CAJ | 학술논문

目的观察强直性脊柱炎(AS)患者外周血单核及T淋巴细胞中CD147的表达情况,初步探讨CD147在AS发病及疾病活动中的意义.方法入组AS患者、类风湿关节炎(RA)患者及健康对照者(HC)各30例,以流式细胞术分析比较各组间外周血单核及T淋巴细胞CD147表达的差异,并经逆转录-聚合酶链反应(RT-PCR)法检测比较其外周血单个核细胞(PBMC)CD147 mRNA表达的差异;进而将AS患者单核及T淋巴细胞CD147表达水平与常用的疾病活动性指标行相关分析.结果 AS、RA及HC组外周血单核细胞CD147的平均荧光强度(MF1)分别为213.5±37.8、228.7±49.7及163.6±44.8,T淋巴细胞CD147的MFI则分别为36.8±10.1、40.2±10.5及28.3±10.6,AS组单核及T淋巴细胞CD147的表达水平均略低于RA患者,但差异均无统计学意义(均P＞0.05),而AS与RA组单核及T淋巴细胞CD147的表达水平均高于HC组,差异均有统计学意义(均P＜0.05);AS与RA组PBMC中CD147 mRNA表达水平均高于HC组(P＜0.05),而AS与RA组间差异无统计学意义(P＞0.05);AS患者单核及T淋巴细胞CD147 MFI与其ESR及CRP均存在正相关(P＜0.05).结论 AS患者单核及T淋巴细胞中CD147的表达较正常升高,其水平与患者ESR及CRP存在正相关,提示CD147可能是AS患者疾病发生发展的重要影响因素.
목적 관찰강직성척주염(AS)환자외주혈단핵급T림파세포중CD147적표체정황,초보탐토CD147재AS발병급질병활동중적의의.방법 입조AS환자、류풍습관절염(RA)환자급건강대조자(HC)각30례,이류식세포술분석비교각조간외주혈단핵급T림파세포CD147표체적차이,병경역전록-취합매련반응(RT-PCR)법검측비교기외주혈단개핵세포(PBMC)CD147 mRNA표체적차이;진이장AS환자단핵급T림파세포CD147표체수평여상용적질병활동성지표행상관분석.결과 AS、RA급HC조외주혈단핵세포CD147적평균형광강도(MF1)분별위213.5±37.8、228.7±49.7급163.6±44.8,T림파세포CD147적MFI칙분별위36.8±10.1、40.2±10.5급28.3±10.6,AS조단핵급T림파세포CD147적표체수평균략저우RA환자,단차이균무통계학의의(균P＞0.05),이AS여RA조단핵급T림파세포CD147적표체수평균고우HC조,차이균유통계학의의(균P＜0.05);AS여RA조PBMC중CD147 mRNA표체수평균고우HC조(P＜0.05),이AS여RA조간차이무통계학의의(P＞0.05);AS환자단핵급T림파세포CD147 MFI여기ESR급CRP균존재정상관(P＜0.05).결론 AS환자단핵급T림파세포중CD147적표체교정상승고,기수평여환자ESR급CRP존재정상관,제시CD147가능시AS환자질병발생발전적중요영향인소.
Objective To investigate the roles of CD147 in the pathogenesis and development of ankylosing spondylitis(AS). Methods Flow cytometry was used to detect the expression levels of CD147 in peripheral monocytes and T lymphocytes of 30 AS patients, 30 rheumatoid arthritis(RA)patients and 30 healthy controls(HC). reverse transcription-polymerase chain reaction(RT-PCR)was used to evaluate the expression levels of CD147 mRNA in peripheral blood mononuclear cells(PBMC). Then the expression levels of CD147 were compared among the groups. And a correlation analysis was conducted between CD147 levels and disease activity indices of AS patients. Results The mean fluorescence intensities of CD147 in monocytes of AS, RA and HC group were 213.5 ± 37.8, 228.7 ±49.7 and 163.6 ±44.8, and in T lymphocytes 36. 8 ± 10. 1, 40. 2 ± 10. 5 and 28. 3 ± 10. 6 respectively. Both the expression levels of CD147 in monocytes and T lymphocytes of AS patients were slightly lower than those of RA patients. But the differences was not statistically significant(P ＞ 0. 05). Both the CD147 levels in monocytes and T lymphocytes of AS and RA group were significantly higher than those of HC group(P ＜ 0. 05). The expression levels of CD147 mRNA in PBMC of AS and RA group were significantly higher than those of HC group(P ＜ 0. 05)while no significant difference was found between AS and RA group(P ＞ 0. 05). Both the expression levels of CD147 in monocytes and T lymphocytes of AS patients were positively correlated with the patients' erythrocyte sedimentation rate(ESR)and C-reactive protein(CRP). Conclusion The expressions of CD147 in peripheral monocytes and T lymphocytes of AS patients are up-regulated and their levels are positively correlated with patients' ESR and CRP. It implies that CD147 plays critical roles in the pathogenesis and development of AS.