CAJ | 학술논문

对186份大豆种质资源进行了成株和种粒斑驳抗性鉴定,并利用与成株抗性及种粒斑驳抗性分别相关的SSR标记验证抗藕毒分子辅助选择的可行性.结果表明:接种SMV1,选出成株和种粒双抗种质38份,成株抗病种质149份,种粒抗病种质45份,成株和种粒双感种质26份.利用与成株抗性相关的SSR标记Sat_229、Sat_317、Satt335、Satt160、Satt516、Sat_309进行检测,抗病毒资源筛选的准确率分别达到68.9%、74.3%、71.1%、69.8%、77.4%和68.2%.利用与种粒斑驳抗性相关的SSR标记Sat_297、Sat_229、Sat_317、Satt335、Sct_188、Satt160、Satt516、Sat_133进行检测,Sat_317标记准确率达79.1%,标记Sat_229、Satt335、Satt516和Sat_133抗病毒资源筛选的准确率均达70%以上,可以用作抗病毒分子辅助育种的选择标记.
대186빈대두충질자원진행료성주화충립반박항성감정,병이용여성주항성급충립반박항성분별상관적SSR표기험증항우독분자보조선택적가행성.결과표명:접충SMV1,선출성주화충립쌍항충질38빈,성주항병충질149빈,충립항병충질45빈,성주화충립쌍감충질26빈.이용여성주항성상관적SSR표기Sat_229、Sat_317、Satt335、Satt160、Satt516、Sat_309진행검측,항병독자원사선적준학솔분별체도68.9%、74.3%、71.1%、69.8%、77.4%화68.2%.이용여충립반박항성상관적SSR표기Sat_297、Sat_229、Sat_317、Satt335、Sct_188、Satt160、Satt516、Sat_133진행검측,Sat_317표기준학솔체79.1%,표기Sat_229、Satt335、Satt516화Sat_133항병독자원사선적준학솔균체70%이상,가이용작항병독분자보조육충적선택표기.
One hundred and eighty six soybean germplasms were screened for resistance to adult-plant and seed coat mottling by the inoculation of strain SMV1, and the coincident rate between adult-plant resistance, seed coat mottling resistance to SMV and linkage SSR markers were evaluated. The results showed that among these 186 germplasms, 38 were resistant to both adult-plant and seed coat mottling; 149 were only resistant to adult-plant; 45 were only resistant to seed coat mottling; and 26 were both susceptible to adult-plant and seed coat mottling. 6 SSR markers, Sat_229, Sat_317, Satt335, Satt160, Satt516 and Sat_309, related to SMV adult-plant resistance, were used to identify the 186 soybean germplasms. The coincident rates between the SSR alleles and the resistant presence were 68. 9%, 74. 3%, 71. 1%, 69. 8%, 77. 4%and 68. 2%, respectively; 8 SSR markers, Sat_297, Sat_229, Sat_317, Satt335, Sct_188, Satt160, Satt516 and Sat_133, related to SMV seed coat mottling resistance, were used to identify the 186 soybean germplasms, the coincident rates of Sat_317 alleles with the resistant presence were 79. 1%, and that of Sat_229, Satt335, Satt516 and Sat_133 were all over 70%. These markers related to SMV1 resistance were available for the marker assisted selection for SMV resistance breeding.