中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2004年
36期
8387-8389
,共3页
刘兴茂%陈昭烈%刘红%吴本传%熊福银%胥照平
劉興茂%陳昭烈%劉紅%吳本傳%熊福銀%胥照平
류흥무%진소렬%류홍%오본전%웅복은%서조평
生物医学工程%心肌%胶原%支架
生物醫學工程%心肌%膠原%支架
생물의학공정%심기%효원%지가
背景:目前已有转化的SV40细胞系、C2C12成肌细胞、心肌细胞、骨骼肌细胞以及胚胎干细胞用于修复哺乳动物心脏的细胞移植治疗,然而细胞移植治疗对严重缺失或损伤心肌结构的治疗的作用却不大.组织工程技术为此类心肌疾病的研究及治疗提供了新的可能性,目前已有可替代和改善骨、软骨、肾、肝、神经及皮肤等组织和器官的生物合成结构物.目的:探索多孔胶原作为构建三维工程心肌组织支架材料的可行性.设计:非随机、对照的实验研究.地点和对象:实验地点:军事医学科学院生物工程研究所.动物:清洁级1日龄Wistar大鼠400~500只,雌雄不限,体质量8~10 g;成年Wistar大鼠五六只,雌雄不限,体质量250 g,由军事医学科学院实验动物中心提供,饲养温度22~26℃,湿度40%~60%.干预:在无菌条件下,将经胰蛋白酶消化获得的新生鼠心肌细胞分别接种于三维多孔胶原支架和培养板上,比较心肌细胞在两种培养模式的代谢差异.主要观察指标:检测葡萄糖比消耗率、乳酸比产率、乳酸转化率、肌酸激酶及乳酸脱氢酶活力变化.结果:培养于多孔胶原支架上的心肌细胞的代谢特性近似于心肌细胞在培养板上培养的二维生长模式.结论:心肌细胞与多孔胶原支架形成了具有自律性同步收缩的三维工程心肌组织,多孔胶原作为心肌组织工程的支架材料有良好的应用前景.
揹景:目前已有轉化的SV40細胞繫、C2C12成肌細胞、心肌細胞、骨骼肌細胞以及胚胎榦細胞用于脩複哺乳動物心髒的細胞移植治療,然而細胞移植治療對嚴重缺失或損傷心肌結構的治療的作用卻不大.組織工程技術為此類心肌疾病的研究及治療提供瞭新的可能性,目前已有可替代和改善骨、軟骨、腎、肝、神經及皮膚等組織和器官的生物閤成結構物.目的:探索多孔膠原作為構建三維工程心肌組織支架材料的可行性.設計:非隨機、對照的實驗研究.地點和對象:實驗地點:軍事醫學科學院生物工程研究所.動物:清潔級1日齡Wistar大鼠400~500隻,雌雄不限,體質量8~10 g;成年Wistar大鼠五六隻,雌雄不限,體質量250 g,由軍事醫學科學院實驗動物中心提供,飼養溫度22~26℃,濕度40%~60%.榦預:在無菌條件下,將經胰蛋白酶消化穫得的新生鼠心肌細胞分彆接種于三維多孔膠原支架和培養闆上,比較心肌細胞在兩種培養模式的代謝差異.主要觀察指標:檢測葡萄糖比消耗率、乳痠比產率、乳痠轉化率、肌痠激酶及乳痠脫氫酶活力變化.結果:培養于多孔膠原支架上的心肌細胞的代謝特性近似于心肌細胞在培養闆上培養的二維生長模式.結論:心肌細胞與多孔膠原支架形成瞭具有自律性同步收縮的三維工程心肌組織,多孔膠原作為心肌組織工程的支架材料有良好的應用前景.
배경:목전이유전화적SV40세포계、C2C12성기세포、심기세포、골격기세포이급배태간세포용우수복포유동물심장적세포이식치료,연이세포이식치료대엄중결실혹손상심기결구적치료적작용각불대.조직공정기술위차류심기질병적연구급치료제공료신적가능성,목전이유가체대화개선골、연골、신、간、신경급피부등조직화기관적생물합성결구물.목적:탐색다공효원작위구건삼유공정심기조직지가재료적가행성.설계:비수궤、대조적실험연구.지점화대상:실험지점:군사의학과학원생물공정연구소.동물:청길급1일령Wistar대서400~500지,자웅불한,체질량8~10 g;성년Wistar대서오륙지,자웅불한,체질량250 g,유군사의학과학원실험동물중심제공,사양온도22~26℃,습도40%~60%.간예:재무균조건하,장경이단백매소화획득적신생서심기세포분별접충우삼유다공효원지가화배양판상,비교심기세포재량충배양모식적대사차이.주요관찰지표:검측포도당비소모솔、유산비산솔、유산전화솔、기산격매급유산탈경매활력변화.결과:배양우다공효원지가상적심기세포적대사특성근사우심기세포재배양판상배양적이유생장모식.결론:심기세포여다공효원지가형성료구유자률성동보수축적삼유공정심기조직,다공효원작위심기조직공정적지가재료유량호적응용전경.
BACKGROUND: There have been a number of cell transplantation studies in which cells of various types, including SV40-transformed cell lines, C2C12 myoblasts, isolated cardiomyocytes, skeletal myoblasts, and embryonic stem cells, have been implanted into mammalian hearts. However, the effect of cell transplantation may be of little benefit in cases where the local cardiac structure is missing or seriously damaged. Tissue engineering offers the possibility of creating functional tissue equivalent for scientific studies and tissue repair. Biosynthetic constructs have been described that would replace or augment bone, cartilage, kidney, liver, neuronal tissue and skin.OBJECTIVE: To explore feasibility of porous collagen as three-dimensional cardiac scaffolds for tissue architecture.DESIGN: A non-randomized controlled experimental study.SETTING and PARTICIPANTS: The study was completed in the Institute of Biotechnology, Academy of Military Medical Sciences. Subjects were neonatal Wistar rats(1 day old, 8 - 10 g, clean grade) and adult Wistar (250 g) from the Laboratory Animal Center, Academy of Military Medical Sciences. Temperature and humidity of breeding were 22 - 26 ℃ and 40%- 60% respectively.INTERVENTIONS: Cardiac cells enzymatically digested from neonatal rats were seeded to three-dimensional porous collagen scaffolds and plastic plates. Metabolic characteristics of cardiac cells cultured between two culture modes were compared.MAIN OUTCOME MEASURES: The specific consumption rate of glucose(qg1c), specific production rate of lactate(q1ac), lactate transform rate(Y1aac/g1c), creatine kinase(CK) and lactate dehydrogenase(LDH) activities of cardiac cells.RESULTS: The metabolic characteristic of cardiac cells cultured in collagen scaffolds was similar to that cultured in polystyrene plates in two-dimensional mode.CONCLUSION: Synchronous and rhythmical contractile tissue constructs in vitro can be formed by cultivation of cardiac cells on porous collagen scaffolds. Porous collagen can be used as scaffolds in cardiac tissue engineering.