北京大学学报（医学版）
北京大學學報（醫學版）
북경대학학보（의학판）
JOURNAL OF BEIJING MEDICAL UNIVERSITY(HEALTH SCIENCES)
2009年
4期
402-408
,共7页
霍丽蓉%申晨%Wei-na JU%邹俊华%闫武%W.Ted BROWN%Nanbert ZHONG
霍麗蓉%申晨%Wei-na JU%鄒俊華%閆武%W.Ted BROWN%Nanbert ZHONG
곽려용%신신%Wei-na JU%추준화%염무%W.Ted BROWN%Nanbert ZHONG
Poly(C)结合蛋白质1%酵母菌%杂交,遗传%蛋白质结合
Poly(C)結閤蛋白質1%酵母菌%雜交,遺傳%蛋白質結閤
Poly(C)결합단백질1%효모균%잡교,유전%단백질결합
Poly(C)-binding protein 1%Yeasts%Hybridization,genetic%Protein binding
Objective: PCBP1 is a family member of heterogeneous nuclear ribonucleoproteins (hnRNPs) that belong to RNA-binding proteins and bear three KH domains. The protein plays a pivotal role in post-transcriptional regulation for RNA metabolism and RNA function in gene expression. We hy-pothesized and were going to identify that the regulatory function of PCBP1 is performed through different complexes of proteins that include PCBP1. Methods: To test our hypothesis, approaches of protein wal-king with a yeast two-hybrid system (Y2H), pulling down in yeasts, co-immunoprecipitation and immu-nofluorescent microscopy assay were employed in this study. The PCBP1 was used as the initial "walker" to search for its interaction partner(s). Results: Candidate proteins including MYL6, PECAM1, CSH1,RAB7, p57KIP2, ACTG1, RBMS1 and PSG4-1ike were identified with selection mediums and preceding methods. Conclusion: With these candidate protein molecules, some protein complexes associating with PCBP1 are proposed, which may help in a better understanding of physiological functions of PCBP1 and proved evidence that PCBP1 is involved in variant biological pathways.
