CAJ | 학술논문

目的：证实人牙乳头细胞中BMP-7的表达，获得hBMP-7成熟肽基因。方法：提取人牙乳头细胞总RNA，反转录合成cDNA，以特异性引物扩增hBMP-7成熟肽基因并克隆入T载体，筛选阳性克隆进行序列测定。结果：PCR扩增得到一特异性的约430bp的片段，克隆后筛选出阳性克隆，序列测定结果表明与国外已发表的序列完全一致。结论：人牙乳头细胞中有BMP-7基因的表达，并克隆得到hBMP-7成熟肽基因，为hBMP-7在牙齿发育中的作用及其功能研究奠定了基础。
목적：증실인아유두세포중BMP-7적표체，획득hBMP-7성숙태기인。방법：제취인아유두세포총RNA，반전록합성cDNA，이특이성인물확증hBMP-7성숙태기인병극륭입T재체，사선양성극륭진행서렬측정。결과：PCR확증득도일특이성적약430bp적편단，극륭후사선출양성극륭，서렬측정결과표명여국외이발표적서렬완전일치。결론：인아유두세포중유BMP-7기인적표체，병극륭득도hBMP-7성숙태기인，위hBMP-7재아치발육중적작용급기공능연구전정료기출。
Objective:To clone and sequence the gene encoding hBMP-7 mature peptide from human dental papilla mesenchymal cells. Methods: Total RNA was extracted from cultured human dental papilla mesenchymal cells and the desired cDNA fragment was obtained by RT-PCR with two specific primers. The segment was inserted into T vector and the result plasmid was transformed into DH5α.The positive clone was sequenced.Results:A 430 bp specific fragment was obtained.The sequence of the gene was consistent with that encoding hBMP-7 mature peptide in the published references. Conclusion: The gene encoding hBMP-7 mature peptide exists in human dental papilla mesenchymal cells.