CAJ | 학술논문

背景:肺的功能和结构改变致肺动脉高压是导致肺心病的先决条件,中药肺心合剂能够有效降低肺动脉高压,但其机制尚未完全阐明.目的:比较不同剂量中药肺心合剂与硝苯地平对肺动脉高压大鼠肺血管重建的逆转效应.设计:以实验动物为研究对象的随机对照实验研究.单位:一所医科大学附属医院护理系、一所医学院附属医院、一所省级医院中医科.方法:利用野百合碱(50 mg/kg)复制大鼠肺心病模型.雄性Wister大鼠60只,随机分为6组:正常对照组、模型组、肺心合剂低、中、高剂量组及硝苯地平组,每组10只.分别于制模后14 d灌胃相应药物,连续用药8 d.处死动物后,利用特殊染色结合病理图象分析方法,测定肺小动脉病理及其形态计量学改变.主要观察指标:①光镜观察及肺小动脉图象分析.②各组肺小动脉管壁中膜厚度,管壁中膜厚度与血管外径比值、管壁面积与血管总面积比值、管腔面积与血管总面积比值.结果:肺心合剂及硝苯地平均能明显减轻模型大鼠的肺血管重构(P＜0.01),以肺心合剂高剂量组效果最好.但治疗组各指标仍未完全恢复到正常对照组水平.结论:肺心合剂能部分逆转肺血管结构重建,有效降低肺动脉高压.
배경:폐적공능화결구개변치폐동맥고압시도치폐심병적선결조건,중약폐심합제능구유효강저폐동맥고압,단기궤제상미완전천명.목적:비교불동제량중약폐심합제여초분지평대폐동맥고압대서폐혈관중건적역전효응.설계:이실험동물위연구대상적수궤대조실험연구.단위:일소의과대학부속의원호리계、일소의학원부속의원、일소성급의원중의과.방법:이용야백합감(50 mg/kg)복제대서폐심병모형.웅성Wister대서60지,수궤분위6조:정상대조조、모형조、폐심합제저、중、고제량조급초분지평조,매조10지.분별우제모후14 d관위상응약물,련속용약8 d.처사동물후,이용특수염색결합병리도상분석방법,측정폐소동맥병리급기형태계량학개변.주요관찰지표:①광경관찰급폐소동맥도상분석.②각조폐소동맥관벽중막후도,관벽중막후도여혈관외경비치、관벽면적여혈관총면적비치、관강면적여혈관총면적비치.결과:폐심합제급초분지평균능명현감경모형대서적폐혈관중구(P＜0.01),이폐심합제고제량조효과최호.단치료조각지표잉미완전회복도정상대조조수평.결론:폐심합제능부분역전폐혈관결구중건,유효강저폐동맥고압.
BACKGROUND: Pulmonary hypertension induced by alternation of pulmonary function and structure is the prerequisite of pulmonary-cardiac disorder. Chinese herb, fenxin mixture(FXM) reduces effectively pulmonary hypertension, but the mechanism on which has not been completely explained yet.OBJECTIVE: To comp are the reversal effects between feixin mixture and nifedipine on vascular remodeling in rats with pulmonary hypertension.DESIGN: Randomized controlled experimental study in which experimental animals are taken as the objects.SETTING: Nursing department of affiliated hospital of a medical university;a medical college affiliated hospital and department of traditional Chinese medicine of a provincial hospital.METHODS: Monocrotaline(MCT) (50 mg/kg) was used to duplicate pulmonary-cardiac model in rats. Totally 60 Wister male rats were randomized into 6 groups, namely normal control group, model group, FXM groups of low, middle and high dosages successively and nifedipine group, 10 rats in each group. Since the 14th day of modeling, the corresponding medications were administrated with gastric infusion, continuously for 8 days. After the rats sacrificed, the special staining collaborating with pathological image analysis was used to determine the measurement alternations on path-morphology of pulmonary small artery.wall square(VWS)/total vessel square(TVS), cavity square(CS)/TVS of pulmonary small artery in rats of every group.RESULTS: Both feixin mixture and nifedipine alleviate pulmonary vascular remodeling remarkably( P ＜ 0.01 ), of which, the effect was the best in feixin mixture group of high dosage. But, the every index in experimental groups had not recovered to the level in normal control group completely.CONCLUSION: Feixin mixture reverses partially the remodeling of pulmonary vascular structure and reduces effectively pulmonary hypertension.