CAJ | 학술논문

目的探讨化疗药物对胃癌细胞的杀伤效应.方法收集2006年4月至2007年2月济南军区总医院手术切除的84例胃癌标本,新鲜胃癌组织制备单细胞悬液,分别加入羟基喜树碱、顺铂、阿霉素、5-氟尿嘧啶和丝裂霉素培养48 h.MTT法观察药物作用后癌细胞活性变化.免疫组织化学检测生存素及PTEN的表达.采用χ~2、秩和检验和Fisher确切概率法分析数据.结果不同化疗药物对胃癌细胞杀伤效应不同,分化差的胃癌较分化好的胃癌对化疗药物敏感.在印戒细胞癌、黏液腺癌及低分化腺癌中生存素阳性率高于乳头状腺癌及管状腺癌,差异有统计学意义(χ~2=10.625,P<0.05);而FFEN表达与生存素相反,差异有统计学意义(χ~2=6.060,P<0.05).生存素与5-氟尿嘧啶及阿霉素的体外耐药有关(χ~2=6.609,6.350,P<0.05).结论 MTT体外药敏实验有助于筛选个体有效化疗药物.生存素参与了肿瘤细胞化疗耐受,生存素与PTEN的表达有关.
목적 탐토화료약물대위암세포적살상효응.방법 수집2006년4월지2007년2월제남군구총의원수술절제적84례위암표본,신선위암조직제비단세포현액,분별가입간기희수감、순박、아매소、5-불뇨밀정화사렬매소배양48 h.MTT법관찰약물작용후암세포활성변화.면역조직화학검측생존소급PTEN적표체.채용χ~2、질화검험화Fisher학절개솔법분석수거.결과 불동화료약물대위암세포살상효응불동,분화차적위암교분화호적위암대화료약물민감.재인계세포암、점액선암급저분화선암중생존소양성솔고우유두상선암급관상선암,차이유통계학의의(χ~2=10.625,P<0.05);이FFEN표체여생존소상반,차이유통계학의의(χ~2=6.060,P<0.05).생존소여5-불뇨밀정급아매소적체외내약유관(χ~2=6.609,6.350,P<0.05).결론 MTT체외약민실험유조우사선개체유효화료약물.생존소삼여료종류세포화료내수,생존소여PTEN적표체유관.
Objective To evaluate the anti-tumor effects of chemotherapeutic drugs on human gastric cancer cells. Methods From April 2006 to February 2007, 84 patients with gastric cancer underwent surgical resection at General Hospital of Jinan Military Command. The single-cell suspension of these gastric tumors was prepared. The gastric cancer cells were cultured with hydroxycamptothecin, cisplatin, adriamycin, 5-fluorouracil and mitomycin for 48 hours, and changes in activity of the gastric cancer cells were studied via MTT assay. The expression of survivin and PTEN was detected by immunohistochemistry. Data were analyzed by chi-square test, rank sum test and Fisher exact test. Results The anti-tumor effects of different chemotherapeutic drugs were different, and the poorly-differentiated gastric cancer cells were more sensitive to the cytotoxic effects of chemotherapeutic drugs than the well-differentiated gastric cancer cells. The expression levels of survivin in the signet ring cell carcinoma, mucinous adenocarcinoma and other poorly-differentiated adenocarcinoma were significantly higher than those in papillary carcinoma and tubular carcinoma (χ~2 = 10.625, P <0.05), while the expression of PTEN was inverse to that of survivin (χ~2 = 6.060, P < 0.05). The expression of survivin was related to the resistance of the gastric cancer cells to 5-fluorouracil and adriamycin (χ~2 = 6.609, 6.350, P < 0.05). Conclusions In vitro chemosensitivity assay is helpful in selecting the chemotherapeutic regimen for specific types of gastric cancer. Survivin may contribute to the chemotherapy-resistance of certain types of gastric cancer cells, and its expression is related to that of PTEN.