CAJ | 학술논문

目的探讨MMP-3基因单核苷酸多态性(SNP)与冠心病患者冠状动脉粥样硬化程度间的相关性.方法入选经冠状动脉造影证实的汉族冠心病患者1371例及健康对照695例,选择MMP-3-1612 5A/6A、-376C/G、Glu45Lys等SNPs位点,采用聚合酶链式反应-限制性内切酶片段长度多态性(PCR-RFLP)方法确定基因型.X2检验用于单因素分析时检验SNP位点与冠状动脉粥样硬化程度间的关联.结果 (1)-1612 5A/6A多态性位点在冠心病患者单支病变、双支病变、三支病变组中5A等位基因频率分别是0.185,0.183,0.152;-376C/G多态性位点在以上三组中-376G等位基因频率分别是0.329,0.326,0.325;Glu45Lys多态性位点在以上三组中45Lys等位基因频率分别是0.423,0.417,0.405.(2)-1612 5A/6A多态性位点5A等位基因频率在三支病变组中明显低于单支病变组(OR=0.74,P=0.04);相对于6A/6A基因型,含有5A等位基因的纯合子及杂合子频率在三支病变组明显低于单支病变组(OR=0.74,P=0.04).结论中国汉族冠心病患者中MMP-3基因-1612 5A/6A多态性位点可能与冠心病患者冠状动脉粥样硬化程度相关,5A等位基因可能对冠状动脉粥样硬化的进展有保护作用.
목적 탐토MMP-3기인단핵감산다태성(SNP)여관심병환자관상동맥죽양경화정도간적상관성.방법 입선경관상동맥조영증실적한족관심병환자1371례급건강대조695례,선택MMP-3-1612 5A/6A、-376C/G、Glu45Lys등SNPs위점,채용취합매련식반응-한제성내절매편단장도다태성(PCR-RFLP)방법학정기인형.X2검험용우단인소분석시검험SNP위점여관상동맥죽양경화정도간적관련.결과 (1)-1612 5A/6A다태성위점재관심병환자단지병변、쌍지병변、삼지병변조중5A등위기인빈솔분별시0.185,0.183,0.152;-376C/G다태성위점재이상삼조중-376G등위기인빈솔분별시0.329,0.326,0.325;Glu45Lys다태성위점재이상삼조중45Lys등위기인빈솔분별시0.423,0.417,0.405.(2)-1612 5A/6A다태성위점5A등위기인빈솔재삼지병변조중명현저우단지병변조(OR=0.74,P=0.04);상대우6A/6A기인형,함유5A등위기인적순합자급잡합자빈솔재삼지병변조명현저우단지병변조(OR=0.74,P=0.04).결론 중국한족관심병환자중MMP-3기인-1612 5A/6A다태성위점가능여관심병환자관상동맥죽양경화정도상관,5A등위기인가능대관상동맥죽양경화적진전유보호작용.
Objective To investigate the association between the severity of coronary arteries in patients with coionary artery disease and the single nucleotide polymorphisms of MMP-3 gene.Methods One thousand and three hundred seventy-one patients with coronary artery disease (CAD) diagnosed by cornary angiography and six hundred and ninety-five healthy controls without CAD were enrolled in this study.The SNPs of-1612 5A/6A,-376C/G,Glu45Lys of MMP-3 were genotyped by restriction fragment length polymorphism analysis (RFLP) in all subjects.Univariate analysis was applied to measure the asseeiation of the single nucleotide polymorphisms with the severity of coronary arteries.Results The minor allele frequency of-1612 5A/6A was 0.189,0.185,0.183 and 0.152 (P<0.05 VS.non-CAD control and single stenosis).the minor allele frequency of-376c/G Was 0.311,0.329,0.326 and 0.325,and the minor allele frequency of Glu45Lys was 0.367,0.423,0.417 and 0.405 in non-CAD control,CAD patients with single,two and three vessels stenosis,respectively.5A allele frequency is significant lower in the group with three vessels stenosis than in non.CAD control and CAD patients witll single vessel stenosis(OR.0.74.P=0.04).The 5A/5A and 5A/6A genotypes frequency is significant lower in the group with three vessels stenosis than in the non-CAD group and CAD patients with single vessel stenosis(OR:0.74,P=0.04).Conclusions The single nucleotide polymorphism of-1612 5A/6A of MMP-3 gene may be associated with the severity of coronary athcroslerosis in the Chinese Han patients with coronary artery disease.and the 5A allele misht therefore,play a protective role on the progression of coronary atherosclerosis.