术组、手术对照组和正常对照组.手术组切断双侧盆腔主要神经节的交感腹下神经来源支,然后行膀胱造口术;手术对照组仅行膀胱造口术.分别于术后3、7、11、15、≥21 d分批处死大鼠,观察各组大鼠前列腺大体形态学、组织学和细胞超微结构改变.结果大鼠实验模型均制作成功.手术组大鼠前列腺在后期(手术15 d后)出现轻微的质地变硬,颜色和形状变化不明显,前列腺湿重/大鼠体重比值随术后时间的延长有下降趋势,前列腺组织干重/湿重比值在手术15 d后逐渐升高,但与手术对照组比较差异均无统计学意义.镜下观:手术组大鼠前列腺早期出现腺腔明显扩张、前列腺液积存和凝块形成,平滑肌拉长变薄,但后期病变逐步缓解,腺上皮层无变化.透射电镜:手术组大鼠前列腺腺细胞、基底膜无明显变化,但平滑肌术后3 d出现肌膜皱缩,肌丝、密体和密斑结构不清晰等改变,术后11 d后,病变逐步好转.手术对照组无上述变化.结论彻底去除肾上腺素能交感神经支配后,大鼠良性增生前列腺的平滑肌出现短时间的结构受损和功能障碍,后期能自行部分恢复.
목적 탐색거제신상선소능교감신경지배후대서량성증생전렬선적조직、세포병이학변화.방법 30주령웅성자발성고혈압대서65지,수궤분배위수술조、수술대조조화정상대조조.수술조절단쌍측분강주요신경절적교감복하신경래원지,연후행방광조구술;수술대조조부행방광조구술.분별우술후3、7、11、15、≥21 d분비처사대서,관찰각조대서전렬선대체형태학、조직학화세포초미결구개변.결과 대서실험모형균제작성공.수술조대서전렬선재후기(수술15 d후)출현경미적질지변경,안색화형상변화불명현,전렬선습중/대서체중비치수술후시간적연장유하강추세,전렬선조직간중/습중비치재수술15 d후축점승고,단여수술대조조비교차이균무통계학의의.경하관:수술조대서전렬선조기출현선강명현확장、전렬선액적존화응괴형성,평활기랍장변박,단후기병변축보완해,선상피층무변화.투사전경:수술조대서전렬선선세포、기저막무명현변화,단평활기술후3 d출현기막추축,기사、밀체화밀반결구불청석등개변,술후11 d후,병변축보호전.수술대조조무상술변화.결론 철저거제신상선소능교감신경지배후,대서량성증생전렬선적평활기출현단시간적결구수손화공능장애,후기능자행부분회복.
Objective To study the pathologic change of rats' benign hyperplastic prostate after losing the innervation of sympathetic nerve. Methods A total of 65 male spontaneous hypertension rats (SHR) aged 30 weeks old were randomly assigned into treatment group, sham surgery control group and normal control group. In treatment group, the originating branches of sympathetic hypngastric nerve of the bilateral major pelvic ganglions were truncated following the performance of eystostomy. The rata were sac-rificed at day 3,7,11,15 and≥21 post-operation respectively. The gross morphological changes, histologi-cal changes and cellular ultra-structural changes of prostate in all animals were studied. Results In treat-ment group,the prostates became slightly harder in consistency at day 15 post-operation, with no marked changes in color and morphology. H&E staining showed that the glandular lumen became dilated and rounded ,with accumulation of prostatic fluid and condensed mass formation, the smooth muscles elongated and thinned, but these pathologic changes improved gradually in later stage, and glandular epithelia were normal. Electron microscopy revealed that mysiurn in smooth muscle cells became crumpled and the strue-tunes of myofilament,dense body and dense spot were indistinct 3 days after operation, and these changes amended gradually at day 11 post-operation, no marked changes were found in glandular cells. All these abnormalities were not obtained in sham surgery control group. Conclusion After losing the innervation of sympathetic nerve ,the smooth muscle in rata' benign hyperplastic prostate underwent destruction of structure and function, but the destruction was improved gradually in the later stage.