CAJ | 학술논문

以牡丹品种'凤丹'的带花丝的花药为外植体,诱导其产生愈伤组织,利用不同发育时期的花药诱导愈伤组织,确定了最佳的花药发育时期为单核中期,利用单核中期的花药,在确定合适激素配比的基础上,从不同的蔗糖质量浓度、4℃条件下不同处理时间等方面对花药愈伤组织诱导的影响进行了系统研究.结果表明:花药诱导愈伤组织合适的激素组合为MS+2,4-D2.0mg·L~(-1)+6-BA1.5mg·L~(-1)+NAA1.0mg·L~(-1);花药在蔗糖质量浓度为6%、4℃条件下处理8d时,愈伤组织的诱导率最高可达50.8%,优化了牡丹花药愈伤组织培养组合.
이모단품충'봉단'적대화사적화약위외식체,유도기산생유상조직,이용불동발육시기적화약유도유상조직,학정료최가적화약발육시기위단핵중기,이용단핵중기적화약,재학정합괄격소배비적기출상,종불동적자당질량농도、4℃조건하불동처리시간등방면대화약유상조직유도적영향진행료계통연구.결과표명:화약유도유상조직합괄적격소조합위MS+2,4-D2.0mg·L~(-1)+6-BA1.5mg·L~(-1)+NAA1.0mg·L~(-1);화약재자당질량농도위6%、4℃조건하처리8d시,유상조직적유도솔최고가체50.8%,우화료모단화약유상조직배양조합.
An experiment was conducted to study callus induction from anther with stalk using Paeonia suffruticosa'fengdan'as explants. Effects of concentration of growth regulators, sucrose content, and cold pre treatment time on callus induction were studied at different periods of anther development. Results show that the best period for callus induction of anther is the middle developmental stage, the optimal growth regulator is MS supplemented with 2.0mg·L~(-1) 2,4-D, 1.5mg·L~(-1) 6-BA, and 1.0mg·L~(-1) NAA, and the best sucrose content is 6%. After 8 days of cold pre treatment at 4 degrees C, the callus grew well, and the induction efficiency reached 50.8%.