CAJ | 학술논문

目的检测冠状动脉粥样硬化斑块中的5种特异性牙周致病菌.方法收集101例行冠状动脉搭桥手术患者的动脉粥样硬化斑块标本,采用Chelex-100法提取冠状动脉粥样硬化斑块中的DNA,并通过PCR分别检测动脉粥样硬化斑块中的牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)、伴放线放线杆菌(Actinobacillus actinomycetemcomitans,Aa)、具核梭杆菌(Fusobacterium nucleatum,Fn)、中间普氏菌(Prevotella intermedia,Pi)、福赛斯坦纳菌(Tannerella forsythensis,Tf)5种牙周特异性致病菌.结果 101例患者动脉粥样硬化斑块中牙龈卟啉单胞菌(Pg)的检出率为31％,福赛斯坦纳菌(Tf)为42％,中间普氏菌(Pi)为26％,具核梭杆菌(Fn)为21％,伴放线放线杆菌(Aa)为23％.这几种细菌的PCR产物通过测序,结果与GenBank数据库中的序列进行比对同源性达99％～100％.结论牙周致病菌在冠心病的发生、发展中可能发挥着一定作用,考虑慢性牙周炎与冠心病之间有一定的相关性.
목적 검측관상동맥죽양경화반괴중적5충특이성아주치병균.방법 수집101례행관상동맥탑교수술환자적동맥죽양경화반괴표본,채용Chelex-100법제취관상동맥죽양경화반괴중적DNA,병통과PCR분별검측동맥죽양경화반괴중적아간계람단포균(Porphyromonas gingivalis,Pg)、반방선방선간균(Actinobacillus actinomycetemcomitans,Aa)、구핵사간균(Fusobacterium nucleatum,Fn)、중간보씨균(Prevotella intermedia,Pi)、복새사탄납균(Tannerella forsythensis,Tf)5충아주특이성치병균.결과 101례환자동맥죽양경화반괴중아간계람단포균(Pg)적검출솔위31％,복새사탄납균(Tf)위42％,중간보씨균(Pi)위26％,구핵사간균(Fn)위21％,반방선방선간균(Aa)위23％.저궤충세균적PCR산물통과측서,결과여GenBank수거고중적서렬진행비대동원성체99％～100％.결론 아주치병균재관심병적발생、발전중가능발휘착일정작용,고필만성아주염여관심병지간유일정적상관성.
Objective To detect five periodontal pathogenic bacteria in coronary atherosclerotic plaques.Methods Atherosclerotic plaque specimens were obtained from 101 patients who scheduled for coronary artery bypass graft surgery.The bacteria DNA was obtained from coronary atherosclerotic plaques using the chelex-100 method.The extracted DNA were examined by PCR.Results Within the 101 pieces of atherosclerotic plaque samples Porphyromonas gingivalis( Pg,31％ ),Tannerella forsythensis(Tf,42％ ),Prevotella intermedia( Pi,26％ ),Fusobacterium nucleatum( Fn,21％ ),Actinobacillus actinomycetemcomitans( Aa,23％ ).PCR products were sequenced and were compared with GenBank sequences,the homology was 99％-100％.Conclusion Periodontitis might affect the development of atherosclerosis and there is a correlation between coronary heart disease and chronic periodontitis.