昆虫学报
昆蟲學報
곤충학보
ACTA ENTOMOLOGICA SINICA
2009年
12期
1379-1384
,共6页
路常宽%王晓勤%张巨山%赵铁栋
路常寬%王曉勤%張巨山%趙鐵棟
로상관%왕효근%장거산%조철동
苹毛丽金龟%植物挥发物%EAG反应%行为反应%Y型嗅觉仪
蘋毛麗金龜%植物揮髮物%EAG反應%行為反應%Y型嗅覺儀
평모려금구%식물휘발물%EAG반응%행위반응%Y형후각의
Proagopertha lucidula%plant volatiles%EAG response%behavioral response%Y-tube olfactometer
为筛选适宜的苹毛丽金龟 Proagopertha lucidula (Faldermann)引诱剂化学物质,对已报道的金龟子科两性引诱剂单一组分进行了触角电位(EAG)和Y型嗅觉仪中的嗅觉反应测定.结果表明:在10 μg刺激剂量下测试的11种植物挥发物中,只有顺-3-己烯-1-醇、香叶醇、茴香脑和丁香酚有明确的EAG反应值,其他7种化合物具有弱反应值.剂量反应曲线表明,具有明确的EAG活性的4种化合物激发的反应强度与化合物的剂量呈正相关.在Y型嗅觉仪中,顺-3-己烯-1-醇、香叶醇、茴香脑和丁香酚4种化合物在1,10,100 μg剂量下的行为反应百分率都显著高于对照溶剂正己烷,且雌雄之间无显著差异.该研究结果可为开发苹毛丽金龟田间引诱剂奠定基础.
為篩選適宜的蘋毛麗金龜 Proagopertha lucidula (Faldermann)引誘劑化學物質,對已報道的金龜子科兩性引誘劑單一組分進行瞭觸角電位(EAG)和Y型嗅覺儀中的嗅覺反應測定.結果錶明:在10 μg刺激劑量下測試的11種植物揮髮物中,隻有順-3-己烯-1-醇、香葉醇、茴香腦和丁香酚有明確的EAG反應值,其他7種化閤物具有弱反應值.劑量反應麯線錶明,具有明確的EAG活性的4種化閤物激髮的反應彊度與化閤物的劑量呈正相關.在Y型嗅覺儀中,順-3-己烯-1-醇、香葉醇、茴香腦和丁香酚4種化閤物在1,10,100 μg劑量下的行為反應百分率都顯著高于對照溶劑正己烷,且雌雄之間無顯著差異.該研究結果可為開髮蘋毛麗金龜田間引誘劑奠定基礎.
위사선괄의적평모려금구 Proagopertha lucidula (Faldermann)인유제화학물질,대이보도적금구자과량성인유제단일조분진행료촉각전위(EAG)화Y형후각의중적후각반응측정.결과표명:재10 μg자격제량하측시적11충식물휘발물중,지유순-3-기희-1-순、향협순、회향뇌화정향분유명학적EAG반응치,기타7충화합물구유약반응치.제량반응곡선표명,구유명학적EAG활성적4충화합물격발적반응강도여화합물적제량정정상관.재Y형후각의중,순-3-기희-1-순、향협순、회향뇌화정향분4충화합물재1,10,100 μg제량하적행위반응백분솔도현저고우대조용제정기완,차자웅지간무현저차이.해연구결과가위개발평모려금구전간인유제전정기출.
In order to screen attractive chemicals for trapping males and females Proagopertha lucidula (Faldermann), 11 plant volatiles that can trap male and female adults of other scarabs were used to test the electroantennogram (EAG) value and the taxis behavior of P. lucidula adults. The results showed that among the 11 test chemicals at the dosage of 10 μg, only (Z)-3-hexen-l-ol, geraniol, anethol and eugenol induced clear EAG response, while the other 7 chemicals elicited weak EAG response. The four compounds that induced clear EAG responses at the dose of 10 μg/μL elicited the dose-dependent EAG response. The taxis behavior of the beetle to the volatiles that induced clear EAG responses was also assayed at the doses of 1, 10 and 100 μg with Y-tube olfactometer. Corrected response percentages of both males and females to (Z)-3-hexen-l-ol, geraniol anethol and eugenol were significantly different from that of the hexane control. The study will provide the foundation for developing attractive chemicals for P. lucidula in fields.