中国实用眼科杂志
中國實用眼科雜誌
중국실용안과잡지
CHINESE JOURNAL OF PRACTICAL OPHTHALMOLOGY
2011年
3期
278-280
,共3页
视网膜%米诺环素%慢性高眼压%兔
視網膜%米諾環素%慢性高眼壓%兔
시망막%미낙배소%만성고안압%토
Retinal%Minocycline%Chronic ocular hypertension%Rabbit
目的 探索米诺环素对兔慢性高眼压下视网膜组织损伤的保护作用.方法 成年家兔48只,随机分为正常组、高眼压组和高眼压治疗组,后2组每只兔右眼前房内注射3g/L复方卡波姆溶液0.1mL,造慢性高眼压模型.造模成功后,治疗组口服米诺环素胶囊50mg/(kg·d).每周相同时间点测眼压,第28d对所有动物视网膜相应部位行光学相干断层扫描(OCT)后处死各组动物,检测视网膜超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)含量,并进行组间比较分析.结果 后两组右眼比较,各时间点眼压差异无统计学意义(P＞0.05).治疗组OCT检查法测得的视网膜厚度及SOD值明显高于高眼压组;NO和MDA明显低于高眼压组(P＜0.05).结论 米诺环素可以维持慢性高眼压作用下的视网膜厚度,调控SOD、NO、MDA对慢性高眼压下兔视网膜组织起到保护作用.
目的 探索米諾環素對兔慢性高眼壓下視網膜組織損傷的保護作用.方法 成年傢兔48隻,隨機分為正常組、高眼壓組和高眼壓治療組,後2組每隻兔右眼前房內註射3g/L複方卡波姆溶液0.1mL,造慢性高眼壓模型.造模成功後,治療組口服米諾環素膠囊50mg/(kg·d).每週相同時間點測眼壓,第28d對所有動物視網膜相應部位行光學相榦斷層掃描(OCT)後處死各組動物,檢測視網膜超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)含量,併進行組間比較分析.結果 後兩組右眼比較,各時間點眼壓差異無統計學意義(P＞0.05).治療組OCT檢查法測得的視網膜厚度及SOD值明顯高于高眼壓組;NO和MDA明顯低于高眼壓組(P＜0.05).結論 米諾環素可以維持慢性高眼壓作用下的視網膜厚度,調控SOD、NO、MDA對慢性高眼壓下兔視網膜組織起到保護作用.
목적 탐색미낙배소대토만성고안압하시망막조직손상적보호작용.방법 성년가토48지,수궤분위정상조、고안압조화고안압치료조,후2조매지토우안전방내주사3g/L복방잡파모용액0.1mL,조만성고안압모형.조모성공후,치료조구복미낙배소효낭50mg/(kg·d).매주상동시간점측안압,제28d대소유동물시망막상응부위행광학상간단층소묘(OCT)후처사각조동물,검측시망막초양화물기화매(SOD)、병이철(MDA)、일양화담(NO)함량,병진행조간비교분석.결과 후량조우안비교,각시간점안압차이무통계학의의(P＞0.05).치료조OCT검사법측득적시망막후도급SOD치명현고우고안압조;NO화MDA명현저우고안압조(P＜0.05).결론 미낙배소가이유지만성고안압작용하적시망막후도,조공SOD、NO、MDA대만성고안압하토시망막조직기도보호작용.
Objective To observe the protective effect of Minocycline in chronic ocular hypertenstion models.Methods Forty-eight rabbits were randomly divided into three groups:control,chronic ocular hypertension,and Minocycline group.The right eye of chronic ocular hypertension and Minocycline group were used to build chronic ocular hypertension model by posterior chamber injection in right eye of 0.l ml of 3g/L Carbomer solution.After modeling,Minocycline group rabbits took Minocycline every day 50mg/kg.The intraocular pressure (IOP) was monitored every week at the same time point.The retinal thickness was measured by OCT at 28 days after feed the medicine.Then the animals were sacrificed.The contents of malondiadehyde (MDA),superoxide dismutase (SOD) and NO in retina were assayed and compared among groups.Result The IOP in chronic ocular hypertension,and Minocycline group increased and they had no obvious difference (P ＞0.05).The retinal thickness and the level of SOD in retinal tissue in chronic ocular hypertension group was markedly lower than that in Minocycline group (P ＜0.05),the content of MDA and NO were obviously higher than that in Minocycline group (P ＜0.05).Conclusions Minocycline can protect retinal neurons by maintaining regulating retinal thickness and production of SOD,MDA,NO in chronic ocular hypertension rabbits model.
