CAJ | 학술논문

在已知GCYC基因部分序列基础上, 通过改进的mTAIL-PCR方法克隆非洲紫罗兰Saintpaulia ionantha两侧对称栽培种中CYC类基因的5′未知序列, 并进而从两侧与辐射对称栽培种中分离得到苦苣苔科Gesneriaceae中第一组完整基因: SiCYC1A与SiCYC1B.对以上基因的核酸和氨基酸序列比较发现, SiCYC1A与SiCYC1B序列同源性很高, 均含有完整的功能调控区域(即TCP domain和R domain)并与模式植物金鱼草Antirrhinum majus中CYC基因同源.因此, 这两个基因应具有正常功能, 是功能上互补的冗余基因.令人意外的是在辐射对称花栽培品种中的这两个基因和两侧对称花栽培品种中对应基因的序列完全相同.经过对金鱼草以及相关类群辐射对称花突变体中CYC类基因序列的比较分析, 推论在非洲紫罗兰中, SiCYC1A与SiCYC1B基因可能受上游未知的共同调控因子调控, 该调控因子的改变是导致栽培品种中花对称性发生变化的主要原因.另外, 对改进后的TAIL-PCR(mTAIL-PCR)的方法和过程进行了详细叙述, 并对其技术特征和优势开展了简单的论述.
재이지GCYC기인부분서렬기출상, 통과개진적mTAIL-PCR방법극륭비주자라란Saintpaulia ionantha량측대칭재배충중CYC류기인적5′미지서렬, 병진이종량측여복사대칭재배충중분리득도고거태과Gesneriaceae중제일조완정기인: SiCYC1A여SiCYC1B.대이상기인적핵산화안기산서렬비교발현, SiCYC1A여SiCYC1B서렬동원성흔고, 균함유완정적공능조공구역(즉TCP domain화R domain)병여모식식물금어초Antirrhinum majus중CYC기인동원.인차, 저량개기인응구유정상공능, 시공능상호보적용여기인.령인의외적시재복사대칭화재배품충중적저량개기인화량측대칭화재배품충중대응기인적서렬완전상동.경과대금어초이급상관류군복사대칭화돌변체중CYC류기인서렬적비교분석, 추론재비주자라란중, SiCYC1A여SiCYC1B기인가능수상유미지적공동조공인자조공, 해조공인자적개변시도치재배품충중화대칭성발생변화적주요원인.령외, 대개진후적TAIL-PCR(mTAIL-PCR)적방법화과정진행료상세서술, 병대기기술특정화우세개전료간단적논술.
Using the mTAIL-PCR method, we have isolated the two CYC-like genes, SiCYC1A and SiCYC1B, from zygomorphic and actinomorphic cultivars of Saintpaulia ionantha respectively in Gesneriaceae. The two genes, SiCYC1A and SiCYC1B, from the zygomorphic cultivar both contain the whole regulation domain, i.e. TCP and R domains. Therefore, they should be functional in the floral symmetry establishment, homologous with CYC in Antirrhinum majus. Unexpectedly, the two genes from the actinomorphic cultivar are identical to those from the zygomorphic in DNA sequence, respectively. Based on comparative analysis of the molecular alteration at CYC-like genes, which are responsible for the morphological transformation from zygomorphy to actinomorphy, we suggest that the two closely related genes SiCYC1A and SiCYC1B might be regulated by a common upstream regulator, whose change would result in silence of both SiCYC1A and SiCYC1B in controlling the development of the adaxial and lateral organs in a flower. In addition, an mTAIL-PCR method was shown to have the technological advantages of the unknown sequence for isolation.