光谱实验室
光譜實驗室
광보실험실
CHINESE JOURNAL OF SPECTROSCOPY LABORATORY
2010年
2期
645-647
,共3页
溴化十六烷基三甲基铵%二甲苯兰FF%核酸%共振光散射
溴化十六烷基三甲基銨%二甲苯蘭FF%覈痠%共振光散射
추화십륙완기삼갑기안%이갑분란FF%핵산%공진광산사
Cetyhrimethyl Ammonium Bromide%Xylene Cyanal FF%Nucleic Acid%Resonance Light Scattering
在pH 9.90的Tris-HCl缓冲溶液中.阴离子染料二甲苯兰FF(XCFF)对阳离子表面活性剂溴化十六烷基三甲基铵(CTMAB)与核酸(fsDNA,ctDNA、yRNA)的共振光散射光谱有协同增强作用.考察了影响因素,在优化条件下研究了共振光散射强度与核酸浓度之间的关系,对于fsDNA、ctDNA、yRNA的线性范围分别为0.05-3.Omg/L、0.05-6.0mg/L、0.5-2.0mg/L,检出限分别为24.1、18.4、57.0μg/L.据此建立了一种测定核酸的新方法.
在pH 9.90的Tris-HCl緩遲溶液中.陰離子染料二甲苯蘭FF(XCFF)對暘離子錶麵活性劑溴化十六烷基三甲基銨(CTMAB)與覈痠(fsDNA,ctDNA、yRNA)的共振光散射光譜有協同增彊作用.攷察瞭影響因素,在優化條件下研究瞭共振光散射彊度與覈痠濃度之間的關繫,對于fsDNA、ctDNA、yRNA的線性範圍分彆為0.05-3.Omg/L、0.05-6.0mg/L、0.5-2.0mg/L,檢齣限分彆為24.1、18.4、57.0μg/L.據此建立瞭一種測定覈痠的新方法.
재pH 9.90적Tris-HCl완충용액중.음리자염료이갑분란FF(XCFF)대양리자표면활성제추화십륙완기삼갑기안(CTMAB)여핵산(fsDNA,ctDNA、yRNA)적공진광산사광보유협동증강작용.고찰료영향인소,재우화조건하연구료공진광산사강도여핵산농도지간적관계,대우fsDNA、ctDNA、yRNA적선성범위분별위0.05-3.Omg/L、0.05-6.0mg/L、0.5-2.0mg/L,검출한분별위24.1、18.4、57.0μg/L.거차건립료일충측정핵산적신방법.
Resonance light-scattering (RLS) spectra of the xylene cyanal FF (XCFF)-cetyltrimethyl ammonium bromide(CTMAB)-nucleic acid system has been studied.In Tris-HCl buffer solution (pH = 9.90),XCFF and nucleic acid react with CTMAB to form large particles of ion-association complex, which result in significant enhancement of RLS intensity.The spectral characteristics of RLS, the effective factors and optimum conditions of the reaction have been investigated.Under the optimum conditions, the enhanced RLS intensity is proportional to the concentration of nucleic acid in the range of 0.05 to 3.0mg/L,0.05 to 6.0mg/L,0.5 to 2.0mg/L for the fsDNA, ctDNA, and yRNA respectively.The detection limits are 24.1μg/L, 18.4μg/L and 57.0μg/L respectively.
